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miR-181a Regulates Inflammation Responses in Monocytes and Macrophages
miR-181a has been presumed to target the 3′-untranslated regions (3′-UTR) of IL1a based on software predictions. miR-181a and IL1a have opposite expression levels in monocytes and macrophages in the inflammatory state. This led us to suspect that mir-181a has an important function in regulating infl...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2013
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3596280/ https://www.ncbi.nlm.nih.gov/pubmed/23516523 http://dx.doi.org/10.1371/journal.pone.0058639 |
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author | Xie, Weidong Li, Mengnan Xu, Naihan Lv, Qing Huang, Nunu He, Jie Zhang, Yaou |
author_facet | Xie, Weidong Li, Mengnan Xu, Naihan Lv, Qing Huang, Nunu He, Jie Zhang, Yaou |
author_sort | Xie, Weidong |
collection | PubMed |
description | miR-181a has been presumed to target the 3′-untranslated regions (3′-UTR) of IL1a based on software predictions. miR-181a and IL1a have opposite expression levels in monocytes and macrophages in the inflammatory state. This led us to suspect that mir-181a has an important function in regulating inflammatory response by targeting IL1a. Fluorescence reporter assays showed that miR-181a effectively binds to the 3′-UTR of IL1a. The anti-inflammatory functions of miR-181a were investigated in lipopolysaccharides (LPS)-induced Raw264.7 and phorbol 12-myristate 13-acetate (PMA)/LPS-induced THP-1 cells. We found that miR-181a mimics significantly lowered IL1a expression levels in these cells and, interestingly, miR-181a inhibitors reversed this decrease. In addition, miR-181a mimics significantly inhibited increase in the levels of inflammatory factors (IL1b, IL6, and TNFa) in these cells. Furthermore, miR-181a mimics and inhibitors decreased and increased, respectively, production of reactive oxygen species in PMA/LPS-induced THP-1 cells. These results indicate that miR-181a regulates inflammatory responses by directly targeting the 3′-UTR of IL1a and down-regulating IL1a levels. Interestingly, we found that miR-181a inhibited production of inflammatory factors even in IL1a-induced THP-1 cells, suggesting that the anti-inflammatory effects of miR-181a possibly involves other targets in addition to IL1a. Thus, we provide the first evidence for anti-inflammatory effects of miR-181a mediated at least in part by down-regulating IL1a. |
format | Online Article Text |
id | pubmed-3596280 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2013 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-35962802013-03-20 miR-181a Regulates Inflammation Responses in Monocytes and Macrophages Xie, Weidong Li, Mengnan Xu, Naihan Lv, Qing Huang, Nunu He, Jie Zhang, Yaou PLoS One Research Article miR-181a has been presumed to target the 3′-untranslated regions (3′-UTR) of IL1a based on software predictions. miR-181a and IL1a have opposite expression levels in monocytes and macrophages in the inflammatory state. This led us to suspect that mir-181a has an important function in regulating inflammatory response by targeting IL1a. Fluorescence reporter assays showed that miR-181a effectively binds to the 3′-UTR of IL1a. The anti-inflammatory functions of miR-181a were investigated in lipopolysaccharides (LPS)-induced Raw264.7 and phorbol 12-myristate 13-acetate (PMA)/LPS-induced THP-1 cells. We found that miR-181a mimics significantly lowered IL1a expression levels in these cells and, interestingly, miR-181a inhibitors reversed this decrease. In addition, miR-181a mimics significantly inhibited increase in the levels of inflammatory factors (IL1b, IL6, and TNFa) in these cells. Furthermore, miR-181a mimics and inhibitors decreased and increased, respectively, production of reactive oxygen species in PMA/LPS-induced THP-1 cells. These results indicate that miR-181a regulates inflammatory responses by directly targeting the 3′-UTR of IL1a and down-regulating IL1a levels. Interestingly, we found that miR-181a inhibited production of inflammatory factors even in IL1a-induced THP-1 cells, suggesting that the anti-inflammatory effects of miR-181a possibly involves other targets in addition to IL1a. Thus, we provide the first evidence for anti-inflammatory effects of miR-181a mediated at least in part by down-regulating IL1a. Public Library of Science 2013-03-13 /pmc/articles/PMC3596280/ /pubmed/23516523 http://dx.doi.org/10.1371/journal.pone.0058639 Text en © 2013 Xie et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited. |
spellingShingle | Research Article Xie, Weidong Li, Mengnan Xu, Naihan Lv, Qing Huang, Nunu He, Jie Zhang, Yaou miR-181a Regulates Inflammation Responses in Monocytes and Macrophages |
title | miR-181a Regulates Inflammation Responses in Monocytes and Macrophages |
title_full | miR-181a Regulates Inflammation Responses in Monocytes and Macrophages |
title_fullStr | miR-181a Regulates Inflammation Responses in Monocytes and Macrophages |
title_full_unstemmed | miR-181a Regulates Inflammation Responses in Monocytes and Macrophages |
title_short | miR-181a Regulates Inflammation Responses in Monocytes and Macrophages |
title_sort | mir-181a regulates inflammation responses in monocytes and macrophages |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3596280/ https://www.ncbi.nlm.nih.gov/pubmed/23516523 http://dx.doi.org/10.1371/journal.pone.0058639 |
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