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miR-181a Regulates Inflammation Responses in Monocytes and Macrophages

miR-181a has been presumed to target the 3′-untranslated regions (3′-UTR) of IL1a based on software predictions. miR-181a and IL1a have opposite expression levels in monocytes and macrophages in the inflammatory state. This led us to suspect that mir-181a has an important function in regulating infl...

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Autores principales: Xie, Weidong, Li, Mengnan, Xu, Naihan, Lv, Qing, Huang, Nunu, He, Jie, Zhang, Yaou
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3596280/
https://www.ncbi.nlm.nih.gov/pubmed/23516523
http://dx.doi.org/10.1371/journal.pone.0058639
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author Xie, Weidong
Li, Mengnan
Xu, Naihan
Lv, Qing
Huang, Nunu
He, Jie
Zhang, Yaou
author_facet Xie, Weidong
Li, Mengnan
Xu, Naihan
Lv, Qing
Huang, Nunu
He, Jie
Zhang, Yaou
author_sort Xie, Weidong
collection PubMed
description miR-181a has been presumed to target the 3′-untranslated regions (3′-UTR) of IL1a based on software predictions. miR-181a and IL1a have opposite expression levels in monocytes and macrophages in the inflammatory state. This led us to suspect that mir-181a has an important function in regulating inflammatory response by targeting IL1a. Fluorescence reporter assays showed that miR-181a effectively binds to the 3′-UTR of IL1a. The anti-inflammatory functions of miR-181a were investigated in lipopolysaccharides (LPS)-induced Raw264.7 and phorbol 12-myristate 13-acetate (PMA)/LPS-induced THP-1 cells. We found that miR-181a mimics significantly lowered IL1a expression levels in these cells and, interestingly, miR-181a inhibitors reversed this decrease. In addition, miR-181a mimics significantly inhibited increase in the levels of inflammatory factors (IL1b, IL6, and TNFa) in these cells. Furthermore, miR-181a mimics and inhibitors decreased and increased, respectively, production of reactive oxygen species in PMA/LPS-induced THP-1 cells. These results indicate that miR-181a regulates inflammatory responses by directly targeting the 3′-UTR of IL1a and down-regulating IL1a levels. Interestingly, we found that miR-181a inhibited production of inflammatory factors even in IL1a-induced THP-1 cells, suggesting that the anti-inflammatory effects of miR-181a possibly involves other targets in addition to IL1a. Thus, we provide the first evidence for anti-inflammatory effects of miR-181a mediated at least in part by down-regulating IL1a.
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spelling pubmed-35962802013-03-20 miR-181a Regulates Inflammation Responses in Monocytes and Macrophages Xie, Weidong Li, Mengnan Xu, Naihan Lv, Qing Huang, Nunu He, Jie Zhang, Yaou PLoS One Research Article miR-181a has been presumed to target the 3′-untranslated regions (3′-UTR) of IL1a based on software predictions. miR-181a and IL1a have opposite expression levels in monocytes and macrophages in the inflammatory state. This led us to suspect that mir-181a has an important function in regulating inflammatory response by targeting IL1a. Fluorescence reporter assays showed that miR-181a effectively binds to the 3′-UTR of IL1a. The anti-inflammatory functions of miR-181a were investigated in lipopolysaccharides (LPS)-induced Raw264.7 and phorbol 12-myristate 13-acetate (PMA)/LPS-induced THP-1 cells. We found that miR-181a mimics significantly lowered IL1a expression levels in these cells and, interestingly, miR-181a inhibitors reversed this decrease. In addition, miR-181a mimics significantly inhibited increase in the levels of inflammatory factors (IL1b, IL6, and TNFa) in these cells. Furthermore, miR-181a mimics and inhibitors decreased and increased, respectively, production of reactive oxygen species in PMA/LPS-induced THP-1 cells. These results indicate that miR-181a regulates inflammatory responses by directly targeting the 3′-UTR of IL1a and down-regulating IL1a levels. Interestingly, we found that miR-181a inhibited production of inflammatory factors even in IL1a-induced THP-1 cells, suggesting that the anti-inflammatory effects of miR-181a possibly involves other targets in addition to IL1a. Thus, we provide the first evidence for anti-inflammatory effects of miR-181a mediated at least in part by down-regulating IL1a. Public Library of Science 2013-03-13 /pmc/articles/PMC3596280/ /pubmed/23516523 http://dx.doi.org/10.1371/journal.pone.0058639 Text en © 2013 Xie et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Xie, Weidong
Li, Mengnan
Xu, Naihan
Lv, Qing
Huang, Nunu
He, Jie
Zhang, Yaou
miR-181a Regulates Inflammation Responses in Monocytes and Macrophages
title miR-181a Regulates Inflammation Responses in Monocytes and Macrophages
title_full miR-181a Regulates Inflammation Responses in Monocytes and Macrophages
title_fullStr miR-181a Regulates Inflammation Responses in Monocytes and Macrophages
title_full_unstemmed miR-181a Regulates Inflammation Responses in Monocytes and Macrophages
title_short miR-181a Regulates Inflammation Responses in Monocytes and Macrophages
title_sort mir-181a regulates inflammation responses in monocytes and macrophages
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3596280/
https://www.ncbi.nlm.nih.gov/pubmed/23516523
http://dx.doi.org/10.1371/journal.pone.0058639
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