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An Investigation of Fecal Volatile Organic Metabolites in Irritable Bowel Syndrome
Diagnosing irritable bowel syndrome (IBS) can be a challenge; many clinicians resort to invasive investigations in order to rule out other diseases and reassure their patients. Volatile organic metabolites (VOMs) are emitted from feces; understanding changes in the patterns of these VOMs could aid o...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2013
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3596408/ https://www.ncbi.nlm.nih.gov/pubmed/23516449 http://dx.doi.org/10.1371/journal.pone.0058204 |
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author | Ahmed, Iftikhar Greenwood, Rosemary Costello, Ben de Lacy Ratcliffe, Norman M. Probert, Chris S. |
author_facet | Ahmed, Iftikhar Greenwood, Rosemary Costello, Ben de Lacy Ratcliffe, Norman M. Probert, Chris S. |
author_sort | Ahmed, Iftikhar |
collection | PubMed |
description | Diagnosing irritable bowel syndrome (IBS) can be a challenge; many clinicians resort to invasive investigations in order to rule out other diseases and reassure their patients. Volatile organic metabolites (VOMs) are emitted from feces; understanding changes in the patterns of these VOMs could aid our understanding of the etiology of the disease and the development of biomarkers, which can assist in the diagnosis of IBS. We report the first comprehensive study of the fecal VOMs patterns in patients with diarrhea-predominant IBS (IBS-D), active Crohn's disease (CD), ulcerative colitis (UC) and healthy controls. 30 patients with IBS-D, 62 with CD, 48 with UC and 109 healthy controls were studied. Diagnosis of IBS-D was made using the Manning criteria and all patients with CD and UC met endoscopic, histologic and/or radiologic criteria. Fecal VOMs were extracted by solid phase microextraction (SPME) and analyzed by gas chromatography-mass spectrometry (GC-MS). 240 VOMs were identified. Univariate analysis showed that esters of short chain fatty acids, cyclohexanecarboxylic acid and its ester derivatives were associated with IBS-D (p<0.05), while aldehydes were more abundant in IBD (p<0.05). A predictive model, developed by multivariate analysis, separated IBS-D from active CD, UC and healthy controls with a sensitivity of 94%, 96% and 90%; and a specificity of 82%, 80% and 80% respectively (p<0.05). The understanding of the derivation of these VOMs may cast light on the etiology of IBS-D and IBD. These data show that fecal VOMs analyses could contribute to the diagnosis of IBS-D, for which there is no laboratory test, as well as IBD. |
format | Online Article Text |
id | pubmed-3596408 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2013 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-35964082013-03-20 An Investigation of Fecal Volatile Organic Metabolites in Irritable Bowel Syndrome Ahmed, Iftikhar Greenwood, Rosemary Costello, Ben de Lacy Ratcliffe, Norman M. Probert, Chris S. PLoS One Research Article Diagnosing irritable bowel syndrome (IBS) can be a challenge; many clinicians resort to invasive investigations in order to rule out other diseases and reassure their patients. Volatile organic metabolites (VOMs) are emitted from feces; understanding changes in the patterns of these VOMs could aid our understanding of the etiology of the disease and the development of biomarkers, which can assist in the diagnosis of IBS. We report the first comprehensive study of the fecal VOMs patterns in patients with diarrhea-predominant IBS (IBS-D), active Crohn's disease (CD), ulcerative colitis (UC) and healthy controls. 30 patients with IBS-D, 62 with CD, 48 with UC and 109 healthy controls were studied. Diagnosis of IBS-D was made using the Manning criteria and all patients with CD and UC met endoscopic, histologic and/or radiologic criteria. Fecal VOMs were extracted by solid phase microextraction (SPME) and analyzed by gas chromatography-mass spectrometry (GC-MS). 240 VOMs were identified. Univariate analysis showed that esters of short chain fatty acids, cyclohexanecarboxylic acid and its ester derivatives were associated with IBS-D (p<0.05), while aldehydes were more abundant in IBD (p<0.05). A predictive model, developed by multivariate analysis, separated IBS-D from active CD, UC and healthy controls with a sensitivity of 94%, 96% and 90%; and a specificity of 82%, 80% and 80% respectively (p<0.05). The understanding of the derivation of these VOMs may cast light on the etiology of IBS-D and IBD. These data show that fecal VOMs analyses could contribute to the diagnosis of IBS-D, for which there is no laboratory test, as well as IBD. Public Library of Science 2013-03-13 /pmc/articles/PMC3596408/ /pubmed/23516449 http://dx.doi.org/10.1371/journal.pone.0058204 Text en © 2013 Ahmed et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited. |
spellingShingle | Research Article Ahmed, Iftikhar Greenwood, Rosemary Costello, Ben de Lacy Ratcliffe, Norman M. Probert, Chris S. An Investigation of Fecal Volatile Organic Metabolites in Irritable Bowel Syndrome |
title | An Investigation of Fecal Volatile Organic Metabolites in Irritable Bowel Syndrome |
title_full | An Investigation of Fecal Volatile Organic Metabolites in Irritable Bowel Syndrome |
title_fullStr | An Investigation of Fecal Volatile Organic Metabolites in Irritable Bowel Syndrome |
title_full_unstemmed | An Investigation of Fecal Volatile Organic Metabolites in Irritable Bowel Syndrome |
title_short | An Investigation of Fecal Volatile Organic Metabolites in Irritable Bowel Syndrome |
title_sort | investigation of fecal volatile organic metabolites in irritable bowel syndrome |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3596408/ https://www.ncbi.nlm.nih.gov/pubmed/23516449 http://dx.doi.org/10.1371/journal.pone.0058204 |
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