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The preparation and characterization of folate-conjugated human serum albumin magnetic cisplatin nanoparticles()

OBJECTIVE: Nanoparticles are becoming an important method of targeted drug delivery. To evaluate the importance of folate-conjugated human serum albumin (HSA) magnetic nanoparticles (Folate-CDDP/HSA MNP), we prepared drug-loaded Folate-CDDP/HSA MNPs and characterized their features. METHODS: First,...

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Autores principales: Chen, Daozhen, Tang, Qiusha, Xue, Wenqun, Xiang, Jingying, Zhang, Li, Wang, Xinru
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Editorial Department of Journal of Biomedical Research 2010
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3596532/
https://www.ncbi.nlm.nih.gov/pubmed/23554608
http://dx.doi.org/10.1016/S1674-8301(10)60005-X
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author Chen, Daozhen
Tang, Qiusha
Xue, Wenqun
Xiang, Jingying
Zhang, Li
Wang, Xinru
author_facet Chen, Daozhen
Tang, Qiusha
Xue, Wenqun
Xiang, Jingying
Zhang, Li
Wang, Xinru
author_sort Chen, Daozhen
collection PubMed
description OBJECTIVE: Nanoparticles are becoming an important method of targeted drug delivery. To evaluate the importance of folate-conjugated human serum albumin (HSA) magnetic nanoparticles (Folate-CDDP/HSA MNP), we prepared drug-loaded Folate-CDDP/HSA MNPs and characterized their features. METHODS: First, folate was conjugated with HSA under the effect of a condensing agent, and the conjugating rate was evaluated by a colorimetric method using 2, 4, 6 - trinitrobenzene sulfonic acid. Second, under N(2) gas, Fe(3)O(4) magnetic nanomaterials were prepared and characterized by using transmission electron microscopy (TEM), SEM-EDS and X-ray diffraction (XRD). Finally, Folate-CDDP/HSA MNP was prepared by using a solvent evaporation technique. TEM was used to observe particle morphology. The particle size and distribution of the prepared complexes were determined by a Laser particle size analyzer. Drug loading volume and drug release were investigated by a high performance liquid chromatography method (HPLC) in vitro. RESULTS: We successfully prepared folate-conjugated HSA and its conjugating rate was 27.26 µg/mg. Under TEM, Fe(3)O(4) magnetic nanoparticles were highly electron density and had an even size distribution in the range of 10-20 nm. It was confirmed by SEM-EDS and XRD that Fe(3)O(4) magnetic nanoparticles had been successfully prepared. Under TEM, drug-loaded magnetic nanoparticles were observed, which had a round shape, similar uniform size and smooth surface. Their average size was 79 nm which was determined by laser scattering, and they exhibited magnetic responsiveness. Encapsulation efficiency was 89.75% and effective drug loading was calculated to be 15.25%. The release results in vitro showed that the half release time (t½) of cisplatin in cisplatin Solution and Folate-CDDP/HSA MNP was 65 min and 24 h respectively, which indicated that microspheres had an obvious effect of sustained-release. CONCLUSION: Folate-CDDP/HSA MNPs were prepared successfully. The preparation process and related characteristics data provided a foundation for further study, including the mechanism of the nanoparticles distribution in vivo and their intake by tumor cells.
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spelling pubmed-35965322013-04-02 The preparation and characterization of folate-conjugated human serum albumin magnetic cisplatin nanoparticles() Chen, Daozhen Tang, Qiusha Xue, Wenqun Xiang, Jingying Zhang, Li Wang, Xinru J Biomed Res Research Paper OBJECTIVE: Nanoparticles are becoming an important method of targeted drug delivery. To evaluate the importance of folate-conjugated human serum albumin (HSA) magnetic nanoparticles (Folate-CDDP/HSA MNP), we prepared drug-loaded Folate-CDDP/HSA MNPs and characterized their features. METHODS: First, folate was conjugated with HSA under the effect of a condensing agent, and the conjugating rate was evaluated by a colorimetric method using 2, 4, 6 - trinitrobenzene sulfonic acid. Second, under N(2) gas, Fe(3)O(4) magnetic nanomaterials were prepared and characterized by using transmission electron microscopy (TEM), SEM-EDS and X-ray diffraction (XRD). Finally, Folate-CDDP/HSA MNP was prepared by using a solvent evaporation technique. TEM was used to observe particle morphology. The particle size and distribution of the prepared complexes were determined by a Laser particle size analyzer. Drug loading volume and drug release were investigated by a high performance liquid chromatography method (HPLC) in vitro. RESULTS: We successfully prepared folate-conjugated HSA and its conjugating rate was 27.26 µg/mg. Under TEM, Fe(3)O(4) magnetic nanoparticles were highly electron density and had an even size distribution in the range of 10-20 nm. It was confirmed by SEM-EDS and XRD that Fe(3)O(4) magnetic nanoparticles had been successfully prepared. Under TEM, drug-loaded magnetic nanoparticles were observed, which had a round shape, similar uniform size and smooth surface. Their average size was 79 nm which was determined by laser scattering, and they exhibited magnetic responsiveness. Encapsulation efficiency was 89.75% and effective drug loading was calculated to be 15.25%. The release results in vitro showed that the half release time (t½) of cisplatin in cisplatin Solution and Folate-CDDP/HSA MNP was 65 min and 24 h respectively, which indicated that microspheres had an obvious effect of sustained-release. CONCLUSION: Folate-CDDP/HSA MNPs were prepared successfully. The preparation process and related characteristics data provided a foundation for further study, including the mechanism of the nanoparticles distribution in vivo and their intake by tumor cells. Editorial Department of Journal of Biomedical Research 2010-01 /pmc/articles/PMC3596532/ /pubmed/23554608 http://dx.doi.org/10.1016/S1674-8301(10)60005-X Text en © 2010 by the Journal of Biomedical Research. All rights reserved. This work is licensed under a Creative Commons Attribution 3.0 Unported License. To view a copy of this license, visit http://creativecommons.org/licenses/by/3.0/
spellingShingle Research Paper
Chen, Daozhen
Tang, Qiusha
Xue, Wenqun
Xiang, Jingying
Zhang, Li
Wang, Xinru
The preparation and characterization of folate-conjugated human serum albumin magnetic cisplatin nanoparticles()
title The preparation and characterization of folate-conjugated human serum albumin magnetic cisplatin nanoparticles()
title_full The preparation and characterization of folate-conjugated human serum albumin magnetic cisplatin nanoparticles()
title_fullStr The preparation and characterization of folate-conjugated human serum albumin magnetic cisplatin nanoparticles()
title_full_unstemmed The preparation and characterization of folate-conjugated human serum albumin magnetic cisplatin nanoparticles()
title_short The preparation and characterization of folate-conjugated human serum albumin magnetic cisplatin nanoparticles()
title_sort preparation and characterization of folate-conjugated human serum albumin magnetic cisplatin nanoparticles()
topic Research Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3596532/
https://www.ncbi.nlm.nih.gov/pubmed/23554608
http://dx.doi.org/10.1016/S1674-8301(10)60005-X
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