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A novel SATB1 binding site in the BCL2 promoter region possesses transcriptional regulatory function()
BCL2 is a key regulator of apoptosis. Our previous work has demonstrated that special AT-rich sequence-binding protein 1 (SATB1) is positively correlated with BCL2 expression. In the present study, we report a new SATB1 binding site located between P1 and P2 promoters of the BCL2 gene. The candidate...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Editorial Department of Journal of Biomedical Research
2010
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3596693/ https://www.ncbi.nlm.nih.gov/pubmed/23554662 http://dx.doi.org/10.1016/S1674-8301(10)60060-7 |
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author | Gong, Feiran Sun, Luan Sun, Yujie |
author_facet | Gong, Feiran Sun, Luan Sun, Yujie |
author_sort | Gong, Feiran |
collection | PubMed |
description | BCL2 is a key regulator of apoptosis. Our previous work has demonstrated that special AT-rich sequence-binding protein 1 (SATB1) is positively correlated with BCL2 expression. In the present study, we report a new SATB1 binding site located between P1 and P2 promoters of the BCL2 gene. The candidate SATB1 binding sequence predicted by bioinformatic analysis was investigated in vitro and in vivo by electrophoretic gel mobility shift assays (EMSA) and chromatin immunoprecipitation (ChIP). One 25-bp sequence, named SB1, was confirmed to be SATB1 binding site. The regulatory function of SB1 and its relevance to SATB1 were further examed with dual-luciferase reporter assay system in Jurkat cells. We found that SB1 could negatively regulate reporter gene activity. Mutation of SATB1 binding site further repressed the activity. Knockdown of SATB1 also enhanced this negative effect of SB1. Our data indicate that the SB1 sequence possesses negative transcriptional regulatory function and this function can be antagonized by SATB1. |
format | Online Article Text |
id | pubmed-3596693 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2010 |
publisher | Editorial Department of Journal of Biomedical Research |
record_format | MEDLINE/PubMed |
spelling | pubmed-35966932013-04-02 A novel SATB1 binding site in the BCL2 promoter region possesses transcriptional regulatory function() Gong, Feiran Sun, Luan Sun, Yujie J Biomed Res Research Paper BCL2 is a key regulator of apoptosis. Our previous work has demonstrated that special AT-rich sequence-binding protein 1 (SATB1) is positively correlated with BCL2 expression. In the present study, we report a new SATB1 binding site located between P1 and P2 promoters of the BCL2 gene. The candidate SATB1 binding sequence predicted by bioinformatic analysis was investigated in vitro and in vivo by electrophoretic gel mobility shift assays (EMSA) and chromatin immunoprecipitation (ChIP). One 25-bp sequence, named SB1, was confirmed to be SATB1 binding site. The regulatory function of SB1 and its relevance to SATB1 were further examed with dual-luciferase reporter assay system in Jurkat cells. We found that SB1 could negatively regulate reporter gene activity. Mutation of SATB1 binding site further repressed the activity. Knockdown of SATB1 also enhanced this negative effect of SB1. Our data indicate that the SB1 sequence possesses negative transcriptional regulatory function and this function can be antagonized by SATB1. Editorial Department of Journal of Biomedical Research 2010-11 /pmc/articles/PMC3596693/ /pubmed/23554662 http://dx.doi.org/10.1016/S1674-8301(10)60060-7 Text en © 2010 by the Journal of Biomedical Research. All rights reserved. This work is licensed under a Creative Commons Attribution 3.0 Unported License. To view a copy of this license, visit http://creativecommons.org/licenses/by/3.0/ |
spellingShingle | Research Paper Gong, Feiran Sun, Luan Sun, Yujie A novel SATB1 binding site in the BCL2 promoter region possesses transcriptional regulatory function() |
title | A novel SATB1 binding site in the BCL2 promoter region possesses transcriptional regulatory function() |
title_full | A novel SATB1 binding site in the BCL2 promoter region possesses transcriptional regulatory function() |
title_fullStr | A novel SATB1 binding site in the BCL2 promoter region possesses transcriptional regulatory function() |
title_full_unstemmed | A novel SATB1 binding site in the BCL2 promoter region possesses transcriptional regulatory function() |
title_short | A novel SATB1 binding site in the BCL2 promoter region possesses transcriptional regulatory function() |
title_sort | novel satb1 binding site in the bcl2 promoter region possesses transcriptional regulatory function() |
topic | Research Paper |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3596693/ https://www.ncbi.nlm.nih.gov/pubmed/23554662 http://dx.doi.org/10.1016/S1674-8301(10)60060-7 |
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