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Comparison of liquid-liquid extraction-thin layer chromatography with solid-phase extraction-high-performance thin layer chromatography in detection of urinary morphine
Liquid-liquid extraction-thin layer chromatography (LLE-TLC) has been a common and routine combined method for detection of drugs in biological materials. Solid-phase extraction (SPE) is gradually replacing the traditional LLE method. High performance thin layer chromatography (HPTLC) has several ad...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Editorial Department of Journal of Biomedical Research
2011
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3596733/ https://www.ncbi.nlm.nih.gov/pubmed/23554712 http://dx.doi.org/10.1016/S1674-8301(11)60048-1 |
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author | Ahadi, Ali Partoazar, Alireza Abedi-Khorasgani, Mohammad-Hassan Shetab-Boushehri, Seyed Vahid |
author_facet | Ahadi, Ali Partoazar, Alireza Abedi-Khorasgani, Mohammad-Hassan Shetab-Boushehri, Seyed Vahid |
author_sort | Ahadi, Ali |
collection | PubMed |
description | Liquid-liquid extraction-thin layer chromatography (LLE-TLC) has been a common and routine combined method for detection of drugs in biological materials. Solid-phase extraction (SPE) is gradually replacing the traditional LLE method. High performance thin layer chromatography (HPTLC) has several advantages over TLC. The present work studied the higher efficiency of a new SPE-HPTLC method over that of a routine LLE-TLC method, in extraction and detection of urinary morphine. Fifty-eight urine samples, primarily identified as morphine-positive samples by a strip test, were re-screened by LLE-TLC and SPE-HPTLC. The results of LLE-TLC and SPE-HPTLC were then compared with each other. The results showed that the SPE-HPTLC detected 74% of total samples as morphine-positive samples whereas the LLE-TLC detected 48% of the same samples. We further discussed the effect of codeine abuse on TLC analysis of urinary morphine. Regarding the importance of morphine detection in urine, the present combined SPE-HPTLC method is suggested as a replacement method for detection of urinary morphine by many reference laboratories. |
format | Online Article Text |
id | pubmed-3596733 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2011 |
publisher | Editorial Department of Journal of Biomedical Research |
record_format | MEDLINE/PubMed |
spelling | pubmed-35967332013-04-02 Comparison of liquid-liquid extraction-thin layer chromatography with solid-phase extraction-high-performance thin layer chromatography in detection of urinary morphine Ahadi, Ali Partoazar, Alireza Abedi-Khorasgani, Mohammad-Hassan Shetab-Boushehri, Seyed Vahid J Biomed Res Research Paper Liquid-liquid extraction-thin layer chromatography (LLE-TLC) has been a common and routine combined method for detection of drugs in biological materials. Solid-phase extraction (SPE) is gradually replacing the traditional LLE method. High performance thin layer chromatography (HPTLC) has several advantages over TLC. The present work studied the higher efficiency of a new SPE-HPTLC method over that of a routine LLE-TLC method, in extraction and detection of urinary morphine. Fifty-eight urine samples, primarily identified as morphine-positive samples by a strip test, were re-screened by LLE-TLC and SPE-HPTLC. The results of LLE-TLC and SPE-HPTLC were then compared with each other. The results showed that the SPE-HPTLC detected 74% of total samples as morphine-positive samples whereas the LLE-TLC detected 48% of the same samples. We further discussed the effect of codeine abuse on TLC analysis of urinary morphine. Regarding the importance of morphine detection in urine, the present combined SPE-HPTLC method is suggested as a replacement method for detection of urinary morphine by many reference laboratories. Editorial Department of Journal of Biomedical Research 2011-09 /pmc/articles/PMC3596733/ /pubmed/23554712 http://dx.doi.org/10.1016/S1674-8301(11)60048-1 Text en © 2011 by the Journal of Biomedical Research. All rights reserved. This work is licensed under a Creative Commons Attribution 3.0 Unported License. To view a copy of this license, visit http://creativecommons.org/licenses/by/3.0/ |
spellingShingle | Research Paper Ahadi, Ali Partoazar, Alireza Abedi-Khorasgani, Mohammad-Hassan Shetab-Boushehri, Seyed Vahid Comparison of liquid-liquid extraction-thin layer chromatography with solid-phase extraction-high-performance thin layer chromatography in detection of urinary morphine |
title | Comparison of liquid-liquid extraction-thin layer chromatography with solid-phase extraction-high-performance thin layer chromatography in detection of urinary morphine |
title_full | Comparison of liquid-liquid extraction-thin layer chromatography with solid-phase extraction-high-performance thin layer chromatography in detection of urinary morphine |
title_fullStr | Comparison of liquid-liquid extraction-thin layer chromatography with solid-phase extraction-high-performance thin layer chromatography in detection of urinary morphine |
title_full_unstemmed | Comparison of liquid-liquid extraction-thin layer chromatography with solid-phase extraction-high-performance thin layer chromatography in detection of urinary morphine |
title_short | Comparison of liquid-liquid extraction-thin layer chromatography with solid-phase extraction-high-performance thin layer chromatography in detection of urinary morphine |
title_sort | comparison of liquid-liquid extraction-thin layer chromatography with solid-phase extraction-high-performance thin layer chromatography in detection of urinary morphine |
topic | Research Paper |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3596733/ https://www.ncbi.nlm.nih.gov/pubmed/23554712 http://dx.doi.org/10.1016/S1674-8301(11)60048-1 |
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