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All-trans retinoic acid upregulates VEGF expression in glioma cells in vitro

All-trans retinoid acid (ATRA) is one of the most potent and most thoroughly studied differentiation inducers that induce the differentiation and apoptosis of glioma cells. However, the effect of ATRA on angiogenesis of glioma remains poorly understood. We examined the effect of ATRA on the expressi...

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Detalles Bibliográficos
Autores principales: Liang, Chen, Guo, Shiwen, Yang, Ling
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Editorial Department of Journal of Biomedical Research 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3596755/
https://www.ncbi.nlm.nih.gov/pubmed/23554794
http://dx.doi.org/10.7555/JBR.27.20120048
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author Liang, Chen
Guo, Shiwen
Yang, Ling
author_facet Liang, Chen
Guo, Shiwen
Yang, Ling
author_sort Liang, Chen
collection PubMed
description All-trans retinoid acid (ATRA) is one of the most potent and most thoroughly studied differentiation inducers that induce the differentiation and apoptosis of glioma cells. However, the effect of ATRA on angiogenesis of glioma remains poorly understood. We examined the effect of ATRA on the expression of vascular endothelial growth factor (VEGF) in different glioma cell lines and investigated the underlying mechanism, intending to partially reveal the effects of ATRA on angiogenesis of glioma. Glioma cells were treated by ATRA at 5 and 10 µmol/L. The VEGF mRNA transcript levels were determined by real-time RT-PCR and the protein levels of VEGF in glioma cells were evaluated by Western blotting assays. Moreover, hypoxia-inducible factor-1α (HIF-1α) mRNA expression was analyzed by using real-time RT-PCR. After treatment with 5 and 10 µmol/L ATRA, the VEGF mRNA transcript levels in glioma cells increased remarkably, compared with that in the control group, and the relative protein expression of VEGF was also up-regulated. Meanwhile, the HIF-1α mRNA expression also increased. ATRA increases the expression of VEGF in glioma cells at both transcriptional and translational levels.
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spelling pubmed-35967552013-04-02 All-trans retinoic acid upregulates VEGF expression in glioma cells in vitro Liang, Chen Guo, Shiwen Yang, Ling J Biomed Res Research Paper All-trans retinoid acid (ATRA) is one of the most potent and most thoroughly studied differentiation inducers that induce the differentiation and apoptosis of glioma cells. However, the effect of ATRA on angiogenesis of glioma remains poorly understood. We examined the effect of ATRA on the expression of vascular endothelial growth factor (VEGF) in different glioma cell lines and investigated the underlying mechanism, intending to partially reveal the effects of ATRA on angiogenesis of glioma. Glioma cells were treated by ATRA at 5 and 10 µmol/L. The VEGF mRNA transcript levels were determined by real-time RT-PCR and the protein levels of VEGF in glioma cells were evaluated by Western blotting assays. Moreover, hypoxia-inducible factor-1α (HIF-1α) mRNA expression was analyzed by using real-time RT-PCR. After treatment with 5 and 10 µmol/L ATRA, the VEGF mRNA transcript levels in glioma cells increased remarkably, compared with that in the control group, and the relative protein expression of VEGF was also up-regulated. Meanwhile, the HIF-1α mRNA expression also increased. ATRA increases the expression of VEGF in glioma cells at both transcriptional and translational levels. Editorial Department of Journal of Biomedical Research 2013-01 2012-12-19 /pmc/articles/PMC3596755/ /pubmed/23554794 http://dx.doi.org/10.7555/JBR.27.20120048 Text en © 2013 by the Journal of Biomedical Research. All rights reserved. This work is licensed under a Creative Commons Attribution 3.0 Unported License. To view a copy of this license, visit http://creativecommons.org/licenses/by/3.0/
spellingShingle Research Paper
Liang, Chen
Guo, Shiwen
Yang, Ling
All-trans retinoic acid upregulates VEGF expression in glioma cells in vitro
title All-trans retinoic acid upregulates VEGF expression in glioma cells in vitro
title_full All-trans retinoic acid upregulates VEGF expression in glioma cells in vitro
title_fullStr All-trans retinoic acid upregulates VEGF expression in glioma cells in vitro
title_full_unstemmed All-trans retinoic acid upregulates VEGF expression in glioma cells in vitro
title_short All-trans retinoic acid upregulates VEGF expression in glioma cells in vitro
title_sort all-trans retinoic acid upregulates vegf expression in glioma cells in vitro
topic Research Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3596755/
https://www.ncbi.nlm.nih.gov/pubmed/23554794
http://dx.doi.org/10.7555/JBR.27.20120048
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