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Localization of Methyl-Coenzyme M Reductase as Metabolic Marker for Diverse Methanogenic Archaea
Methyl-Coenzyme M reductase (MCR) as key enzyme for methanogenesis as well as for anaerobic oxidation of methane represents an important metabolic marker for both processes in microbial biofilms. Here, the potential of MCR-specific polyclonal antibodies as metabolic marker in various methanogenic Ar...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Hindawi Publishing Corporation
2013
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3596918/ https://www.ncbi.nlm.nih.gov/pubmed/23533332 http://dx.doi.org/10.1155/2013/920241 |
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author | Wrede, Christoph Walbaum, Ulrike Ducki, Andrea Heieren, Iris Hoppert, Michael |
author_facet | Wrede, Christoph Walbaum, Ulrike Ducki, Andrea Heieren, Iris Hoppert, Michael |
author_sort | Wrede, Christoph |
collection | PubMed |
description | Methyl-Coenzyme M reductase (MCR) as key enzyme for methanogenesis as well as for anaerobic oxidation of methane represents an important metabolic marker for both processes in microbial biofilms. Here, the potential of MCR-specific polyclonal antibodies as metabolic marker in various methanogenic Archaea is shown. For standard growth conditions in laboratory culture, the cytoplasmic localization of the enzyme in Methanothermobacter marburgensis, Methanothermobacter wolfei, Methanococcus maripaludis, Methanosarcina mazei, and in anaerobically methane-oxidizing biofilms is demonstrated. Under growth limiting conditions on nickel-depleted media, at low linear growth of cultures, a fraction of 50–70% of the enzyme was localized close to the cytoplasmic membrane, which implies “facultative” membrane association of the enzyme. This feature may be also useful for assessment of growth-limiting conditions in microbial biofilms. |
format | Online Article Text |
id | pubmed-3596918 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2013 |
publisher | Hindawi Publishing Corporation |
record_format | MEDLINE/PubMed |
spelling | pubmed-35969182013-03-26 Localization of Methyl-Coenzyme M Reductase as Metabolic Marker for Diverse Methanogenic Archaea Wrede, Christoph Walbaum, Ulrike Ducki, Andrea Heieren, Iris Hoppert, Michael Archaea Research Article Methyl-Coenzyme M reductase (MCR) as key enzyme for methanogenesis as well as for anaerobic oxidation of methane represents an important metabolic marker for both processes in microbial biofilms. Here, the potential of MCR-specific polyclonal antibodies as metabolic marker in various methanogenic Archaea is shown. For standard growth conditions in laboratory culture, the cytoplasmic localization of the enzyme in Methanothermobacter marburgensis, Methanothermobacter wolfei, Methanococcus maripaludis, Methanosarcina mazei, and in anaerobically methane-oxidizing biofilms is demonstrated. Under growth limiting conditions on nickel-depleted media, at low linear growth of cultures, a fraction of 50–70% of the enzyme was localized close to the cytoplasmic membrane, which implies “facultative” membrane association of the enzyme. This feature may be also useful for assessment of growth-limiting conditions in microbial biofilms. Hindawi Publishing Corporation 2013-02-25 /pmc/articles/PMC3596918/ /pubmed/23533332 http://dx.doi.org/10.1155/2013/920241 Text en Copyright © 2013 Christoph Wrede et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Wrede, Christoph Walbaum, Ulrike Ducki, Andrea Heieren, Iris Hoppert, Michael Localization of Methyl-Coenzyme M Reductase as Metabolic Marker for Diverse Methanogenic Archaea |
title | Localization of Methyl-Coenzyme M Reductase as Metabolic Marker for Diverse Methanogenic Archaea |
title_full | Localization of Methyl-Coenzyme M Reductase as Metabolic Marker for Diverse Methanogenic Archaea |
title_fullStr | Localization of Methyl-Coenzyme M Reductase as Metabolic Marker for Diverse Methanogenic Archaea |
title_full_unstemmed | Localization of Methyl-Coenzyme M Reductase as Metabolic Marker for Diverse Methanogenic Archaea |
title_short | Localization of Methyl-Coenzyme M Reductase as Metabolic Marker for Diverse Methanogenic Archaea |
title_sort | localization of methyl-coenzyme m reductase as metabolic marker for diverse methanogenic archaea |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3596918/ https://www.ncbi.nlm.nih.gov/pubmed/23533332 http://dx.doi.org/10.1155/2013/920241 |
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