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Imaging Live Bee Brains using Minimally-Invasive Diagnostic Radioentomology
The sensitivity of the honey bee, Apis mellifera L. (Hymeonoptera: Apidae), brain volume and density to behavior (plasticity) makes it a great model for exploring the interactions between experience, behavior, and brain structure. Plasticity in the adult bee brain has been demonstrated in previous e...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
University of Wisconsin Library
2012
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3596940/ https://www.ncbi.nlm.nih.gov/pubmed/23421752 http://dx.doi.org/10.1673/031.012.8901 |
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author | Greco, Mark K Tong, Jenna Soleimani, Manucher Bell, Duncan Schäfer, Marc O |
author_facet | Greco, Mark K Tong, Jenna Soleimani, Manucher Bell, Duncan Schäfer, Marc O |
author_sort | Greco, Mark K |
collection | PubMed |
description | The sensitivity of the honey bee, Apis mellifera L. (Hymeonoptera: Apidae), brain volume and density to behavior (plasticity) makes it a great model for exploring the interactions between experience, behavior, and brain structure. Plasticity in the adult bee brain has been demonstrated in previous experiments. This experiment was conducted to identify the potentials and limitations of MicroCT (micro computed tomograpy) scanning “live” bees as a more comprehensive, non-invasive method for brain morphology and physiology. Bench-top and synchrotron MicroCT were used to scan live bees. For improved tissue differentiation, bees were fed and injected with radiographic contrast. Images of optic lobes, ocelli, antennal lobes, and mushroom bodies were visualized in 2D and 3D rendering modes. Scanning of live bees (for the first time) enabled minimally-invasive imaging of physiological processes such as passage of contrast from gut to haemolymph, and preliminary brain perfusion studies. The use of microCT scanning for studying insects (collectively termed ‘diagnostic radioentomology’, or DR) is increasing. Our results indicate that it is feasible to observe plasticity of the honey bee brain in vivo using diagnostic radioentomology, and that progressive, real-time observations of these changes can be followed in individual live bees. Limitations of live bee scanning, such as movement errors and poor tissue differentiation, were identified; however, there is great potential for in-vivo, non-invasive diagnostic radioentomology imaging of the honey bee for brain morphology and physiology. |
format | Online Article Text |
id | pubmed-3596940 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2012 |
publisher | University of Wisconsin Library |
record_format | MEDLINE/PubMed |
spelling | pubmed-35969402013-03-15 Imaging Live Bee Brains using Minimally-Invasive Diagnostic Radioentomology Greco, Mark K Tong, Jenna Soleimani, Manucher Bell, Duncan Schäfer, Marc O J Insect Sci Article The sensitivity of the honey bee, Apis mellifera L. (Hymeonoptera: Apidae), brain volume and density to behavior (plasticity) makes it a great model for exploring the interactions between experience, behavior, and brain structure. Plasticity in the adult bee brain has been demonstrated in previous experiments. This experiment was conducted to identify the potentials and limitations of MicroCT (micro computed tomograpy) scanning “live” bees as a more comprehensive, non-invasive method for brain morphology and physiology. Bench-top and synchrotron MicroCT were used to scan live bees. For improved tissue differentiation, bees were fed and injected with radiographic contrast. Images of optic lobes, ocelli, antennal lobes, and mushroom bodies were visualized in 2D and 3D rendering modes. Scanning of live bees (for the first time) enabled minimally-invasive imaging of physiological processes such as passage of contrast from gut to haemolymph, and preliminary brain perfusion studies. The use of microCT scanning for studying insects (collectively termed ‘diagnostic radioentomology’, or DR) is increasing. Our results indicate that it is feasible to observe plasticity of the honey bee brain in vivo using diagnostic radioentomology, and that progressive, real-time observations of these changes can be followed in individual live bees. Limitations of live bee scanning, such as movement errors and poor tissue differentiation, were identified; however, there is great potential for in-vivo, non-invasive diagnostic radioentomology imaging of the honey bee for brain morphology and physiology. University of Wisconsin Library 2012-07-27 /pmc/articles/PMC3596940/ /pubmed/23421752 http://dx.doi.org/10.1673/031.012.8901 Text en © 2012 http://creativecommons.org/licenses/by/2.5/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Article Greco, Mark K Tong, Jenna Soleimani, Manucher Bell, Duncan Schäfer, Marc O Imaging Live Bee Brains using Minimally-Invasive Diagnostic Radioentomology |
title | Imaging Live Bee Brains using Minimally-Invasive Diagnostic Radioentomology |
title_full | Imaging Live Bee Brains using Minimally-Invasive Diagnostic Radioentomology |
title_fullStr | Imaging Live Bee Brains using Minimally-Invasive Diagnostic Radioentomology |
title_full_unstemmed | Imaging Live Bee Brains using Minimally-Invasive Diagnostic Radioentomology |
title_short | Imaging Live Bee Brains using Minimally-Invasive Diagnostic Radioentomology |
title_sort | imaging live bee brains using minimally-invasive diagnostic radioentomology |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3596940/ https://www.ncbi.nlm.nih.gov/pubmed/23421752 http://dx.doi.org/10.1673/031.012.8901 |
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