Effect of transient expression of fluorescent protein probes in synovial and myoblast cell lines

Here, we investigate the appropriate fluorescent proteins for use in the culture of synovial MH7A and myoblast C2C12 cells. Fluorescent signal intensities of 3 different fluorescent proteins were examined in these cell lines. The fluorescent intensity of transiently expressed AcGFP, DsRed, and mStra...

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Detalles Bibliográficos
Autores principales: Shibasaki, Seiji, Fujita, Aika, Usui, Chihiro, Watanabe, Sachiko, Kitano, Sachie, Sano, Hajime, Iwasaki, Tsuyoshi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer International Publishing AG 2012
Materias:
Research
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3597265/
https://www.ncbi.nlm.nih.gov/pubmed/23503703
http://dx.doi.org/10.1186/2193-1801-1-36
Descripción
Sumario:Here, we investigate the appropriate fluorescent proteins for use in the culture of synovial MH7A and myoblast C2C12 cells. Fluorescent signal intensities of 3 different fluorescent proteins were examined in these cell lines. The fluorescent intensity of transiently expressed AcGFP, DsRed, and mStrawberry were examined in these cell lines, and the influence of the amount of plasmid used on transfection efficiency and cell viability were investigated.

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