Effect of transient expression of fluorescent protein probes in synovial and myoblast cell lines

Here, we investigate the appropriate fluorescent proteins for use in the culture of synovial MH7A and myoblast C2C12 cells. Fluorescent signal intensities of 3 different fluorescent proteins were examined in these cell lines. The fluorescent intensity of transiently expressed AcGFP, DsRed, and mStra...

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Autores principales: Shibasaki, Seiji, Fujita, Aika, Usui, Chihiro, Watanabe, Sachiko, Kitano, Sachie, Sano, Hajime, Iwasaki, Tsuyoshi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer International Publishing AG 2012
Materias:
Research
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3597265/
https://www.ncbi.nlm.nih.gov/pubmed/23503703
http://dx.doi.org/10.1186/2193-1801-1-36
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author Shibasaki, Seiji
Fujita, Aika
Usui, Chihiro
Watanabe, Sachiko
Kitano, Sachie
Sano, Hajime
Iwasaki, Tsuyoshi
author_facet Shibasaki, Seiji
Fujita, Aika
Usui, Chihiro
Watanabe, Sachiko
Kitano, Sachie
Sano, Hajime
Iwasaki, Tsuyoshi
author_sort Shibasaki, Seiji
collection PubMed
description Here, we investigate the appropriate fluorescent proteins for use in the culture of synovial MH7A and myoblast C2C12 cells. Fluorescent signal intensities of 3 different fluorescent proteins were examined in these cell lines. The fluorescent intensity of transiently expressed AcGFP, DsRed, and mStrawberry were examined in these cell lines, and the influence of the amount of plasmid used on transfection efficiency and cell viability were investigated.
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spelling pubmed-35972652013-03-15 Effect of transient expression of fluorescent protein probes in synovial and myoblast cell lines Shibasaki, Seiji Fujita, Aika Usui, Chihiro Watanabe, Sachiko Kitano, Sachie Sano, Hajime Iwasaki, Tsuyoshi Springerplus Research Here, we investigate the appropriate fluorescent proteins for use in the culture of synovial MH7A and myoblast C2C12 cells. Fluorescent signal intensities of 3 different fluorescent proteins were examined in these cell lines. The fluorescent intensity of transiently expressed AcGFP, DsRed, and mStrawberry were examined in these cell lines, and the influence of the amount of plasmid used on transfection efficiency and cell viability were investigated. Springer International Publishing AG 2012-10-22 /pmc/articles/PMC3597265/ /pubmed/23503703 http://dx.doi.org/10.1186/2193-1801-1-36 Text en © Shibasaki et al; licensee Springer. 2012 This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research
Shibasaki, Seiji
Fujita, Aika
Usui, Chihiro
Watanabe, Sachiko
Kitano, Sachie
Sano, Hajime
Iwasaki, Tsuyoshi
Effect of transient expression of fluorescent protein probes in synovial and myoblast cell lines
title Effect of transient expression of fluorescent protein probes in synovial and myoblast cell lines
title_full Effect of transient expression of fluorescent protein probes in synovial and myoblast cell lines
title_fullStr Effect of transient expression of fluorescent protein probes in synovial and myoblast cell lines
title_full_unstemmed Effect of transient expression of fluorescent protein probes in synovial and myoblast cell lines
title_short Effect of transient expression of fluorescent protein probes in synovial and myoblast cell lines
title_sort effect of transient expression of fluorescent protein probes in synovial and myoblast cell lines
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3597265/
https://www.ncbi.nlm.nih.gov/pubmed/23503703
http://dx.doi.org/10.1186/2193-1801-1-36
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