Reflection-mode TERS on Insulin Amyloid Fibrils with Top-Visual AFM Probes

Tip-enhanced Raman spectroscopy provides chemical information while raster scanning samples with topographical detail. The coupling of atomic force microscopy and Raman spectroscopy in top illumination optical setup is a powerful configuration to resolve nanometer structures while collecting reflect...

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Detalles Bibliográficos
Autores principales: Moretti, Manola, Proietti Zaccaria, Remo, Descrovi, Emiliano, Das, Gobind, Leoncini, Marco, Liberale, Carlo, De Angelis, Francesco, Di Fabrizio, Enzo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer US 2012
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3597279/
https://www.ncbi.nlm.nih.gov/pubmed/23504187
http://dx.doi.org/10.1007/s11468-012-9385-x
Descripción
Sumario:Tip-enhanced Raman spectroscopy provides chemical information while raster scanning samples with topographical detail. The coupling of atomic force microscopy and Raman spectroscopy in top illumination optical setup is a powerful configuration to resolve nanometer structures while collecting reflection mode backscattered signal. Here, we theoretically calculate the field enhancement generated by TER spectroscopy with top illumination geometry and we apply the technique to the characterization of insulin amyloid fibrils. We experimentally confirm that this technique is able to enhance the Raman signal of the polypeptide chain by a factor of 10(5), thus revealing details down to few molecules resolution.

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