Reflection-mode TERS on Insulin Amyloid Fibrils with Top-Visual AFM Probes

Tip-enhanced Raman spectroscopy provides chemical information while raster scanning samples with topographical detail. The coupling of atomic force microscopy and Raman spectroscopy in top illumination optical setup is a powerful configuration to resolve nanometer structures while collecting reflect...

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Detalles Bibliográficos
Autores principales: Moretti, Manola, Proietti Zaccaria, Remo, Descrovi, Emiliano, Das, Gobind, Leoncini, Marco, Liberale, Carlo, De Angelis, Francesco, Di Fabrizio, Enzo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer US 2012
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3597279/
https://www.ncbi.nlm.nih.gov/pubmed/23504187
http://dx.doi.org/10.1007/s11468-012-9385-x
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author Moretti, Manola
Proietti Zaccaria, Remo
Descrovi, Emiliano
Das, Gobind
Leoncini, Marco
Liberale, Carlo
De Angelis, Francesco
Di Fabrizio, Enzo
author_facet Moretti, Manola
Proietti Zaccaria, Remo
Descrovi, Emiliano
Das, Gobind
Leoncini, Marco
Liberale, Carlo
De Angelis, Francesco
Di Fabrizio, Enzo
author_sort Moretti, Manola
collection PubMed
description Tip-enhanced Raman spectroscopy provides chemical information while raster scanning samples with topographical detail. The coupling of atomic force microscopy and Raman spectroscopy in top illumination optical setup is a powerful configuration to resolve nanometer structures while collecting reflection mode backscattered signal. Here, we theoretically calculate the field enhancement generated by TER spectroscopy with top illumination geometry and we apply the technique to the characterization of insulin amyloid fibrils. We experimentally confirm that this technique is able to enhance the Raman signal of the polypeptide chain by a factor of 10(5), thus revealing details down to few molecules resolution.
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spelling pubmed-35972792013-03-15 Reflection-mode TERS on Insulin Amyloid Fibrils with Top-Visual AFM Probes Moretti, Manola Proietti Zaccaria, Remo Descrovi, Emiliano Das, Gobind Leoncini, Marco Liberale, Carlo De Angelis, Francesco Di Fabrizio, Enzo Plasmonics Article Tip-enhanced Raman spectroscopy provides chemical information while raster scanning samples with topographical detail. The coupling of atomic force microscopy and Raman spectroscopy in top illumination optical setup is a powerful configuration to resolve nanometer structures while collecting reflection mode backscattered signal. Here, we theoretically calculate the field enhancement generated by TER spectroscopy with top illumination geometry and we apply the technique to the characterization of insulin amyloid fibrils. We experimentally confirm that this technique is able to enhance the Raman signal of the polypeptide chain by a factor of 10(5), thus revealing details down to few molecules resolution. Springer US 2012-06-15 2013 /pmc/articles/PMC3597279/ /pubmed/23504187 http://dx.doi.org/10.1007/s11468-012-9385-x Text en © The Author(s) 2012 https://creativecommons.org/licenses/by/4.0/ This article is distributed under the terms of the Creative Commons Attribution License which permits any use, distribution, and reproduction in any medium, provided the original author(s) and the source are credited.
spellingShingle Article
Moretti, Manola
Proietti Zaccaria, Remo
Descrovi, Emiliano
Das, Gobind
Leoncini, Marco
Liberale, Carlo
De Angelis, Francesco
Di Fabrizio, Enzo
Reflection-mode TERS on Insulin Amyloid Fibrils with Top-Visual AFM Probes
title Reflection-mode TERS on Insulin Amyloid Fibrils with Top-Visual AFM Probes
title_full Reflection-mode TERS on Insulin Amyloid Fibrils with Top-Visual AFM Probes
title_fullStr Reflection-mode TERS on Insulin Amyloid Fibrils with Top-Visual AFM Probes
title_full_unstemmed Reflection-mode TERS on Insulin Amyloid Fibrils with Top-Visual AFM Probes
title_short Reflection-mode TERS on Insulin Amyloid Fibrils with Top-Visual AFM Probes
title_sort reflection-mode ters on insulin amyloid fibrils with top-visual afm probes
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3597279/
https://www.ncbi.nlm.nih.gov/pubmed/23504187
http://dx.doi.org/10.1007/s11468-012-9385-x
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