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Conjunctival MicroRNA Expression in Inflammatory Trachomatous Scarring

PURPOSE: Trachoma is a fibrotic disease of the conjunctiva initiated by Chlamydia trachomatis infection. This blinding disease affects over 40 million people worldwide yet the mechanisms underlying its pathogenesis remain poorly understood. We have investigated host microRNA (miR) expression in heal...

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Autores principales: Derrick, Tamsyn, Roberts, Chrissy h., Rajasekhar, Megha, Burr, Sarah E., Joof, Hassan, Makalo, Pateh, Bailey, Robin L., Mabey, David C. W., Burton, Matthew J., Holland, Martin J.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3597489/
https://www.ncbi.nlm.nih.gov/pubmed/23516655
http://dx.doi.org/10.1371/journal.pntd.0002117
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author Derrick, Tamsyn
Roberts, Chrissy h.
Rajasekhar, Megha
Burr, Sarah E.
Joof, Hassan
Makalo, Pateh
Bailey, Robin L.
Mabey, David C. W.
Burton, Matthew J.
Holland, Martin J.
author_facet Derrick, Tamsyn
Roberts, Chrissy h.
Rajasekhar, Megha
Burr, Sarah E.
Joof, Hassan
Makalo, Pateh
Bailey, Robin L.
Mabey, David C. W.
Burton, Matthew J.
Holland, Martin J.
author_sort Derrick, Tamsyn
collection PubMed
description PURPOSE: Trachoma is a fibrotic disease of the conjunctiva initiated by Chlamydia trachomatis infection. This blinding disease affects over 40 million people worldwide yet the mechanisms underlying its pathogenesis remain poorly understood. We have investigated host microRNA (miR) expression in health (N) and disease (conjunctival scarring with (TSI) and without (TS) inflammation) to determine if these epigenetic differences are associated with pathology. METHODS: We collected two independent samples of human conjunctival swab specimens from individuals living in The Gambia (n = 63 & 194). miR was extracted, and we investigated the expression of 754 miR in the first sample of 63 specimens (23 N, 17 TS, 23 TSI) using Taqman qPCR array human miRNA genecards. Network and pathway analysis was performed on this dataset. Seven miR that were significantly differentially expressed between different phenotypic groups were then selected for validation by qPCR in the second sample of 194 specimens (93 N, 74 TS, 22 TSI). RESULTS: Array screening revealed differential expression of 82 miR between N, TS and TSI phenotypes (fold change >3, p<0.05). Predicted mRNA targets of these miR were enriched in pathways involved in fibrosis and epithelial cell differentiation. Two miR were confirmed as being differentially expressed upon validation by qPCR. miR-147b is significantly up-regulated in TSI versus N (fold change = 2.3, p = 0.03) and miR-1285 is up-regulated in TSI versus TS (fold change = 4.6, p = 0.005), which was consistent with the results of the qPCR array. CONCLUSIONS: miR-147b and miR-1285 are up-regulated in inflammatory trachomatous scarring. Further investigation of the function of these miR will aid our understanding of the pathogenesis of trachoma.
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spelling pubmed-35974892013-03-20 Conjunctival MicroRNA Expression in Inflammatory Trachomatous Scarring Derrick, Tamsyn Roberts, Chrissy h. Rajasekhar, Megha Burr, Sarah E. Joof, Hassan Makalo, Pateh Bailey, Robin L. Mabey, David C. W. Burton, Matthew J. Holland, Martin J. PLoS Negl Trop Dis Research Article PURPOSE: Trachoma is a fibrotic disease of the conjunctiva initiated by Chlamydia trachomatis infection. This blinding disease affects over 40 million people worldwide yet the mechanisms underlying its pathogenesis remain poorly understood. We have investigated host microRNA (miR) expression in health (N) and disease (conjunctival scarring with (TSI) and without (TS) inflammation) to determine if these epigenetic differences are associated with pathology. METHODS: We collected two independent samples of human conjunctival swab specimens from individuals living in The Gambia (n = 63 & 194). miR was extracted, and we investigated the expression of 754 miR in the first sample of 63 specimens (23 N, 17 TS, 23 TSI) using Taqman qPCR array human miRNA genecards. Network and pathway analysis was performed on this dataset. Seven miR that were significantly differentially expressed between different phenotypic groups were then selected for validation by qPCR in the second sample of 194 specimens (93 N, 74 TS, 22 TSI). RESULTS: Array screening revealed differential expression of 82 miR between N, TS and TSI phenotypes (fold change >3, p<0.05). Predicted mRNA targets of these miR were enriched in pathways involved in fibrosis and epithelial cell differentiation. Two miR were confirmed as being differentially expressed upon validation by qPCR. miR-147b is significantly up-regulated in TSI versus N (fold change = 2.3, p = 0.03) and miR-1285 is up-regulated in TSI versus TS (fold change = 4.6, p = 0.005), which was consistent with the results of the qPCR array. CONCLUSIONS: miR-147b and miR-1285 are up-regulated in inflammatory trachomatous scarring. Further investigation of the function of these miR will aid our understanding of the pathogenesis of trachoma. Public Library of Science 2013-03-14 /pmc/articles/PMC3597489/ /pubmed/23516655 http://dx.doi.org/10.1371/journal.pntd.0002117 Text en © 2013 Derrick et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Derrick, Tamsyn
Roberts, Chrissy h.
Rajasekhar, Megha
Burr, Sarah E.
Joof, Hassan
Makalo, Pateh
Bailey, Robin L.
Mabey, David C. W.
Burton, Matthew J.
Holland, Martin J.
Conjunctival MicroRNA Expression in Inflammatory Trachomatous Scarring
title Conjunctival MicroRNA Expression in Inflammatory Trachomatous Scarring
title_full Conjunctival MicroRNA Expression in Inflammatory Trachomatous Scarring
title_fullStr Conjunctival MicroRNA Expression in Inflammatory Trachomatous Scarring
title_full_unstemmed Conjunctival MicroRNA Expression in Inflammatory Trachomatous Scarring
title_short Conjunctival MicroRNA Expression in Inflammatory Trachomatous Scarring
title_sort conjunctival microrna expression in inflammatory trachomatous scarring
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3597489/
https://www.ncbi.nlm.nih.gov/pubmed/23516655
http://dx.doi.org/10.1371/journal.pntd.0002117
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