A Real-Time PCR Assay for the Detection of Atypical Strains of Chlamydiaceae from Pigeons

Recent evidence of the occurrence of atypical Chlamydiaceae strains in pigeons, different from the established Chlamydiaceae, requires the development of a specific and rapid detection tool to investigate their prevalence and significance. Here is described a new real-time PCR assay that allows spec...

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Detalles Bibliográficos
Autores principales: Zocevic, Aleksandar, Vorimore, Fabien, Vicari, Nadia, Gasparini, Julien, Jacquin, Lisa, Sachse, Konrad, Magnino, Simone, Laroucau, Karine
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2013
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3597529/
https://www.ncbi.nlm.nih.gov/pubmed/23516548
http://dx.doi.org/10.1371/journal.pone.0058741
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author Zocevic, Aleksandar
Vorimore, Fabien
Vicari, Nadia
Gasparini, Julien
Jacquin, Lisa
Sachse, Konrad
Magnino, Simone
Laroucau, Karine
author_facet Zocevic, Aleksandar
Vorimore, Fabien
Vicari, Nadia
Gasparini, Julien
Jacquin, Lisa
Sachse, Konrad
Magnino, Simone
Laroucau, Karine
author_sort Zocevic, Aleksandar
collection PubMed
description Recent evidence of the occurrence of atypical Chlamydiaceae strains in pigeons, different from the established Chlamydiaceae, requires the development of a specific and rapid detection tool to investigate their prevalence and significance. Here is described a new real-time PCR assay that allows specific detection of atypical Chlamydiaceae from pigeons. The assay has been used to assess the dissemination of these strains in field samples collected from Parisian pigeon populations in 2009. The results suggest a limited dissemination compared to the usually higher prevalence of Chlamydia psittaci that is the main species associated with avian chlamydiosis.
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spelling pubmed-35975292013-03-20 A Real-Time PCR Assay for the Detection of Atypical Strains of Chlamydiaceae from Pigeons Zocevic, Aleksandar Vorimore, Fabien Vicari, Nadia Gasparini, Julien Jacquin, Lisa Sachse, Konrad Magnino, Simone Laroucau, Karine PLoS One Research Article Recent evidence of the occurrence of atypical Chlamydiaceae strains in pigeons, different from the established Chlamydiaceae, requires the development of a specific and rapid detection tool to investigate their prevalence and significance. Here is described a new real-time PCR assay that allows specific detection of atypical Chlamydiaceae from pigeons. The assay has been used to assess the dissemination of these strains in field samples collected from Parisian pigeon populations in 2009. The results suggest a limited dissemination compared to the usually higher prevalence of Chlamydia psittaci that is the main species associated with avian chlamydiosis. Public Library of Science 2013-03-14 /pmc/articles/PMC3597529/ /pubmed/23516548 http://dx.doi.org/10.1371/journal.pone.0058741 Text en © 2013 Zocevic et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Zocevic, Aleksandar
Vorimore, Fabien
Vicari, Nadia
Gasparini, Julien
Jacquin, Lisa
Sachse, Konrad
Magnino, Simone
Laroucau, Karine
A Real-Time PCR Assay for the Detection of Atypical Strains of Chlamydiaceae from Pigeons
title A Real-Time PCR Assay for the Detection of Atypical Strains of Chlamydiaceae from Pigeons
title_full A Real-Time PCR Assay for the Detection of Atypical Strains of Chlamydiaceae from Pigeons
title_fullStr A Real-Time PCR Assay for the Detection of Atypical Strains of Chlamydiaceae from Pigeons
title_full_unstemmed A Real-Time PCR Assay for the Detection of Atypical Strains of Chlamydiaceae from Pigeons
title_short A Real-Time PCR Assay for the Detection of Atypical Strains of Chlamydiaceae from Pigeons
title_sort real-time pcr assay for the detection of atypical strains of chlamydiaceae from pigeons
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3597529/
https://www.ncbi.nlm.nih.gov/pubmed/23516548
http://dx.doi.org/10.1371/journal.pone.0058741
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