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Dual function of MIPS1 as a metabolic enzyme and transcriptional regulator

Because regulation of its activity is instrumental either to support cell proliferation and growth or to promote cell death, the universal myo-inositol phosphate synthase (MIPS), responsible for myo-inositol biosynthesis, is a critical enzyme of primary metabolism. Surprisingly, we found this enzyme...

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Autores principales: Latrasse, David, Jégu, Teddy, Meng, Pin-Hong, Mazubert, Christelle, Hudik, Elodie, Delarue, Marianne, Charon, Céline, Crespi, Martin, Hirt, Heribert, Raynaud, Cécile, Bergounioux, Catherine, Benhamed, Moussa
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3597657/
https://www.ncbi.nlm.nih.gov/pubmed/23341037
http://dx.doi.org/10.1093/nar/gks1458
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author Latrasse, David
Jégu, Teddy
Meng, Pin-Hong
Mazubert, Christelle
Hudik, Elodie
Delarue, Marianne
Charon, Céline
Crespi, Martin
Hirt, Heribert
Raynaud, Cécile
Bergounioux, Catherine
Benhamed, Moussa
author_facet Latrasse, David
Jégu, Teddy
Meng, Pin-Hong
Mazubert, Christelle
Hudik, Elodie
Delarue, Marianne
Charon, Céline
Crespi, Martin
Hirt, Heribert
Raynaud, Cécile
Bergounioux, Catherine
Benhamed, Moussa
author_sort Latrasse, David
collection PubMed
description Because regulation of its activity is instrumental either to support cell proliferation and growth or to promote cell death, the universal myo-inositol phosphate synthase (MIPS), responsible for myo-inositol biosynthesis, is a critical enzyme of primary metabolism. Surprisingly, we found this enzyme to be imported in the nucleus and to interact with the histone methyltransferases ATXR5 and ATXR6, raising the question of whether MIPS1 has a function in transcriptional regulation. Here, we demonstrate that MIPS1 binds directly to its promoter to stimulate its own expression by locally inhibiting the spreading of ATXR5/6-dependent heterochromatin marks coming from a transposable element. Furthermore, on activation of pathogen response, MIPS1 expression is reduced epigenetically, providing evidence for a complex regulatory mechanism acting at the transcriptional level. Thus, in plants, MIPS1 appears to have evolved as a protein that connects cellular metabolism, pathogen response and chromatin remodeling.
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spelling pubmed-35976572013-03-15 Dual function of MIPS1 as a metabolic enzyme and transcriptional regulator Latrasse, David Jégu, Teddy Meng, Pin-Hong Mazubert, Christelle Hudik, Elodie Delarue, Marianne Charon, Céline Crespi, Martin Hirt, Heribert Raynaud, Cécile Bergounioux, Catherine Benhamed, Moussa Nucleic Acids Res Gene Regulation, Chromatin and Epigenetics Because regulation of its activity is instrumental either to support cell proliferation and growth or to promote cell death, the universal myo-inositol phosphate synthase (MIPS), responsible for myo-inositol biosynthesis, is a critical enzyme of primary metabolism. Surprisingly, we found this enzyme to be imported in the nucleus and to interact with the histone methyltransferases ATXR5 and ATXR6, raising the question of whether MIPS1 has a function in transcriptional regulation. Here, we demonstrate that MIPS1 binds directly to its promoter to stimulate its own expression by locally inhibiting the spreading of ATXR5/6-dependent heterochromatin marks coming from a transposable element. Furthermore, on activation of pathogen response, MIPS1 expression is reduced epigenetically, providing evidence for a complex regulatory mechanism acting at the transcriptional level. Thus, in plants, MIPS1 appears to have evolved as a protein that connects cellular metabolism, pathogen response and chromatin remodeling. Oxford University Press 2013-03 2013-01-21 /pmc/articles/PMC3597657/ /pubmed/23341037 http://dx.doi.org/10.1093/nar/gks1458 Text en © The Author(s) 2013. Published by Oxford University Press. http://creativecommons.org/licenses/by-nc/3.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Gene Regulation, Chromatin and Epigenetics
Latrasse, David
Jégu, Teddy
Meng, Pin-Hong
Mazubert, Christelle
Hudik, Elodie
Delarue, Marianne
Charon, Céline
Crespi, Martin
Hirt, Heribert
Raynaud, Cécile
Bergounioux, Catherine
Benhamed, Moussa
Dual function of MIPS1 as a metabolic enzyme and transcriptional regulator
title Dual function of MIPS1 as a metabolic enzyme and transcriptional regulator
title_full Dual function of MIPS1 as a metabolic enzyme and transcriptional regulator
title_fullStr Dual function of MIPS1 as a metabolic enzyme and transcriptional regulator
title_full_unstemmed Dual function of MIPS1 as a metabolic enzyme and transcriptional regulator
title_short Dual function of MIPS1 as a metabolic enzyme and transcriptional regulator
title_sort dual function of mips1 as a metabolic enzyme and transcriptional regulator
topic Gene Regulation, Chromatin and Epigenetics
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3597657/
https://www.ncbi.nlm.nih.gov/pubmed/23341037
http://dx.doi.org/10.1093/nar/gks1458
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