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Molecular paleontology: a biochemical model of the ancestral ribosome
Ancient components of the ribosome, inferred from a consensus of previous work, were constructed in silico, in vitro and in vivo. The resulting model of the ancestral ribosome presented here incorporates ∼20% of the extant 23S rRNA and fragments of five ribosomal proteins. We test hypotheses that an...
Autores principales: | , , , , , , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Oxford University Press
2013
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3597689/ https://www.ncbi.nlm.nih.gov/pubmed/23355613 http://dx.doi.org/10.1093/nar/gkt023 |
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author | Hsiao, Chiaolong Lenz, Timothy K. Peters, Jessica K. Fang, Po-Yu Schneider, Dana M. Anderson, Eric J. Preeprem, Thanawadee Bowman, Jessica C. O'Neill, Eric B. Lie, Lively Athavale, Shreyas S. Gossett, J. Jared Trippe, Catherine Murray, Jason Petrov, Anton S. Wartell, Roger M. Harvey, Stephen C. Hud, Nicholas V. Dean Williams, Loren |
author_facet | Hsiao, Chiaolong Lenz, Timothy K. Peters, Jessica K. Fang, Po-Yu Schneider, Dana M. Anderson, Eric J. Preeprem, Thanawadee Bowman, Jessica C. O'Neill, Eric B. Lie, Lively Athavale, Shreyas S. Gossett, J. Jared Trippe, Catherine Murray, Jason Petrov, Anton S. Wartell, Roger M. Harvey, Stephen C. Hud, Nicholas V. Dean Williams, Loren |
author_sort | Hsiao, Chiaolong |
collection | PubMed |
description | Ancient components of the ribosome, inferred from a consensus of previous work, were constructed in silico, in vitro and in vivo. The resulting model of the ancestral ribosome presented here incorporates ∼20% of the extant 23S rRNA and fragments of five ribosomal proteins. We test hypotheses that ancestral rRNA can: (i) assume canonical 23S rRNA-like secondary structure, (ii) assume canonical tertiary structure and (iii) form native complexes with ribosomal protein fragments. Footprinting experiments support formation of predicted secondary and tertiary structure. Gel shift, spectroscopic and yeast three-hybrid assays show specific interactions between ancestral rRNA and ribosomal protein fragments, independent of other, more recent, components of the ribosome. This robustness suggests that the catalytic core of the ribosome is an ancient construct that has survived billions of years of evolution without major changes in structure. Collectively, the data here support a model in which ancestors of the large and small subunits originated and evolved independently of each other, with autonomous functionalities. |
format | Online Article Text |
id | pubmed-3597689 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2013 |
publisher | Oxford University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-35976892013-03-15 Molecular paleontology: a biochemical model of the ancestral ribosome Hsiao, Chiaolong Lenz, Timothy K. Peters, Jessica K. Fang, Po-Yu Schneider, Dana M. Anderson, Eric J. Preeprem, Thanawadee Bowman, Jessica C. O'Neill, Eric B. Lie, Lively Athavale, Shreyas S. Gossett, J. Jared Trippe, Catherine Murray, Jason Petrov, Anton S. Wartell, Roger M. Harvey, Stephen C. Hud, Nicholas V. Dean Williams, Loren Nucleic Acids Res RNA Ancient components of the ribosome, inferred from a consensus of previous work, were constructed in silico, in vitro and in vivo. The resulting model of the ancestral ribosome presented here incorporates ∼20% of the extant 23S rRNA and fragments of five ribosomal proteins. We test hypotheses that ancestral rRNA can: (i) assume canonical 23S rRNA-like secondary structure, (ii) assume canonical tertiary structure and (iii) form native complexes with ribosomal protein fragments. Footprinting experiments support formation of predicted secondary and tertiary structure. Gel shift, spectroscopic and yeast three-hybrid assays show specific interactions between ancestral rRNA and ribosomal protein fragments, independent of other, more recent, components of the ribosome. This robustness suggests that the catalytic core of the ribosome is an ancient construct that has survived billions of years of evolution without major changes in structure. Collectively, the data here support a model in which ancestors of the large and small subunits originated and evolved independently of each other, with autonomous functionalities. Oxford University Press 2013-03 2013-01-25 /pmc/articles/PMC3597689/ /pubmed/23355613 http://dx.doi.org/10.1093/nar/gkt023 Text en © The Author(s) 2013. Published by Oxford University Press. http://creativecommons.org/licenses/by-nc/3.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | RNA Hsiao, Chiaolong Lenz, Timothy K. Peters, Jessica K. Fang, Po-Yu Schneider, Dana M. Anderson, Eric J. Preeprem, Thanawadee Bowman, Jessica C. O'Neill, Eric B. Lie, Lively Athavale, Shreyas S. Gossett, J. Jared Trippe, Catherine Murray, Jason Petrov, Anton S. Wartell, Roger M. Harvey, Stephen C. Hud, Nicholas V. Dean Williams, Loren Molecular paleontology: a biochemical model of the ancestral ribosome |
title | Molecular paleontology: a biochemical model of the ancestral ribosome |
title_full | Molecular paleontology: a biochemical model of the ancestral ribosome |
title_fullStr | Molecular paleontology: a biochemical model of the ancestral ribosome |
title_full_unstemmed | Molecular paleontology: a biochemical model of the ancestral ribosome |
title_short | Molecular paleontology: a biochemical model of the ancestral ribosome |
title_sort | molecular paleontology: a biochemical model of the ancestral ribosome |
topic | RNA |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3597689/ https://www.ncbi.nlm.nih.gov/pubmed/23355613 http://dx.doi.org/10.1093/nar/gkt023 |
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