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Detection of ESBL among ampc producing enterobacteriaceae using inhibitor-based method
INTRODUCTION: The occurrence of multiple β-lactamases among bacteria only limits the therapeutic options but also poses a challenge. A study using boronic acid (BA), an AmpC enzyme inhibitor, was designed to detect the combined expression of AmpC β-lactamases and extended-spectrum β-lactamases (ESBL...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
The African Field Epidemiology Network
2013
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3597859/ https://www.ncbi.nlm.nih.gov/pubmed/23504148 http://dx.doi.org/10.11604/pamj.2013.14.28.1347 |
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author | Bakthavatchalu, Sasirekha Shakthivel, Uma Mishra, Tannu |
author_facet | Bakthavatchalu, Sasirekha Shakthivel, Uma Mishra, Tannu |
author_sort | Bakthavatchalu, Sasirekha |
collection | PubMed |
description | INTRODUCTION: The occurrence of multiple β-lactamases among bacteria only limits the therapeutic options but also poses a challenge. A study using boronic acid (BA), an AmpC enzyme inhibitor, was designed to detect the combined expression of AmpC β-lactamases and extended-spectrum β-lactamases (ESBLs) in bacterial isolates further different phenotypic methods are compared to detect ESBL and AmpC. METHODS: A total of 259 clinical isolates of Enterobacteriaceae were isolated and screened for ESBL production by (i) CLSI double-disk diffusion method (ii) cefepime- clavulanic acid method (iii) boronic disk potentiation method. AmpC production was detected using cefoxitin alone and in combination with boronic acid and confirmation was done by three dimensional disk methods. Isolates were also subjected to detailed antibiotic susceptibility test. RESULTS: Among 259 isolates, 20.46% were coproducers of ESBL and AmpC, 26.45% were ESBL and 5.40% were AmpC. All of the 53 AmpC and ESBL coproducers were accurately detected by boronic acid disk potentiation method. CONCLUSION: The BA disk test using Clinical and Laboratory Standards Institute methodology is simple and very efficient method that accurately detects the isolates that harbor both AmpCs and ESBLs. |
format | Online Article Text |
id | pubmed-3597859 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2013 |
publisher | The African Field Epidemiology Network |
record_format | MEDLINE/PubMed |
spelling | pubmed-35978592013-03-15 Detection of ESBL among ampc producing enterobacteriaceae using inhibitor-based method Bakthavatchalu, Sasirekha Shakthivel, Uma Mishra, Tannu Pan Afr Med J Research INTRODUCTION: The occurrence of multiple β-lactamases among bacteria only limits the therapeutic options but also poses a challenge. A study using boronic acid (BA), an AmpC enzyme inhibitor, was designed to detect the combined expression of AmpC β-lactamases and extended-spectrum β-lactamases (ESBLs) in bacterial isolates further different phenotypic methods are compared to detect ESBL and AmpC. METHODS: A total of 259 clinical isolates of Enterobacteriaceae were isolated and screened for ESBL production by (i) CLSI double-disk diffusion method (ii) cefepime- clavulanic acid method (iii) boronic disk potentiation method. AmpC production was detected using cefoxitin alone and in combination with boronic acid and confirmation was done by three dimensional disk methods. Isolates were also subjected to detailed antibiotic susceptibility test. RESULTS: Among 259 isolates, 20.46% were coproducers of ESBL and AmpC, 26.45% were ESBL and 5.40% were AmpC. All of the 53 AmpC and ESBL coproducers were accurately detected by boronic acid disk potentiation method. CONCLUSION: The BA disk test using Clinical and Laboratory Standards Institute methodology is simple and very efficient method that accurately detects the isolates that harbor both AmpCs and ESBLs. The African Field Epidemiology Network 2013-01-20 /pmc/articles/PMC3597859/ /pubmed/23504148 http://dx.doi.org/10.11604/pamj.2013.14.28.1347 Text en © Sasirekha Bakthavatchalu et al. http://creativecommons.org/licenses/by/2.0/ The Pan African Medical Journal - ISSN 1937-8688. This is an Open Access article distributed under the terms of the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Bakthavatchalu, Sasirekha Shakthivel, Uma Mishra, Tannu Detection of ESBL among ampc producing enterobacteriaceae using inhibitor-based method |
title | Detection of ESBL among ampc producing enterobacteriaceae using inhibitor-based method |
title_full | Detection of ESBL among ampc producing enterobacteriaceae using inhibitor-based method |
title_fullStr | Detection of ESBL among ampc producing enterobacteriaceae using inhibitor-based method |
title_full_unstemmed | Detection of ESBL among ampc producing enterobacteriaceae using inhibitor-based method |
title_short | Detection of ESBL among ampc producing enterobacteriaceae using inhibitor-based method |
title_sort | detection of esbl among ampc producing enterobacteriaceae using inhibitor-based method |
topic | Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3597859/ https://www.ncbi.nlm.nih.gov/pubmed/23504148 http://dx.doi.org/10.11604/pamj.2013.14.28.1347 |
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