Cargando…

Antimicrobial susceptibility testing in 90 min by bacterial cell count monitoring

The rise in antimicrobial resistance has become a serious global health problem. Restrictive use of antibiotics seems the only option to temper this accession since research in new antibiotics has halted. Antimicrobial stewardship programmes rely on quick access to susceptibility data. This study ev...

Descripción completa

Detalles Bibliográficos
Autores principales: Broeren, M A C, Maas, Y, Retera, E, Arents, N L A
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Blackwell Publishing Ltd 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3597975/
https://www.ncbi.nlm.nih.gov/pubmed/22390723
http://dx.doi.org/10.1111/j.1469-0691.2012.03800.x
_version_ 1782262706626101248
author Broeren, M A C
Maas, Y
Retera, E
Arents, N L A
author_facet Broeren, M A C
Maas, Y
Retera, E
Arents, N L A
author_sort Broeren, M A C
collection PubMed
description The rise in antimicrobial resistance has become a serious global health problem. Restrictive use of antibiotics seems the only option to temper this accession since research in new antibiotics has halted. Antimicrobial stewardship programmes rely on quick access to susceptibility data. This study evaluated the concept of bacterial cell count monitoring as a fast method to determine susceptibility. Escherichia coli, Pseudomonas aeruginosa and Staphylococcus aureus strains were tested for amoxicillin/piperacillin and gentamicin by three conventional methods (VITEK2®, Etest® and broth-macrodilution). Bacterial cell count monitoring reliably predicted susceptibility after 90 min for Escherichia coli and after 120 min for Pseudomonas aeruginosa and Staphylococcus aureus without any minor, major or very major discrepancies. Time-to-result was reduced by 74%, 83% and 76%, respectively. Bacterial cell count monitoring shows great potential for rapid susceptibility testing.
format Online
Article
Text
id pubmed-3597975
institution National Center for Biotechnology Information
language English
publishDate 2013
publisher Blackwell Publishing Ltd
record_format MEDLINE/PubMed
spelling pubmed-35979752013-03-19 Antimicrobial susceptibility testing in 90 min by bacterial cell count monitoring Broeren, M A C Maas, Y Retera, E Arents, N L A Clin Microbiol Infect Bacteriology The rise in antimicrobial resistance has become a serious global health problem. Restrictive use of antibiotics seems the only option to temper this accession since research in new antibiotics has halted. Antimicrobial stewardship programmes rely on quick access to susceptibility data. This study evaluated the concept of bacterial cell count monitoring as a fast method to determine susceptibility. Escherichia coli, Pseudomonas aeruginosa and Staphylococcus aureus strains were tested for amoxicillin/piperacillin and gentamicin by three conventional methods (VITEK2®, Etest® and broth-macrodilution). Bacterial cell count monitoring reliably predicted susceptibility after 90 min for Escherichia coli and after 120 min for Pseudomonas aeruginosa and Staphylococcus aureus without any minor, major or very major discrepancies. Time-to-result was reduced by 74%, 83% and 76%, respectively. Bacterial cell count monitoring shows great potential for rapid susceptibility testing. Blackwell Publishing Ltd 2013-03 2012-02-09 /pmc/articles/PMC3597975/ /pubmed/22390723 http://dx.doi.org/10.1111/j.1469-0691.2012.03800.x Text en © 2012 The Authors. Clinical Microbiology and Infection © 2012 European Society of Clinical Microbiology and Infectious Diseases http://creativecommons.org/licenses/by/2.5/ Re-use of this article is permitted in accordance with the Creative Commons Deed, Attribution 2.5, which does not permit commercial exploitation.
spellingShingle Bacteriology
Broeren, M A C
Maas, Y
Retera, E
Arents, N L A
Antimicrobial susceptibility testing in 90 min by bacterial cell count monitoring
title Antimicrobial susceptibility testing in 90 min by bacterial cell count monitoring
title_full Antimicrobial susceptibility testing in 90 min by bacterial cell count monitoring
title_fullStr Antimicrobial susceptibility testing in 90 min by bacterial cell count monitoring
title_full_unstemmed Antimicrobial susceptibility testing in 90 min by bacterial cell count monitoring
title_short Antimicrobial susceptibility testing in 90 min by bacterial cell count monitoring
title_sort antimicrobial susceptibility testing in 90 min by bacterial cell count monitoring
topic Bacteriology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3597975/
https://www.ncbi.nlm.nih.gov/pubmed/22390723
http://dx.doi.org/10.1111/j.1469-0691.2012.03800.x
work_keys_str_mv AT broerenmac antimicrobialsusceptibilitytestingin90minbybacterialcellcountmonitoring
AT maasy antimicrobialsusceptibilitytestingin90minbybacterialcellcountmonitoring
AT reterae antimicrobialsusceptibilitytestingin90minbybacterialcellcountmonitoring
AT arentsnla antimicrobialsusceptibilitytestingin90minbybacterialcellcountmonitoring