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Phenotype profiling of Rhizobium leguminosarum bv. trifolii clover nodule isolates reveal their both versatile and specialized metabolic capabilities

Rhizobium leguminosarum bv. trifolii (Rlt) are soil bacteria inducing nodules on clover, where they fix nitrogen. Genome organization analyses of 22 Rlt clover nodule isolates showed that they contained 3–6 plasmids and majority of them possessed large (>1 Mb), chromid-like replicon with exceptio...

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Autores principales: Mazur, Andrzej, Stasiak, Grażyna, Wielbo, Jerzy, Koper, Piotr, Kubik-Komar, Agnieszka, Skorupska, Anna
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer-Verlag 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3597991/
https://www.ncbi.nlm.nih.gov/pubmed/23417392
http://dx.doi.org/10.1007/s00203-013-0874-x
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author Mazur, Andrzej
Stasiak, Grażyna
Wielbo, Jerzy
Koper, Piotr
Kubik-Komar, Agnieszka
Skorupska, Anna
author_facet Mazur, Andrzej
Stasiak, Grażyna
Wielbo, Jerzy
Koper, Piotr
Kubik-Komar, Agnieszka
Skorupska, Anna
author_sort Mazur, Andrzej
collection PubMed
description Rhizobium leguminosarum bv. trifolii (Rlt) are soil bacteria inducing nodules on clover, where they fix nitrogen. Genome organization analyses of 22 Rlt clover nodule isolates showed that they contained 3–6 plasmids and majority of them possessed large (>1 Mb), chromid-like replicon with exception of four Rlt strains. The Biolog phenotypic profiling comprising utilization of C, N, P, and S sources and tolerance to osmolytes and pH revealed metabolic versatility of the Rlt strains. Statistical analyses of our results showed a clear bias toward specific metabolic preferences, tolerance to unfavorable osmotic conditions, and increased nodulation activity of the strains having smaller amount of extrachromosomal DNA. The K5.4 and K4.15 lacking a large megaplasmid possessed substantially diverse metabolism and belonged to effective clover inoculants. In conclusion, besides overall metabolic versatility, some metabolic specialization may enable rhizobia to persist in variable environments and to compete successfully with other bacteria. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1007/s00203-013-0874-x) contains supplementary material, which is available to authorized users.
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spelling pubmed-35979912013-03-19 Phenotype profiling of Rhizobium leguminosarum bv. trifolii clover nodule isolates reveal their both versatile and specialized metabolic capabilities Mazur, Andrzej Stasiak, Grażyna Wielbo, Jerzy Koper, Piotr Kubik-Komar, Agnieszka Skorupska, Anna Arch Microbiol Original Paper Rhizobium leguminosarum bv. trifolii (Rlt) are soil bacteria inducing nodules on clover, where they fix nitrogen. Genome organization analyses of 22 Rlt clover nodule isolates showed that they contained 3–6 plasmids and majority of them possessed large (>1 Mb), chromid-like replicon with exception of four Rlt strains. The Biolog phenotypic profiling comprising utilization of C, N, P, and S sources and tolerance to osmolytes and pH revealed metabolic versatility of the Rlt strains. Statistical analyses of our results showed a clear bias toward specific metabolic preferences, tolerance to unfavorable osmotic conditions, and increased nodulation activity of the strains having smaller amount of extrachromosomal DNA. The K5.4 and K4.15 lacking a large megaplasmid possessed substantially diverse metabolism and belonged to effective clover inoculants. In conclusion, besides overall metabolic versatility, some metabolic specialization may enable rhizobia to persist in variable environments and to compete successfully with other bacteria. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1007/s00203-013-0874-x) contains supplementary material, which is available to authorized users. Springer-Verlag 2013-02-16 2013 /pmc/articles/PMC3597991/ /pubmed/23417392 http://dx.doi.org/10.1007/s00203-013-0874-x Text en © The Author(s) 2013 https://creativecommons.org/licenses/by/2.0/ Open AccessThis article is distributed under the terms of the Creative Commons Attribution License which permits any use, distribution, and reproduction in any medium, provided the original author(s) and the source are credited.
spellingShingle Original Paper
Mazur, Andrzej
Stasiak, Grażyna
Wielbo, Jerzy
Koper, Piotr
Kubik-Komar, Agnieszka
Skorupska, Anna
Phenotype profiling of Rhizobium leguminosarum bv. trifolii clover nodule isolates reveal their both versatile and specialized metabolic capabilities
title Phenotype profiling of Rhizobium leguminosarum bv. trifolii clover nodule isolates reveal their both versatile and specialized metabolic capabilities
title_full Phenotype profiling of Rhizobium leguminosarum bv. trifolii clover nodule isolates reveal their both versatile and specialized metabolic capabilities
title_fullStr Phenotype profiling of Rhizobium leguminosarum bv. trifolii clover nodule isolates reveal their both versatile and specialized metabolic capabilities
title_full_unstemmed Phenotype profiling of Rhizobium leguminosarum bv. trifolii clover nodule isolates reveal their both versatile and specialized metabolic capabilities
title_short Phenotype profiling of Rhizobium leguminosarum bv. trifolii clover nodule isolates reveal their both versatile and specialized metabolic capabilities
title_sort phenotype profiling of rhizobium leguminosarum bv. trifolii clover nodule isolates reveal their both versatile and specialized metabolic capabilities
topic Original Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3597991/
https://www.ncbi.nlm.nih.gov/pubmed/23417392
http://dx.doi.org/10.1007/s00203-013-0874-x
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