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Thyroid hormone increases fibroblast growth factor receptor expression and disrupts cell mechanics in the developing organ of corti
BACKGROUND: Thyroid hormones regulate growth and development. However, the molecular mechanisms by which thyroid hormone regulates cell structural development are not fully understood. The mammalian cochlea is an intriguing system to examine these mechanisms, as cellular structure plays a key role i...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2013
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3598248/ https://www.ncbi.nlm.nih.gov/pubmed/23394545 http://dx.doi.org/10.1186/1471-213X-13-6 |
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author | Szarama, Katherine B Gavara, Núria Petralia, Ronald S Chadwick, Richard S Kelley, Matthew W |
author_facet | Szarama, Katherine B Gavara, Núria Petralia, Ronald S Chadwick, Richard S Kelley, Matthew W |
author_sort | Szarama, Katherine B |
collection | PubMed |
description | BACKGROUND: Thyroid hormones regulate growth and development. However, the molecular mechanisms by which thyroid hormone regulates cell structural development are not fully understood. The mammalian cochlea is an intriguing system to examine these mechanisms, as cellular structure plays a key role in tissue development, and thyroid hormone is required for the maturation of the cochlea in the first postnatal week. RESULTS: In hypothyroid conditions, we found disruptions in sensory outer hair cell morphology and fewer microtubules in non-sensory supporting pillar cells. To test the functional consequences of these cytoskeletal defects on cell mechanics, we combined atomic force microscopy with live cell imaging. Hypothyroidism stiffened outer hair cells and supporting pillar cells, but pillar cells ultimately showed reduced cell stiffness, in part from a lack of microtubules. Analyses of changes in transcription and protein phosphorylation suggest that hypothyroidism prolonged expression of fibroblast growth factor receptors, and decreased phosphorylated Cofilin. CONCLUSIONS: These findings demonstrate that thyroid hormones may be involved in coordinating the processes that regulate cytoskeletal dynamics and suggest that manipulating thyroid hormone sensitivity might provide insight into the relationship between cytoskeletal formation and developing cell mechanical properties. |
format | Online Article Text |
id | pubmed-3598248 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2013 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-35982482013-03-16 Thyroid hormone increases fibroblast growth factor receptor expression and disrupts cell mechanics in the developing organ of corti Szarama, Katherine B Gavara, Núria Petralia, Ronald S Chadwick, Richard S Kelley, Matthew W BMC Dev Biol Research Article BACKGROUND: Thyroid hormones regulate growth and development. However, the molecular mechanisms by which thyroid hormone regulates cell structural development are not fully understood. The mammalian cochlea is an intriguing system to examine these mechanisms, as cellular structure plays a key role in tissue development, and thyroid hormone is required for the maturation of the cochlea in the first postnatal week. RESULTS: In hypothyroid conditions, we found disruptions in sensory outer hair cell morphology and fewer microtubules in non-sensory supporting pillar cells. To test the functional consequences of these cytoskeletal defects on cell mechanics, we combined atomic force microscopy with live cell imaging. Hypothyroidism stiffened outer hair cells and supporting pillar cells, but pillar cells ultimately showed reduced cell stiffness, in part from a lack of microtubules. Analyses of changes in transcription and protein phosphorylation suggest that hypothyroidism prolonged expression of fibroblast growth factor receptors, and decreased phosphorylated Cofilin. CONCLUSIONS: These findings demonstrate that thyroid hormones may be involved in coordinating the processes that regulate cytoskeletal dynamics and suggest that manipulating thyroid hormone sensitivity might provide insight into the relationship between cytoskeletal formation and developing cell mechanical properties. BioMed Central 2013-02-09 /pmc/articles/PMC3598248/ /pubmed/23394545 http://dx.doi.org/10.1186/1471-213X-13-6 Text en Copyright ©2013 Szarama et al; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Szarama, Katherine B Gavara, Núria Petralia, Ronald S Chadwick, Richard S Kelley, Matthew W Thyroid hormone increases fibroblast growth factor receptor expression and disrupts cell mechanics in the developing organ of corti |
title | Thyroid hormone increases fibroblast growth factor receptor expression and disrupts cell mechanics in the developing organ of corti |
title_full | Thyroid hormone increases fibroblast growth factor receptor expression and disrupts cell mechanics in the developing organ of corti |
title_fullStr | Thyroid hormone increases fibroblast growth factor receptor expression and disrupts cell mechanics in the developing organ of corti |
title_full_unstemmed | Thyroid hormone increases fibroblast growth factor receptor expression and disrupts cell mechanics in the developing organ of corti |
title_short | Thyroid hormone increases fibroblast growth factor receptor expression and disrupts cell mechanics in the developing organ of corti |
title_sort | thyroid hormone increases fibroblast growth factor receptor expression and disrupts cell mechanics in the developing organ of corti |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3598248/ https://www.ncbi.nlm.nih.gov/pubmed/23394545 http://dx.doi.org/10.1186/1471-213X-13-6 |
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