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Validation of reference genes for quantitative real-time PCR studies in the dentate gyrus after experimental febrile seizures

BACKGROUND: Quantitative real-time PCR (qPCR) is a commonly used technique to quantify gene expression levels. Validated normalization is essential to obtain reliable qPCR data. In that context, normalizing to multiple reference genes has become the most popular method. However, expression of refere...

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Detalles Bibliográficos
Autores principales: Swijsen, Ann, Nelissen, Katherine, Janssen, Daniel, Rigo, Jean-Michel, Hoogland, Govert
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3598510/
https://www.ncbi.nlm.nih.gov/pubmed/23237195
http://dx.doi.org/10.1186/1756-0500-5-685