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A new method to customize protein expression vectors for fast, efficient and background free parallel cloning
BACKGROUND: Expression and purification of correctly folded proteins typically require screening of different parameters such as protein variants, solubility enhancing tags or expression hosts. Parallel vector series that cover all variations are available, but not without compromise. We have establ...
Autores principales: | Scholz, Judith, Besir, Hüseyin, Strasser, Claudia, Suppmann, Sabine |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2013
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3598636/ https://www.ncbi.nlm.nih.gov/pubmed/23410102 http://dx.doi.org/10.1186/1472-6750-13-12 |
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