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Digital Gene Expression Tag Profiling Analysis of the Gene Expression Patterns Regulating the Early Stage of Mouse Spermatogenesis

Detailed characterization of the gene expression patterns in spermatogonia and primary spermatocytes is critical to understand the processes which occur prior to meiosis during normal spermatogenesis. The genome-wide expression profiles of mouse type B spermatogonia and primary spermatocytes were in...

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Autores principales: Zhang, Xiujun, Hao, Lili, Meng, Lijun, Liu, Meiling, Zhao, Lina, Hu, Fen, Ding, Cunbao, Wang, Yang, He, Baoling, Pan, Yuxin, Fang, Wei, Chen, Jing, Hu, Songnian, Jia, Mengchun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3598852/
https://www.ncbi.nlm.nih.gov/pubmed/23554914
http://dx.doi.org/10.1371/journal.pone.0058680
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author Zhang, Xiujun
Hao, Lili
Meng, Lijun
Liu, Meiling
Zhao, Lina
Hu, Fen
Ding, Cunbao
Wang, Yang
He, Baoling
Pan, Yuxin
Fang, Wei
Chen, Jing
Hu, Songnian
Jia, Mengchun
author_facet Zhang, Xiujun
Hao, Lili
Meng, Lijun
Liu, Meiling
Zhao, Lina
Hu, Fen
Ding, Cunbao
Wang, Yang
He, Baoling
Pan, Yuxin
Fang, Wei
Chen, Jing
Hu, Songnian
Jia, Mengchun
author_sort Zhang, Xiujun
collection PubMed
description Detailed characterization of the gene expression patterns in spermatogonia and primary spermatocytes is critical to understand the processes which occur prior to meiosis during normal spermatogenesis. The genome-wide expression profiles of mouse type B spermatogonia and primary spermatocytes were investigated using the Solexa/Illumina digital gene expression (DGE) system, a tag based high-throughput transcriptome sequencing method, and the developmental processes which occur during early spermatogenesis were systematically analyzed. Gene expression patterns vary significantly between mouse type B spermatogonia and primary spermatocytes. The functional analysis revealed that genes related to junction assembly, regulation of the actin cytoskeleton and pluripotency were most significantly differently expressed. Pathway analysis indicated that the Wnt non-canonical signaling pathway played a central role and interacted with the actin filament organization pathway during the development of spermatogonia. This study provides a foundation for further analysis of the gene expression patterns and signaling pathways which regulate the molecular mechanisms of early spermatogenesis.
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spelling pubmed-35988522013-04-02 Digital Gene Expression Tag Profiling Analysis of the Gene Expression Patterns Regulating the Early Stage of Mouse Spermatogenesis Zhang, Xiujun Hao, Lili Meng, Lijun Liu, Meiling Zhao, Lina Hu, Fen Ding, Cunbao Wang, Yang He, Baoling Pan, Yuxin Fang, Wei Chen, Jing Hu, Songnian Jia, Mengchun PLoS One Research Article Detailed characterization of the gene expression patterns in spermatogonia and primary spermatocytes is critical to understand the processes which occur prior to meiosis during normal spermatogenesis. The genome-wide expression profiles of mouse type B spermatogonia and primary spermatocytes were investigated using the Solexa/Illumina digital gene expression (DGE) system, a tag based high-throughput transcriptome sequencing method, and the developmental processes which occur during early spermatogenesis were systematically analyzed. Gene expression patterns vary significantly between mouse type B spermatogonia and primary spermatocytes. The functional analysis revealed that genes related to junction assembly, regulation of the actin cytoskeleton and pluripotency were most significantly differently expressed. Pathway analysis indicated that the Wnt non-canonical signaling pathway played a central role and interacted with the actin filament organization pathway during the development of spermatogonia. This study provides a foundation for further analysis of the gene expression patterns and signaling pathways which regulate the molecular mechanisms of early spermatogenesis. Public Library of Science 2013-03-15 /pmc/articles/PMC3598852/ /pubmed/23554914 http://dx.doi.org/10.1371/journal.pone.0058680 Text en © 2013 Zhang et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Zhang, Xiujun
Hao, Lili
Meng, Lijun
Liu, Meiling
Zhao, Lina
Hu, Fen
Ding, Cunbao
Wang, Yang
He, Baoling
Pan, Yuxin
Fang, Wei
Chen, Jing
Hu, Songnian
Jia, Mengchun
Digital Gene Expression Tag Profiling Analysis of the Gene Expression Patterns Regulating the Early Stage of Mouse Spermatogenesis
title Digital Gene Expression Tag Profiling Analysis of the Gene Expression Patterns Regulating the Early Stage of Mouse Spermatogenesis
title_full Digital Gene Expression Tag Profiling Analysis of the Gene Expression Patterns Regulating the Early Stage of Mouse Spermatogenesis
title_fullStr Digital Gene Expression Tag Profiling Analysis of the Gene Expression Patterns Regulating the Early Stage of Mouse Spermatogenesis
title_full_unstemmed Digital Gene Expression Tag Profiling Analysis of the Gene Expression Patterns Regulating the Early Stage of Mouse Spermatogenesis
title_short Digital Gene Expression Tag Profiling Analysis of the Gene Expression Patterns Regulating the Early Stage of Mouse Spermatogenesis
title_sort digital gene expression tag profiling analysis of the gene expression patterns regulating the early stage of mouse spermatogenesis
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3598852/
https://www.ncbi.nlm.nih.gov/pubmed/23554914
http://dx.doi.org/10.1371/journal.pone.0058680
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