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Association of plasma endotoxin, inflammatory cytokines and risk of colorectal adenomas

BACKGROUND: Recent studies suggest that bacterial endotoxins may be associated with various chronic diseases, including colorectal adenomas and cancer. Given the evidence linking inflammation and colorectal cancer, we sought to determine if plasma endotoxin concentrations are associated with indicat...

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Autores principales: Kang, Melissa, Edmundson, Patrick, Araujo-Perez, Felix, McCoy, Amber N, Galanko, Joseph, Keku, Temitope O
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3599094/
https://www.ncbi.nlm.nih.gov/pubmed/23442743
http://dx.doi.org/10.1186/1471-2407-13-91
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author Kang, Melissa
Edmundson, Patrick
Araujo-Perez, Felix
McCoy, Amber N
Galanko, Joseph
Keku, Temitope O
author_facet Kang, Melissa
Edmundson, Patrick
Araujo-Perez, Felix
McCoy, Amber N
Galanko, Joseph
Keku, Temitope O
author_sort Kang, Melissa
collection PubMed
description BACKGROUND: Recent studies suggest that bacterial endotoxins may be associated with various chronic diseases, including colorectal adenomas and cancer. Given the evidence linking inflammation and colorectal cancer, we sought to determine if plasma endotoxin concentrations are associated with indicators of systemic or local inflammation and colorectal adenomas. METHODS: This cross-sectional study consisted of participants who underwent screening colonoscopies and included adenoma cases (n=138) and non-adenoma controls (n=324). Plasma concentrations of endotoxin were measured with Limulus Amebocyte Lysate (LAL) assay. We quantified concentrations of inflammatory cytokines, interleukin-4 (IL-4), IL-6, IL-8, IL-10, IL-12, tumor necrosis factor-alpha (TNF-α), and interferon-γ (IFN-γ) in plasma by ELISA and mRNA expression levels in rectal mucosal biopsies by quantitative RT-PCR. Interleukin-17 was evaluated only in the rectal mucosa. RESULTS: Compared to subjects with low plasma endotoxin concentrations, those with higher concentrations were more likely to have adenomas (OR 1.4, 95% CI 1.0-2.1). Among subjects with adenomas, those with villous histology were more likely to have higher endotoxin concentrations (5.4 vs. 4.1EU/mL, p=0.05) and lower plasma IFN-γ (0 vs. 1.64 pg/mL, p=0.02) compared to those with only tubular adenomas. Cases showed a trend of having higher plasma TNF-α levels than controls (p=0.06), but none of the other plasma or rectal mucosal cytokine levels differed between cases and controls. Elevated mucosal IL-12 levels were associated with having multiple adenomas (p=0.04). Higher concentrations of plasma endotoxin predicted increased plasma IL-12 levels (OR 1.5, 95% CI 1.0-2.2) and rectal mucosal IL-12 (OR 1.9, 95% CI 1.0-3.7) and IL-17 gene expression (OR 2.2, 95% CI 1.0-4.6). CONCLUSIONS: These findings suggest that interactions between elevated plasma endotoxin concentrations and inflammatory cytokines may be relevant to the development of colorectal adenomas.
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spelling pubmed-35990942013-03-17 Association of plasma endotoxin, inflammatory cytokines and risk of colorectal adenomas Kang, Melissa Edmundson, Patrick Araujo-Perez, Felix McCoy, Amber N Galanko, Joseph Keku, Temitope O BMC Cancer Research Article BACKGROUND: Recent studies suggest that bacterial endotoxins may be associated with various chronic diseases, including colorectal adenomas and cancer. Given the evidence linking inflammation and colorectal cancer, we sought to determine if plasma endotoxin concentrations are associated with indicators of systemic or local inflammation and colorectal adenomas. METHODS: This cross-sectional study consisted of participants who underwent screening colonoscopies and included adenoma cases (n=138) and non-adenoma controls (n=324). Plasma concentrations of endotoxin were measured with Limulus Amebocyte Lysate (LAL) assay. We quantified concentrations of inflammatory cytokines, interleukin-4 (IL-4), IL-6, IL-8, IL-10, IL-12, tumor necrosis factor-alpha (TNF-α), and interferon-γ (IFN-γ) in plasma by ELISA and mRNA expression levels in rectal mucosal biopsies by quantitative RT-PCR. Interleukin-17 was evaluated only in the rectal mucosa. RESULTS: Compared to subjects with low plasma endotoxin concentrations, those with higher concentrations were more likely to have adenomas (OR 1.4, 95% CI 1.0-2.1). Among subjects with adenomas, those with villous histology were more likely to have higher endotoxin concentrations (5.4 vs. 4.1EU/mL, p=0.05) and lower plasma IFN-γ (0 vs. 1.64 pg/mL, p=0.02) compared to those with only tubular adenomas. Cases showed a trend of having higher plasma TNF-α levels than controls (p=0.06), but none of the other plasma or rectal mucosal cytokine levels differed between cases and controls. Elevated mucosal IL-12 levels were associated with having multiple adenomas (p=0.04). Higher concentrations of plasma endotoxin predicted increased plasma IL-12 levels (OR 1.5, 95% CI 1.0-2.2) and rectal mucosal IL-12 (OR 1.9, 95% CI 1.0-3.7) and IL-17 gene expression (OR 2.2, 95% CI 1.0-4.6). CONCLUSIONS: These findings suggest that interactions between elevated plasma endotoxin concentrations and inflammatory cytokines may be relevant to the development of colorectal adenomas. BioMed Central 2013-02-26 /pmc/articles/PMC3599094/ /pubmed/23442743 http://dx.doi.org/10.1186/1471-2407-13-91 Text en Copyright ©2013 Kang et al; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Kang, Melissa
Edmundson, Patrick
Araujo-Perez, Felix
McCoy, Amber N
Galanko, Joseph
Keku, Temitope O
Association of plasma endotoxin, inflammatory cytokines and risk of colorectal adenomas
title Association of plasma endotoxin, inflammatory cytokines and risk of colorectal adenomas
title_full Association of plasma endotoxin, inflammatory cytokines and risk of colorectal adenomas
title_fullStr Association of plasma endotoxin, inflammatory cytokines and risk of colorectal adenomas
title_full_unstemmed Association of plasma endotoxin, inflammatory cytokines and risk of colorectal adenomas
title_short Association of plasma endotoxin, inflammatory cytokines and risk of colorectal adenomas
title_sort association of plasma endotoxin, inflammatory cytokines and risk of colorectal adenomas
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3599094/
https://www.ncbi.nlm.nih.gov/pubmed/23442743
http://dx.doi.org/10.1186/1471-2407-13-91
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