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Acute exercise decreases PTP-1B protein level and improves insulin signaling in the liver of old rats
It is now commonly accepted that chronic inflammation associated with obesity during aging induces insulin resistance in the liver. In the present study, we investigated whether the improvement in insulin sensitivity and insulin signaling, mediated by acute exercise, could be associated with modulat...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2013
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3599175/ https://www.ncbi.nlm.nih.gov/pubmed/23442260 http://dx.doi.org/10.1186/1742-4933-10-8 |
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author | de Moura, Leandro Pereira Souza Pauli, Luciana Santos Cintra, Dennys Esper de Souza, Claudio Teodoro da Silva, Adelino Sanchez Ramos Marinho, Rodolfo de Melo, Maria Alice Rostom Ropelle, Eduardo Rochete Pauli, José Rodrigo |
author_facet | de Moura, Leandro Pereira Souza Pauli, Luciana Santos Cintra, Dennys Esper de Souza, Claudio Teodoro da Silva, Adelino Sanchez Ramos Marinho, Rodolfo de Melo, Maria Alice Rostom Ropelle, Eduardo Rochete Pauli, José Rodrigo |
author_sort | de Moura, Leandro Pereira |
collection | PubMed |
description | It is now commonly accepted that chronic inflammation associated with obesity during aging induces insulin resistance in the liver. In the present study, we investigated whether the improvement in insulin sensitivity and insulin signaling, mediated by acute exercise, could be associated with modulation of protein-tyrosine phosphatase 1B (PTP-1B) in the liver of old rats. Aging rats were subjected to swimming for two 1.5-h long bouts, separated by a 45 min rest period. Sixteen hours after the exercise, the rats were sacrificed and proteins from the insulin signaling pathway were analyzed by immunoblotting. Our results show that the fat mass was increased in old rats. The reduction in glucose disappearance rate (Kitt) observed in aged rats was restored 16 h after exercise. Aging increased the content of PTP-1B and attenuated insulin signaling in the liver of rats, a phenomenon that was reversed by exercise. Aging rats also increased the IRβ/PTP-1B and IRS-1/PTP-1B association in the liver when compared with young rats. Conversely, in the liver of exercised old rats, IRβ/PTP-1B and IRS-1/PTP-1B association was markedly decreased. Moreover, in the hepatic tissue of old rats, the insulin signalling was decreased and PEPCK and G6Pase levels were increased when compared with young rats. Interestingly, 16 h after acute exercise, the PEPCK and G6Pase protein level were decreased in the old exercised group. These results provide new insights into the mechanisms by which exercise restores insulin signalling in liver during aging. |
format | Online Article Text |
id | pubmed-3599175 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2013 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-35991752013-03-17 Acute exercise decreases PTP-1B protein level and improves insulin signaling in the liver of old rats de Moura, Leandro Pereira Souza Pauli, Luciana Santos Cintra, Dennys Esper de Souza, Claudio Teodoro da Silva, Adelino Sanchez Ramos Marinho, Rodolfo de Melo, Maria Alice Rostom Ropelle, Eduardo Rochete Pauli, José Rodrigo Immun Ageing Research It is now commonly accepted that chronic inflammation associated with obesity during aging induces insulin resistance in the liver. In the present study, we investigated whether the improvement in insulin sensitivity and insulin signaling, mediated by acute exercise, could be associated with modulation of protein-tyrosine phosphatase 1B (PTP-1B) in the liver of old rats. Aging rats were subjected to swimming for two 1.5-h long bouts, separated by a 45 min rest period. Sixteen hours after the exercise, the rats were sacrificed and proteins from the insulin signaling pathway were analyzed by immunoblotting. Our results show that the fat mass was increased in old rats. The reduction in glucose disappearance rate (Kitt) observed in aged rats was restored 16 h after exercise. Aging increased the content of PTP-1B and attenuated insulin signaling in the liver of rats, a phenomenon that was reversed by exercise. Aging rats also increased the IRβ/PTP-1B and IRS-1/PTP-1B association in the liver when compared with young rats. Conversely, in the liver of exercised old rats, IRβ/PTP-1B and IRS-1/PTP-1B association was markedly decreased. Moreover, in the hepatic tissue of old rats, the insulin signalling was decreased and PEPCK and G6Pase levels were increased when compared with young rats. Interestingly, 16 h after acute exercise, the PEPCK and G6Pase protein level were decreased in the old exercised group. These results provide new insights into the mechanisms by which exercise restores insulin signalling in liver during aging. BioMed Central 2013-02-25 /pmc/articles/PMC3599175/ /pubmed/23442260 http://dx.doi.org/10.1186/1742-4933-10-8 Text en Copyright ©2013 Moura et al; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research de Moura, Leandro Pereira Souza Pauli, Luciana Santos Cintra, Dennys Esper de Souza, Claudio Teodoro da Silva, Adelino Sanchez Ramos Marinho, Rodolfo de Melo, Maria Alice Rostom Ropelle, Eduardo Rochete Pauli, José Rodrigo Acute exercise decreases PTP-1B protein level and improves insulin signaling in the liver of old rats |
title | Acute exercise decreases PTP-1B protein level and improves insulin signaling in the liver of old rats |
title_full | Acute exercise decreases PTP-1B protein level and improves insulin signaling in the liver of old rats |
title_fullStr | Acute exercise decreases PTP-1B protein level and improves insulin signaling in the liver of old rats |
title_full_unstemmed | Acute exercise decreases PTP-1B protein level and improves insulin signaling in the liver of old rats |
title_short | Acute exercise decreases PTP-1B protein level and improves insulin signaling in the liver of old rats |
title_sort | acute exercise decreases ptp-1b protein level and improves insulin signaling in the liver of old rats |
topic | Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3599175/ https://www.ncbi.nlm.nih.gov/pubmed/23442260 http://dx.doi.org/10.1186/1742-4933-10-8 |
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