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Comparative analysis of circulating dendritic cell subsets in patients with atopic diseases and sarcoidosis

BACKGROUND: Dendritic cells (DCs) are professional antigen-presenting cells that play a crucial role in the initiation and modulation of immune responses. Human circulating blood DCs are divided into two major subsets: myeloid DCs (mDCs); and plasmacytoid DCs (pDCs). Furthermore, mDCs are subdivided...

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Autores principales: Hayashi, Yumeko, Ishii, Yoshiki, Hata-Suzuki, Mitsumi, Arai, Ryo, Chibana, Kazuyuki, Takemasa, Akihiro, Fukuda, Takeshi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3599330/
https://www.ncbi.nlm.nih.gov/pubmed/23497225
http://dx.doi.org/10.1186/1465-9921-14-29
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author Hayashi, Yumeko
Ishii, Yoshiki
Hata-Suzuki, Mitsumi
Arai, Ryo
Chibana, Kazuyuki
Takemasa, Akihiro
Fukuda, Takeshi
author_facet Hayashi, Yumeko
Ishii, Yoshiki
Hata-Suzuki, Mitsumi
Arai, Ryo
Chibana, Kazuyuki
Takemasa, Akihiro
Fukuda, Takeshi
author_sort Hayashi, Yumeko
collection PubMed
description BACKGROUND: Dendritic cells (DCs) are professional antigen-presenting cells that play a crucial role in the initiation and modulation of immune responses. Human circulating blood DCs are divided into two major subsets: myeloid DCs (mDCs); and plasmacytoid DCs (pDCs). Furthermore, mDCs are subdivided into two subsets: Th1-promoting mDCs (mDC1s); and Th2-promoting mDCs (mDC2s). Although CD1a, CD1c, and CD141 are generally used for classifying mDC subsets, their adequacy as a specific marker remains unclear. We performed this study to compare circulating mDC, pDC, mDC1, and mDC2 subsets between Th1- and Th2-mediated diseases using CD1a and CD141, and to analyze the adequacy of CD1a and CD141 as a marker for mDC1s and mDC2s, respectively. METHODS: Thirty patients with sarcoidosis, 23 patients with atopic diseases, such as atopic bronchial asthma, and 23 healthy subjects as controls were enrolled in this study. Peripheral blood DC subsets were analyzed with flow cytometry according to expressions of CD11c, CD123, CD1a, and CD141. For functional analysis, we measured interleukin (IL) 12p40 levels produced by the sorted mDC subsets. RESULTS: The sarcoidosis group showed decreased total DC (P < 0.05) and mDC counts (P < 0.05) compared to controls. The atopy group showed decreased CD1a(+)mDC count (P < 0.05), and increased CD1a(-)mDC count (P < 0.05) compared to controls. CD141(+)mDC count in the atopy group was higher than controls (P < 0.05). Sorted CD1a(+)mDCs produced higher levels of IL-12p40 than CD1a(-)mDCs (P = 0.025) and CD141(+)mDCs (P = 0.018). CONCLUSIONS: We conclude that decreased count of CD1a(+)mDC and increased count of CD141(+)mDC may reflect the Th2-skewed immunity in atopic diseases. The results of IL-12 levels produced by the sorted mDC subsets suggested the adequacy of CD1a and CD141 as a marker for mDC1 and mDC2, respectively, in vivo.
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spelling pubmed-35993302013-03-17 Comparative analysis of circulating dendritic cell subsets in patients with atopic diseases and sarcoidosis Hayashi, Yumeko Ishii, Yoshiki Hata-Suzuki, Mitsumi Arai, Ryo Chibana, Kazuyuki Takemasa, Akihiro Fukuda, Takeshi Respir Res Research BACKGROUND: Dendritic cells (DCs) are professional antigen-presenting cells that play a crucial role in the initiation and modulation of immune responses. Human circulating blood DCs are divided into two major subsets: myeloid DCs (mDCs); and plasmacytoid DCs (pDCs). Furthermore, mDCs are subdivided into two subsets: Th1-promoting mDCs (mDC1s); and Th2-promoting mDCs (mDC2s). Although CD1a, CD1c, and CD141 are generally used for classifying mDC subsets, their adequacy as a specific marker remains unclear. We performed this study to compare circulating mDC, pDC, mDC1, and mDC2 subsets between Th1- and Th2-mediated diseases using CD1a and CD141, and to analyze the adequacy of CD1a and CD141 as a marker for mDC1s and mDC2s, respectively. METHODS: Thirty patients with sarcoidosis, 23 patients with atopic diseases, such as atopic bronchial asthma, and 23 healthy subjects as controls were enrolled in this study. Peripheral blood DC subsets were analyzed with flow cytometry according to expressions of CD11c, CD123, CD1a, and CD141. For functional analysis, we measured interleukin (IL) 12p40 levels produced by the sorted mDC subsets. RESULTS: The sarcoidosis group showed decreased total DC (P < 0.05) and mDC counts (P < 0.05) compared to controls. The atopy group showed decreased CD1a(+)mDC count (P < 0.05), and increased CD1a(-)mDC count (P < 0.05) compared to controls. CD141(+)mDC count in the atopy group was higher than controls (P < 0.05). Sorted CD1a(+)mDCs produced higher levels of IL-12p40 than CD1a(-)mDCs (P = 0.025) and CD141(+)mDCs (P = 0.018). CONCLUSIONS: We conclude that decreased count of CD1a(+)mDC and increased count of CD141(+)mDC may reflect the Th2-skewed immunity in atopic diseases. The results of IL-12 levels produced by the sorted mDC subsets suggested the adequacy of CD1a and CD141 as a marker for mDC1 and mDC2, respectively, in vivo. BioMed Central 2013 2013-03-04 /pmc/articles/PMC3599330/ /pubmed/23497225 http://dx.doi.org/10.1186/1465-9921-14-29 Text en Copyright ©2013 Hayashi et al; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research
Hayashi, Yumeko
Ishii, Yoshiki
Hata-Suzuki, Mitsumi
Arai, Ryo
Chibana, Kazuyuki
Takemasa, Akihiro
Fukuda, Takeshi
Comparative analysis of circulating dendritic cell subsets in patients with atopic diseases and sarcoidosis
title Comparative analysis of circulating dendritic cell subsets in patients with atopic diseases and sarcoidosis
title_full Comparative analysis of circulating dendritic cell subsets in patients with atopic diseases and sarcoidosis
title_fullStr Comparative analysis of circulating dendritic cell subsets in patients with atopic diseases and sarcoidosis
title_full_unstemmed Comparative analysis of circulating dendritic cell subsets in patients with atopic diseases and sarcoidosis
title_short Comparative analysis of circulating dendritic cell subsets in patients with atopic diseases and sarcoidosis
title_sort comparative analysis of circulating dendritic cell subsets in patients with atopic diseases and sarcoidosis
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3599330/
https://www.ncbi.nlm.nih.gov/pubmed/23497225
http://dx.doi.org/10.1186/1465-9921-14-29
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