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A systematic evaluation of miRNA:mRNA interactions involved in the migration and invasion of breast cancer cells

In this study we performed a systematic evaluation of functional miRNA-mRNA interactions associated with the invasiveness of breast cancer cells using a combination of integrated miRNA and mRNA expression profiling, bioinformatics prediction, and functional assays. Analysis of the miRNA expression i...

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Autores principales: Luo, Daya, Wilson, James M, Harvel, Nikki, Liu, Jimei, Pei, Lirong, Huang, Shuang, Hawthorn, LesleyAnn, Shi, Huidong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3599769/
https://www.ncbi.nlm.nih.gov/pubmed/23497265
http://dx.doi.org/10.1186/1479-5876-11-57
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author Luo, Daya
Wilson, James M
Harvel, Nikki
Liu, Jimei
Pei, Lirong
Huang, Shuang
Hawthorn, LesleyAnn
Shi, Huidong
author_facet Luo, Daya
Wilson, James M
Harvel, Nikki
Liu, Jimei
Pei, Lirong
Huang, Shuang
Hawthorn, LesleyAnn
Shi, Huidong
author_sort Luo, Daya
collection PubMed
description In this study we performed a systematic evaluation of functional miRNA-mRNA interactions associated with the invasiveness of breast cancer cells using a combination of integrated miRNA and mRNA expression profiling, bioinformatics prediction, and functional assays. Analysis of the miRNA expression identified 11 miRNAs that were differentially expressed, including 7 down-regulated (miR-200c, miR-205, miR-203, miR-141, miR-34a, miR-183, and miR-375) and 4 up-regulated miRNAs (miR-146a, miR-138, miR-125b1 and miR-100), in invasive cell lines when compared to normal and less invasive cell lines. Transfection of miR-200c, miR-205, and miR-375 mimics into MDA-MB-231 cells led to the inhibition of in vitro cell migration and invasion. The integrated analysis of miRNA and mRNA expression identified 35 known and novel target genes of miR-200c, miR-205, and mir-375, including CFL2, LAMC1, TIMP2, ZEB1, CDH11, PRKCA, PTPRJ, PTPRM, LDHB, and SEC23A. Surprisingly, the majority of these genes (27 genes) were target genes of miR-200c, suggesting that miR-200c plays a pivotal role in regulating the invasiveness of breast cancer cells. We characterized one of the target genes of miR-200c, CFL2, and demonstrated that CFL2 is overexpressed in aggressive breast cancer cell lines and can be significantly down-regulated by exogenous miR-200c. Tissue microarray analysis further revealed that CFL2 expression in primary breast cancer tissue correlated with tumor grade. The results obtained from this study may improve our understanding of the role of these candidate miRNAs and their target genes in relation to breast cancer invasiveness and ultimately lead to the identification of novel biomarkers associated with prognosis.
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spelling pubmed-35997692013-03-17 A systematic evaluation of miRNA:mRNA interactions involved in the migration and invasion of breast cancer cells Luo, Daya Wilson, James M Harvel, Nikki Liu, Jimei Pei, Lirong Huang, Shuang Hawthorn, LesleyAnn Shi, Huidong J Transl Med Research In this study we performed a systematic evaluation of functional miRNA-mRNA interactions associated with the invasiveness of breast cancer cells using a combination of integrated miRNA and mRNA expression profiling, bioinformatics prediction, and functional assays. Analysis of the miRNA expression identified 11 miRNAs that were differentially expressed, including 7 down-regulated (miR-200c, miR-205, miR-203, miR-141, miR-34a, miR-183, and miR-375) and 4 up-regulated miRNAs (miR-146a, miR-138, miR-125b1 and miR-100), in invasive cell lines when compared to normal and less invasive cell lines. Transfection of miR-200c, miR-205, and miR-375 mimics into MDA-MB-231 cells led to the inhibition of in vitro cell migration and invasion. The integrated analysis of miRNA and mRNA expression identified 35 known and novel target genes of miR-200c, miR-205, and mir-375, including CFL2, LAMC1, TIMP2, ZEB1, CDH11, PRKCA, PTPRJ, PTPRM, LDHB, and SEC23A. Surprisingly, the majority of these genes (27 genes) were target genes of miR-200c, suggesting that miR-200c plays a pivotal role in regulating the invasiveness of breast cancer cells. We characterized one of the target genes of miR-200c, CFL2, and demonstrated that CFL2 is overexpressed in aggressive breast cancer cell lines and can be significantly down-regulated by exogenous miR-200c. Tissue microarray analysis further revealed that CFL2 expression in primary breast cancer tissue correlated with tumor grade. The results obtained from this study may improve our understanding of the role of these candidate miRNAs and their target genes in relation to breast cancer invasiveness and ultimately lead to the identification of novel biomarkers associated with prognosis. BioMed Central 2013-03-05 /pmc/articles/PMC3599769/ /pubmed/23497265 http://dx.doi.org/10.1186/1479-5876-11-57 Text en Copyright ©2013 Luo et al; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License ( http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research
Luo, Daya
Wilson, James M
Harvel, Nikki
Liu, Jimei
Pei, Lirong
Huang, Shuang
Hawthorn, LesleyAnn
Shi, Huidong
A systematic evaluation of miRNA:mRNA interactions involved in the migration and invasion of breast cancer cells
title A systematic evaluation of miRNA:mRNA interactions involved in the migration and invasion of breast cancer cells
title_full A systematic evaluation of miRNA:mRNA interactions involved in the migration and invasion of breast cancer cells
title_fullStr A systematic evaluation of miRNA:mRNA interactions involved in the migration and invasion of breast cancer cells
title_full_unstemmed A systematic evaluation of miRNA:mRNA interactions involved in the migration and invasion of breast cancer cells
title_short A systematic evaluation of miRNA:mRNA interactions involved in the migration and invasion of breast cancer cells
title_sort systematic evaluation of mirna:mrna interactions involved in the migration and invasion of breast cancer cells
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3599769/
https://www.ncbi.nlm.nih.gov/pubmed/23497265
http://dx.doi.org/10.1186/1479-5876-11-57
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