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Mycobacterium avium subsp. paratuberculosis lipophilic antigen causes Crohn’s disease-type necrotizing colitis in Mice

Background: A 2,4,6-trinitrobenzene sulfonic acid (TNBS)-induced murine colitis model was developed to investigate the pathogenesis and to evaluate a method of treating human Crohn’s disease. This experimental model rapidly induces colitis similar to human Crohn’s disease lesion in a reproducible ma...

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Autores principales: Momotani, Eiichi, Ozaki, Hiroshi, Hori, Masatoshi, Yamamoto, Shizuo, Kuribayashi, Takashi, Eda, Shigetoshi, Ikegami, Masahiro
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer International Publishing AG 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3600126/
https://www.ncbi.nlm.nih.gov/pubmed/23519342
http://dx.doi.org/10.1186/2193-1801-1-47
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author Momotani, Eiichi
Ozaki, Hiroshi
Hori, Masatoshi
Yamamoto, Shizuo
Kuribayashi, Takashi
Eda, Shigetoshi
Ikegami, Masahiro
author_facet Momotani, Eiichi
Ozaki, Hiroshi
Hori, Masatoshi
Yamamoto, Shizuo
Kuribayashi, Takashi
Eda, Shigetoshi
Ikegami, Masahiro
author_sort Momotani, Eiichi
collection PubMed
description Background: A 2,4,6-trinitrobenzene sulfonic acid (TNBS)-induced murine colitis model was developed to investigate the pathogenesis and to evaluate a method of treating human Crohn’s disease. This experimental model rapidly induces colitis similar to human Crohn’s disease lesion in a reproducible manner. However, natural exposure of the human digestive tract to TNBS is unrealistic. A novel animal model based on realistic data is eagerly anticipated in future research on pathogenesis of CD. Method: We evaluated the potency of Map antigen molecules in an effort to develop a novel colitis model using a more realistic source than TNBS. We prepared the Map antigen by ethanol extraction and developed a mouse model in a manner similar to that of the well-known TNBS-induced colitis in mice. In the experiment, seven days after subcutaneous (SC) injection of the antigen into normal C57BL/6 mice, the same antigen in 50% ethanol was injected into the colon by the transanal route with a fine cannula. Results: On the fifth day after the transanal injection, histopathological examination revealed full-thickness necrotizing colitis with erosion and ulcers; severe infiltration with neutrophils, lymphocytes, macrophages, and perforation. However, no change was detected with each single Map-antigen injection. Conclusion: The present results provide a novel animal model for research on CD and may be the key to clarifying the relationship between CD and Map. This is the first evidence that mycobacterium antigen induces necrotizing colitis.
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spelling pubmed-36001262013-03-19 Mycobacterium avium subsp. paratuberculosis lipophilic antigen causes Crohn’s disease-type necrotizing colitis in Mice Momotani, Eiichi Ozaki, Hiroshi Hori, Masatoshi Yamamoto, Shizuo Kuribayashi, Takashi Eda, Shigetoshi Ikegami, Masahiro Springerplus Research Background: A 2,4,6-trinitrobenzene sulfonic acid (TNBS)-induced murine colitis model was developed to investigate the pathogenesis and to evaluate a method of treating human Crohn’s disease. This experimental model rapidly induces colitis similar to human Crohn’s disease lesion in a reproducible manner. However, natural exposure of the human digestive tract to TNBS is unrealistic. A novel animal model based on realistic data is eagerly anticipated in future research on pathogenesis of CD. Method: We evaluated the potency of Map antigen molecules in an effort to develop a novel colitis model using a more realistic source than TNBS. We prepared the Map antigen by ethanol extraction and developed a mouse model in a manner similar to that of the well-known TNBS-induced colitis in mice. In the experiment, seven days after subcutaneous (SC) injection of the antigen into normal C57BL/6 mice, the same antigen in 50% ethanol was injected into the colon by the transanal route with a fine cannula. Results: On the fifth day after the transanal injection, histopathological examination revealed full-thickness necrotizing colitis with erosion and ulcers; severe infiltration with neutrophils, lymphocytes, macrophages, and perforation. However, no change was detected with each single Map-antigen injection. Conclusion: The present results provide a novel animal model for research on CD and may be the key to clarifying the relationship between CD and Map. This is the first evidence that mycobacterium antigen induces necrotizing colitis. Springer International Publishing AG 2012-11-08 /pmc/articles/PMC3600126/ /pubmed/23519342 http://dx.doi.org/10.1186/2193-1801-1-47 Text en © Momotani et al.; licensee Springer. 2012 This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research
Momotani, Eiichi
Ozaki, Hiroshi
Hori, Masatoshi
Yamamoto, Shizuo
Kuribayashi, Takashi
Eda, Shigetoshi
Ikegami, Masahiro
Mycobacterium avium subsp. paratuberculosis lipophilic antigen causes Crohn’s disease-type necrotizing colitis in Mice
title Mycobacterium avium subsp. paratuberculosis lipophilic antigen causes Crohn’s disease-type necrotizing colitis in Mice
title_full Mycobacterium avium subsp. paratuberculosis lipophilic antigen causes Crohn’s disease-type necrotizing colitis in Mice
title_fullStr Mycobacterium avium subsp. paratuberculosis lipophilic antigen causes Crohn’s disease-type necrotizing colitis in Mice
title_full_unstemmed Mycobacterium avium subsp. paratuberculosis lipophilic antigen causes Crohn’s disease-type necrotizing colitis in Mice
title_short Mycobacterium avium subsp. paratuberculosis lipophilic antigen causes Crohn’s disease-type necrotizing colitis in Mice
title_sort mycobacterium avium subsp. paratuberculosis lipophilic antigen causes crohn’s disease-type necrotizing colitis in mice
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3600126/
https://www.ncbi.nlm.nih.gov/pubmed/23519342
http://dx.doi.org/10.1186/2193-1801-1-47
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