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The Aquatic Environment as a Reservoir of Vibrio cholerae O1 in Hydrographic Basins of the State of Pernambuco, Brazil

After the worldwide cholera epidemic in 1993, permanent environmental monitoring of hydrographic basins was established in Pernambuco, Brazil, where cholera is endemic. After a quiescent period, 4 rfbN (serogroup O1) positive water samples that were culture negative were detected by multiplex single...

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Autores principales: Mendes-Marques, Carina Lucena, Silveira Filho, Vladimir da Mota, da Costa, Ana Paula Rocha, Nunes, Mariana de Lira, da Silva Filho, Sandoval Vieira, Figueirôa, Ângela Cristina Torres de Araújo, Hofer, Ernesto, de Almeida, Alzira Maria Paiva, Leal, Nilma Cintra
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi Publishing Corporation 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3600343/
https://www.ncbi.nlm.nih.gov/pubmed/23533357
http://dx.doi.org/10.1155/2013/746254
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author Mendes-Marques, Carina Lucena
Silveira Filho, Vladimir da Mota
da Costa, Ana Paula Rocha
Nunes, Mariana de Lira
da Silva Filho, Sandoval Vieira
Figueirôa, Ângela Cristina Torres de Araújo
Hofer, Ernesto
de Almeida, Alzira Maria Paiva
Leal, Nilma Cintra
author_facet Mendes-Marques, Carina Lucena
Silveira Filho, Vladimir da Mota
da Costa, Ana Paula Rocha
Nunes, Mariana de Lira
da Silva Filho, Sandoval Vieira
Figueirôa, Ângela Cristina Torres de Araújo
Hofer, Ernesto
de Almeida, Alzira Maria Paiva
Leal, Nilma Cintra
author_sort Mendes-Marques, Carina Lucena
collection PubMed
description After the worldwide cholera epidemic in 1993, permanent environmental monitoring of hydrographic basins was established in Pernambuco, Brazil, where cholera is endemic. After a quiescent period, 4 rfbN (serogroup O1) positive water samples that were culture negative were detected by multiplex single-tube nested PCR (MSTNPCR); 2 of these were also ctxA (cholera toxin) positive. From May to June 2012, 30 V. cholerae O1 isolates were obtained by culturing samples. These isolates were analyzed for the presence of virulence genes by PCR, intergenic spacer region 16S-23S PCR (ISR-PCR), and pulsed field gel electrophoresis (PFGE). The isolates were positive for the rfbN gene and negative for the assessed pathogenic genes and were classified into 2 groups by ISR and the same profile by PFGE. Close genetic similarity was observed between them (2012) and environmental strains from 2004 to 2005, indicating the permanence of endemic V. cholerae O1 in the region.
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spelling pubmed-36003432013-03-26 The Aquatic Environment as a Reservoir of Vibrio cholerae O1 in Hydrographic Basins of the State of Pernambuco, Brazil Mendes-Marques, Carina Lucena Silveira Filho, Vladimir da Mota da Costa, Ana Paula Rocha Nunes, Mariana de Lira da Silva Filho, Sandoval Vieira Figueirôa, Ângela Cristina Torres de Araújo Hofer, Ernesto de Almeida, Alzira Maria Paiva Leal, Nilma Cintra ScientificWorldJournal Research Article After the worldwide cholera epidemic in 1993, permanent environmental monitoring of hydrographic basins was established in Pernambuco, Brazil, where cholera is endemic. After a quiescent period, 4 rfbN (serogroup O1) positive water samples that were culture negative were detected by multiplex single-tube nested PCR (MSTNPCR); 2 of these were also ctxA (cholera toxin) positive. From May to June 2012, 30 V. cholerae O1 isolates were obtained by culturing samples. These isolates were analyzed for the presence of virulence genes by PCR, intergenic spacer region 16S-23S PCR (ISR-PCR), and pulsed field gel electrophoresis (PFGE). The isolates were positive for the rfbN gene and negative for the assessed pathogenic genes and were classified into 2 groups by ISR and the same profile by PFGE. Close genetic similarity was observed between them (2012) and environmental strains from 2004 to 2005, indicating the permanence of endemic V. cholerae O1 in the region. Hindawi Publishing Corporation 2013-02-25 /pmc/articles/PMC3600343/ /pubmed/23533357 http://dx.doi.org/10.1155/2013/746254 Text en Copyright © 2013 Carina Lucena Mendes-Marques et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Mendes-Marques, Carina Lucena
Silveira Filho, Vladimir da Mota
da Costa, Ana Paula Rocha
Nunes, Mariana de Lira
da Silva Filho, Sandoval Vieira
Figueirôa, Ângela Cristina Torres de Araújo
Hofer, Ernesto
de Almeida, Alzira Maria Paiva
Leal, Nilma Cintra
The Aquatic Environment as a Reservoir of Vibrio cholerae O1 in Hydrographic Basins of the State of Pernambuco, Brazil
title The Aquatic Environment as a Reservoir of Vibrio cholerae O1 in Hydrographic Basins of the State of Pernambuco, Brazil
title_full The Aquatic Environment as a Reservoir of Vibrio cholerae O1 in Hydrographic Basins of the State of Pernambuco, Brazil
title_fullStr The Aquatic Environment as a Reservoir of Vibrio cholerae O1 in Hydrographic Basins of the State of Pernambuco, Brazil
title_full_unstemmed The Aquatic Environment as a Reservoir of Vibrio cholerae O1 in Hydrographic Basins of the State of Pernambuco, Brazil
title_short The Aquatic Environment as a Reservoir of Vibrio cholerae O1 in Hydrographic Basins of the State of Pernambuco, Brazil
title_sort aquatic environment as a reservoir of vibrio cholerae o1 in hydrographic basins of the state of pernambuco, brazil
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3600343/
https://www.ncbi.nlm.nih.gov/pubmed/23533357
http://dx.doi.org/10.1155/2013/746254
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