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Genetic Transformation of a Clinical (Genital Tract), Plasmid-Free Isolate of Chlamydia trachomatis: Engineering the Plasmid as a Cloning Vector

Our study had three objectives: to extend the plasmid-based transformation protocol to a clinical isolate of C. trachomatis belonging to the trachoma biovar, to provide “proof of principle” that it is possible to “knock out” selected plasmid genes (retaining a replication competent plasmid) and to i...

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Detalles Bibliográficos
Autores principales:	Wang, Yibing, Kahane, Simona, Cutcliffe, Lesley T., Skilton, Rachel J., Lambden, Paul R., Persson, Kenneth, Bjartling, Carina, Clarke, Ian N.
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Public Library of Science 2013
Materias:	Research Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3601068/ https://www.ncbi.nlm.nih.gov/pubmed/23527131 http://dx.doi.org/10.1371/journal.pone.0059195

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