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Vitamin E Ameliorates the Decremental Effect of Paraquat on Cardiomyocyte Contractility in Rats

BACKGROUND: Exposure to pesticides and industrial toxins are implicated in cardiovascular disease. Paraquat (PAR) is a toxic chemical widely used as an herbicide in developing countries and described as a major suicide agent. The hypothesis tested here is that PAR induced myocardial dysfunction may...

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Autores principales: Fahim, Mohamed Abdelmonem, Howarth, Frank Christopher, Nemmar, Abderrahim, Qureshi, Mohamed Anwar, Shafiullah, Mohamed, Jayaprakash, Petrilla, Hasan, Mohamed Yousif
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3601115/
https://www.ncbi.nlm.nih.gov/pubmed/23526948
http://dx.doi.org/10.1371/journal.pone.0057651
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author Fahim, Mohamed Abdelmonem
Howarth, Frank Christopher
Nemmar, Abderrahim
Qureshi, Mohamed Anwar
Shafiullah, Mohamed
Jayaprakash, Petrilla
Hasan, Mohamed Yousif
author_facet Fahim, Mohamed Abdelmonem
Howarth, Frank Christopher
Nemmar, Abderrahim
Qureshi, Mohamed Anwar
Shafiullah, Mohamed
Jayaprakash, Petrilla
Hasan, Mohamed Yousif
author_sort Fahim, Mohamed Abdelmonem
collection PubMed
description BACKGROUND: Exposure to pesticides and industrial toxins are implicated in cardiovascular disease. Paraquat (PAR) is a toxic chemical widely used as an herbicide in developing countries and described as a major suicide agent. The hypothesis tested here is that PAR induced myocardial dysfunction may be attributed to altered mechanisms of Ca(2+) transport which are in turn possibly linked to oxidative stress. The mechanisms of PAR induced myocardial dysfunction and the impact of antioxidant protection was investigated in rat ventricular myocytes. METHODOLOGY: Forty adult male Wistar rats were divided into 4 groups receiving the following daily intraperitoneal injections for 3 weeks: Group 1 PAR (10 mg/kg), Control Group 2 saline, Group 3 vitamin E (100 mg/kg) and Group 4 PAR (10 mg/kg) and vitamin E (100 mg/kg). Ventricular action potentials were measured in isolated perfused heart, shortening and intracellular Ca(2+) in electrically stimulated ventricular myocytes by video edge detection and fluorescence photometry techniques, and superoxide dismutase (SOD) and catalase (CAT) levels in heart tissue. PRINCIPAL FINDINGS: Spontaneous heart rate, resting cell length, time to peak (TPK) and time to half (THALF) relaxation of myocyte shortening were unaltered. Amplitude of shortening was significantly reduced in PAR treated rats (4.99±0.26%) and was normalized by vitamin E (7.46±0.44%) compared to controls (7.87±0.52%). PAR significantly increased myocytes resting intracellular Ca(2+) whilst TPK and THALF decay and amplitude of the Ca(2+) transient were unaltered. The fura-2–cell length trajectory during the relaxation of the twitch contraction was significantly altered in myocytes from PAR treated rats compared to controls suggesting altered myofilament sensitivity to Ca(2+) as it was normalized by vitamin E treatment. A significant increase in SOD and CAT activities was observed in both PAR and vitamin E plus PAR groups. CONCLUSIONS: PAR exposure compromised rats heart function and ameliorated by vitamin E treatment.
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spelling pubmed-36011152013-03-22 Vitamin E Ameliorates the Decremental Effect of Paraquat on Cardiomyocyte Contractility in Rats Fahim, Mohamed Abdelmonem Howarth, Frank Christopher Nemmar, Abderrahim Qureshi, Mohamed Anwar Shafiullah, Mohamed Jayaprakash, Petrilla Hasan, Mohamed Yousif PLoS One Research Article BACKGROUND: Exposure to pesticides and industrial toxins are implicated in cardiovascular disease. Paraquat (PAR) is a toxic chemical widely used as an herbicide in developing countries and described as a major suicide agent. The hypothesis tested here is that PAR induced myocardial dysfunction may be attributed to altered mechanisms of Ca(2+) transport which are in turn possibly linked to oxidative stress. The mechanisms of PAR induced myocardial dysfunction and the impact of antioxidant protection was investigated in rat ventricular myocytes. METHODOLOGY: Forty adult male Wistar rats were divided into 4 groups receiving the following daily intraperitoneal injections for 3 weeks: Group 1 PAR (10 mg/kg), Control Group 2 saline, Group 3 vitamin E (100 mg/kg) and Group 4 PAR (10 mg/kg) and vitamin E (100 mg/kg). Ventricular action potentials were measured in isolated perfused heart, shortening and intracellular Ca(2+) in electrically stimulated ventricular myocytes by video edge detection and fluorescence photometry techniques, and superoxide dismutase (SOD) and catalase (CAT) levels in heart tissue. PRINCIPAL FINDINGS: Spontaneous heart rate, resting cell length, time to peak (TPK) and time to half (THALF) relaxation of myocyte shortening were unaltered. Amplitude of shortening was significantly reduced in PAR treated rats (4.99±0.26%) and was normalized by vitamin E (7.46±0.44%) compared to controls (7.87±0.52%). PAR significantly increased myocytes resting intracellular Ca(2+) whilst TPK and THALF decay and amplitude of the Ca(2+) transient were unaltered. The fura-2–cell length trajectory during the relaxation of the twitch contraction was significantly altered in myocytes from PAR treated rats compared to controls suggesting altered myofilament sensitivity to Ca(2+) as it was normalized by vitamin E treatment. A significant increase in SOD and CAT activities was observed in both PAR and vitamin E plus PAR groups. CONCLUSIONS: PAR exposure compromised rats heart function and ameliorated by vitamin E treatment. Public Library of Science 2013-03-18 /pmc/articles/PMC3601115/ /pubmed/23526948 http://dx.doi.org/10.1371/journal.pone.0057651 Text en © 2013 Fahim et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Fahim, Mohamed Abdelmonem
Howarth, Frank Christopher
Nemmar, Abderrahim
Qureshi, Mohamed Anwar
Shafiullah, Mohamed
Jayaprakash, Petrilla
Hasan, Mohamed Yousif
Vitamin E Ameliorates the Decremental Effect of Paraquat on Cardiomyocyte Contractility in Rats
title Vitamin E Ameliorates the Decremental Effect of Paraquat on Cardiomyocyte Contractility in Rats
title_full Vitamin E Ameliorates the Decremental Effect of Paraquat on Cardiomyocyte Contractility in Rats
title_fullStr Vitamin E Ameliorates the Decremental Effect of Paraquat on Cardiomyocyte Contractility in Rats
title_full_unstemmed Vitamin E Ameliorates the Decremental Effect of Paraquat on Cardiomyocyte Contractility in Rats
title_short Vitamin E Ameliorates the Decremental Effect of Paraquat on Cardiomyocyte Contractility in Rats
title_sort vitamin e ameliorates the decremental effect of paraquat on cardiomyocyte contractility in rats
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3601115/
https://www.ncbi.nlm.nih.gov/pubmed/23526948
http://dx.doi.org/10.1371/journal.pone.0057651
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