Identification of antifungal principle in the solvent extract of an endophytic fungus Chaetomium globosum from Withania somnifera

Extracts of Chaetomium globosum EF18, isolated as endophytic fungus from Withania somnifera, were found effective against Sclerotinia sclerotiorum. Ethyl acetate and methanol extracts were more effective than hexane extract showing >80% growth inhibition. Bioactive compound (antibiotic Sch 210971...

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Autores principales: Kumar, Susheel, Kaushik, Nutan, Proksch, Peter
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer International Publishing AG 2013
Materias:
Research
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3601270/
https://www.ncbi.nlm.nih.gov/pubmed/23519550
http://dx.doi.org/10.1186/2193-1801-2-37
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author Kumar, Susheel
Kaushik, Nutan
Proksch, Peter
author_facet Kumar, Susheel
Kaushik, Nutan
Proksch, Peter
author_sort Kumar, Susheel
collection PubMed
description Extracts of Chaetomium globosum EF18, isolated as endophytic fungus from Withania somnifera, were found effective against Sclerotinia sclerotiorum. Ethyl acetate and methanol extracts were more effective than hexane extract showing >80% growth inhibition. Bioactive compound (antibiotic Sch 210971, m/z 445 and λ(max) 290) having antifungal activity against S. sclerotiorum has been isolated in pure form from the ethyl acetate extract following bioassay guided fractionation. Apart from this compound other fractions of polar to medium polarity were also found effective. Fraction no. VIII from VLC (Vacuum liquid chromatography) column of ethyl acetate extract was most active having IC(50) value 35.4 μg/ml.
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spelling pubmed-36012702013-03-19 Identification of antifungal principle in the solvent extract of an endophytic fungus Chaetomium globosum from Withania somnifera Kumar, Susheel Kaushik, Nutan Proksch, Peter Springerplus Research Extracts of Chaetomium globosum EF18, isolated as endophytic fungus from Withania somnifera, were found effective against Sclerotinia sclerotiorum. Ethyl acetate and methanol extracts were more effective than hexane extract showing >80% growth inhibition. Bioactive compound (antibiotic Sch 210971, m/z 445 and λ(max) 290) having antifungal activity against S. sclerotiorum has been isolated in pure form from the ethyl acetate extract following bioassay guided fractionation. Apart from this compound other fractions of polar to medium polarity were also found effective. Fraction no. VIII from VLC (Vacuum liquid chromatography) column of ethyl acetate extract was most active having IC(50) value 35.4 μg/ml. Springer International Publishing AG 2013-02-06 /pmc/articles/PMC3601270/ /pubmed/23519550 http://dx.doi.org/10.1186/2193-1801-2-37 Text en © Kumar et al; licensee Springer. 2013 This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research
Kumar, Susheel
Kaushik, Nutan
Proksch, Peter
Identification of antifungal principle in the solvent extract of an endophytic fungus Chaetomium globosum from Withania somnifera
title Identification of antifungal principle in the solvent extract of an endophytic fungus Chaetomium globosum from Withania somnifera
title_full Identification of antifungal principle in the solvent extract of an endophytic fungus Chaetomium globosum from Withania somnifera
title_fullStr Identification of antifungal principle in the solvent extract of an endophytic fungus Chaetomium globosum from Withania somnifera
title_full_unstemmed Identification of antifungal principle in the solvent extract of an endophytic fungus Chaetomium globosum from Withania somnifera
title_short Identification of antifungal principle in the solvent extract of an endophytic fungus Chaetomium globosum from Withania somnifera
title_sort identification of antifungal principle in the solvent extract of an endophytic fungus chaetomium globosum from withania somnifera
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3601270/
https://www.ncbi.nlm.nih.gov/pubmed/23519550
http://dx.doi.org/10.1186/2193-1801-2-37
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