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Sox10-dependent neural crest origin of olfactory microvillous neurons in zebrafish

The sense of smell in vertebrates is detected by specialized sensory neurons derived from the peripheral nervous system. Classically, it has been presumed that the olfactory placode forms all olfactory sensory neurons. In contrast, we show that the cranial neural crest is the primary source of micro...

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Autores principales: Saxena, Ankur, Peng, Brian N, Bronner, Marianne E
Formato: Online Artículo Texto
Lenguaje:English
Publicado: eLife Sciences Publications, Ltd 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3601810/
https://www.ncbi.nlm.nih.gov/pubmed/23539289
http://dx.doi.org/10.7554/eLife.00336
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author Saxena, Ankur
Peng, Brian N
Bronner, Marianne E
author_facet Saxena, Ankur
Peng, Brian N
Bronner, Marianne E
author_sort Saxena, Ankur
collection PubMed
description The sense of smell in vertebrates is detected by specialized sensory neurons derived from the peripheral nervous system. Classically, it has been presumed that the olfactory placode forms all olfactory sensory neurons. In contrast, we show that the cranial neural crest is the primary source of microvillous sensory neurons within the olfactory epithelium of zebrafish embryos. Using photoconversion-based fate mapping and live cell tracking coupled with laser ablation, we followed neural crest precursors as they migrated from the neural tube to the nasal cavity. A subset that coexpressed Sox10 protein and a neurogenin1 reporter ingressed into the olfactory epithelium and differentiated into microvillous sensory neurons. Timed loss-of-function analysis revealed a critical role for Sox10 in microvillous neurogenesis. Taken together, these findings directly demonstrate a heretofore unknown contribution of the cranial neural crest to olfactory sensory neurons in zebrafish and provide important insights into the assembly of the nascent olfactory system. DOI: http://dx.doi.org/10.7554/eLife.00336.001
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spelling pubmed-36018102013-03-28 Sox10-dependent neural crest origin of olfactory microvillous neurons in zebrafish Saxena, Ankur Peng, Brian N Bronner, Marianne E eLife Developmental Biology and Stem Cells The sense of smell in vertebrates is detected by specialized sensory neurons derived from the peripheral nervous system. Classically, it has been presumed that the olfactory placode forms all olfactory sensory neurons. In contrast, we show that the cranial neural crest is the primary source of microvillous sensory neurons within the olfactory epithelium of zebrafish embryos. Using photoconversion-based fate mapping and live cell tracking coupled with laser ablation, we followed neural crest precursors as they migrated from the neural tube to the nasal cavity. A subset that coexpressed Sox10 protein and a neurogenin1 reporter ingressed into the olfactory epithelium and differentiated into microvillous sensory neurons. Timed loss-of-function analysis revealed a critical role for Sox10 in microvillous neurogenesis. Taken together, these findings directly demonstrate a heretofore unknown contribution of the cranial neural crest to olfactory sensory neurons in zebrafish and provide important insights into the assembly of the nascent olfactory system. DOI: http://dx.doi.org/10.7554/eLife.00336.001 eLife Sciences Publications, Ltd 2013-03-19 /pmc/articles/PMC3601810/ /pubmed/23539289 http://dx.doi.org/10.7554/eLife.00336 Text en Copyright © 2013, Saxena et al http://creativecommons.org/licenses/by/3.0/ This article is distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0/) , which permits unrestricted use and redistribution provided that the original author and source are credited.
spellingShingle Developmental Biology and Stem Cells
Saxena, Ankur
Peng, Brian N
Bronner, Marianne E
Sox10-dependent neural crest origin of olfactory microvillous neurons in zebrafish
title Sox10-dependent neural crest origin of olfactory microvillous neurons in zebrafish
title_full Sox10-dependent neural crest origin of olfactory microvillous neurons in zebrafish
title_fullStr Sox10-dependent neural crest origin of olfactory microvillous neurons in zebrafish
title_full_unstemmed Sox10-dependent neural crest origin of olfactory microvillous neurons in zebrafish
title_short Sox10-dependent neural crest origin of olfactory microvillous neurons in zebrafish
title_sort sox10-dependent neural crest origin of olfactory microvillous neurons in zebrafish
topic Developmental Biology and Stem Cells
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3601810/
https://www.ncbi.nlm.nih.gov/pubmed/23539289
http://dx.doi.org/10.7554/eLife.00336
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