Cargando…

Addressing the Role of microRNAs in Reprogramming Leaf Growth during Drought Stress in Brachypodium distachyon

Plant responses to drought are regulated by complex genetic and epigenetic networks leading to rapid reprogramming of plant growth. miRNAs have been widely indicated as key players in the regulation of growth and development. The role of miRNAs in drought response was investigated in young leaves of...

Descripción completa

Detalles Bibliográficos
Autores principales: Bertolini, Edoardo, Verelst, Wim, Horner, David Stephen, Gianfranceschi, Luca, Piccolo, Viviana, Inzé, Dirk, Pè, Mario Enrico, Mica, Erica
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3603004/
https://www.ncbi.nlm.nih.gov/pubmed/23264558
http://dx.doi.org/10.1093/mp/sss160
_version_ 1782263611320696832
author Bertolini, Edoardo
Verelst, Wim
Horner, David Stephen
Gianfranceschi, Luca
Piccolo, Viviana
Inzé, Dirk
Pè, Mario Enrico
Mica, Erica
author_facet Bertolini, Edoardo
Verelst, Wim
Horner, David Stephen
Gianfranceschi, Luca
Piccolo, Viviana
Inzé, Dirk
Pè, Mario Enrico
Mica, Erica
author_sort Bertolini, Edoardo
collection PubMed
description Plant responses to drought are regulated by complex genetic and epigenetic networks leading to rapid reprogramming of plant growth. miRNAs have been widely indicated as key players in the regulation of growth and development. The role of miRNAs in drought response was investigated in young leaves of Brachypodium distachyon, a drought-tolerant monocot model species. Adopting an in vivo drought assay, shown to cause a dramatic reduction in leaf size, mostly due to reduced cell expansion, small RNA libraries were produced from proliferating and expanding leaf cells. Next-generation sequencing data were analyzed using an in-house bioinformatics pipeline allowing the identification of 66 annotated miRNA genes and 122 new high confidence predictions greatly expanding the number of known Brachypodium miRNAs. In addition, we identified four TAS3 loci and a large number of siRNA-producing loci that show characteristics suggesting that they may represent young miRNA genes. Most miRNAs showed a high expression level, consistent with their involvement in early leaf development and cell identity. Proliferating and expanding leaf cells respond differently to drought treatment and differential expression analyses suggest novel evidence for an miRNA regulatory network controlling cell division in both normal and stressed conditions and demonstrate that drought triggers a genetic reprogramming of leaf growth in which miRNAs are deeply involved.
format Online
Article
Text
id pubmed-3603004
institution National Center for Biotechnology Information
language English
publishDate 2013
publisher Oxford University Press
record_format MEDLINE/PubMed
spelling pubmed-36030042013-03-20 Addressing the Role of microRNAs in Reprogramming Leaf Growth during Drought Stress in Brachypodium distachyon Bertolini, Edoardo Verelst, Wim Horner, David Stephen Gianfranceschi, Luca Piccolo, Viviana Inzé, Dirk Pè, Mario Enrico Mica, Erica Mol Plant Research Article Plant responses to drought are regulated by complex genetic and epigenetic networks leading to rapid reprogramming of plant growth. miRNAs have been widely indicated as key players in the regulation of growth and development. The role of miRNAs in drought response was investigated in young leaves of Brachypodium distachyon, a drought-tolerant monocot model species. Adopting an in vivo drought assay, shown to cause a dramatic reduction in leaf size, mostly due to reduced cell expansion, small RNA libraries were produced from proliferating and expanding leaf cells. Next-generation sequencing data were analyzed using an in-house bioinformatics pipeline allowing the identification of 66 annotated miRNA genes and 122 new high confidence predictions greatly expanding the number of known Brachypodium miRNAs. In addition, we identified four TAS3 loci and a large number of siRNA-producing loci that show characteristics suggesting that they may represent young miRNA genes. Most miRNAs showed a high expression level, consistent with their involvement in early leaf development and cell identity. Proliferating and expanding leaf cells respond differently to drought treatment and differential expression analyses suggest novel evidence for an miRNA regulatory network controlling cell division in both normal and stressed conditions and demonstrate that drought triggers a genetic reprogramming of leaf growth in which miRNAs are deeply involved. Oxford University Press 2013-03 2012-12-21 /pmc/articles/PMC3603004/ /pubmed/23264558 http://dx.doi.org/10.1093/mp/sss160 Text en © The Author 2013. Published by the Molecular Plant Shanghai Editorial Office in association with Oxford University Press on behalf of CSPB and IPPE, SIBS, CAS. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by-nc/3.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com.
spellingShingle Research Article
Bertolini, Edoardo
Verelst, Wim
Horner, David Stephen
Gianfranceschi, Luca
Piccolo, Viviana
Inzé, Dirk
Pè, Mario Enrico
Mica, Erica
Addressing the Role of microRNAs in Reprogramming Leaf Growth during Drought Stress in Brachypodium distachyon
title Addressing the Role of microRNAs in Reprogramming Leaf Growth during Drought Stress in Brachypodium distachyon
title_full Addressing the Role of microRNAs in Reprogramming Leaf Growth during Drought Stress in Brachypodium distachyon
title_fullStr Addressing the Role of microRNAs in Reprogramming Leaf Growth during Drought Stress in Brachypodium distachyon
title_full_unstemmed Addressing the Role of microRNAs in Reprogramming Leaf Growth during Drought Stress in Brachypodium distachyon
title_short Addressing the Role of microRNAs in Reprogramming Leaf Growth during Drought Stress in Brachypodium distachyon
title_sort addressing the role of micrornas in reprogramming leaf growth during drought stress in brachypodium distachyon
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3603004/
https://www.ncbi.nlm.nih.gov/pubmed/23264558
http://dx.doi.org/10.1093/mp/sss160
work_keys_str_mv AT bertoliniedoardo addressingtheroleofmicrornasinreprogrammingleafgrowthduringdroughtstressinbrachypodiumdistachyon
AT verelstwim addressingtheroleofmicrornasinreprogrammingleafgrowthduringdroughtstressinbrachypodiumdistachyon
AT hornerdavidstephen addressingtheroleofmicrornasinreprogrammingleafgrowthduringdroughtstressinbrachypodiumdistachyon
AT gianfranceschiluca addressingtheroleofmicrornasinreprogrammingleafgrowthduringdroughtstressinbrachypodiumdistachyon
AT piccoloviviana addressingtheroleofmicrornasinreprogrammingleafgrowthduringdroughtstressinbrachypodiumdistachyon
AT inzedirk addressingtheroleofmicrornasinreprogrammingleafgrowthduringdroughtstressinbrachypodiumdistachyon
AT pemarioenrico addressingtheroleofmicrornasinreprogrammingleafgrowthduringdroughtstressinbrachypodiumdistachyon
AT micaerica addressingtheroleofmicrornasinreprogrammingleafgrowthduringdroughtstressinbrachypodiumdistachyon