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The RdDM Pathway Is Required for Basal Heat Tolerance in Arabidopsis

Heat stress affects epigenetic gene silencing in Arabidopsis. To test for a mechanistic involvement of epigenetic regulation in heat-stress responses, we analyzed the heat tolerance of mutants defective in DNA methylation, histone modifications, chromatin-remodeling, or siRNA-based silencing pathway...

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Detalles Bibliográficos
Autores principales: Popova, Olga V., Dinh, Huy Q., Aufsatz, Werner, Jonak, Claudia
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3603006/
https://www.ncbi.nlm.nih.gov/pubmed/23376771
http://dx.doi.org/10.1093/mp/sst023
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author Popova, Olga V.
Dinh, Huy Q.
Aufsatz, Werner
Jonak, Claudia
author_facet Popova, Olga V.
Dinh, Huy Q.
Aufsatz, Werner
Jonak, Claudia
author_sort Popova, Olga V.
collection PubMed
description Heat stress affects epigenetic gene silencing in Arabidopsis. To test for a mechanistic involvement of epigenetic regulation in heat-stress responses, we analyzed the heat tolerance of mutants defective in DNA methylation, histone modifications, chromatin-remodeling, or siRNA-based silencing pathways. Plants deficient in NRPD2, the common second-largest subunit of RNA polymerases IV and V, and in the Rpd3-type histone deacetylase HDA6 were hypersensitive to heat exposure. Microarray analysis demonstrated that NRPD2 and HDA6 have independent roles in transcriptional reprogramming in response to temperature stress. The misexpression of protein-coding genes in nrpd2 mutants recovering from heat correlated with defective epigenetic regulation of adjacent transposon remnants which involved the loss of control of heat-stress-induced read-through transcription. We provide evidence that the transcriptional response to temperature stress, at least partially, relies on the integrity of the RNA-dependent DNA methylation pathway.
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spelling pubmed-36030062013-03-20 The RdDM Pathway Is Required for Basal Heat Tolerance in Arabidopsis Popova, Olga V. Dinh, Huy Q. Aufsatz, Werner Jonak, Claudia Mol Plant Research Article Heat stress affects epigenetic gene silencing in Arabidopsis. To test for a mechanistic involvement of epigenetic regulation in heat-stress responses, we analyzed the heat tolerance of mutants defective in DNA methylation, histone modifications, chromatin-remodeling, or siRNA-based silencing pathways. Plants deficient in NRPD2, the common second-largest subunit of RNA polymerases IV and V, and in the Rpd3-type histone deacetylase HDA6 were hypersensitive to heat exposure. Microarray analysis demonstrated that NRPD2 and HDA6 have independent roles in transcriptional reprogramming in response to temperature stress. The misexpression of protein-coding genes in nrpd2 mutants recovering from heat correlated with defective epigenetic regulation of adjacent transposon remnants which involved the loss of control of heat-stress-induced read-through transcription. We provide evidence that the transcriptional response to temperature stress, at least partially, relies on the integrity of the RNA-dependent DNA methylation pathway. Oxford University Press 2013-03 2013-02-01 /pmc/articles/PMC3603006/ /pubmed/23376771 http://dx.doi.org/10.1093/mp/sst023 Text en © The Author 2013. Published by the Molecular Plant Shanghai Editorial Office in association with Oxford University Press on behalf of CSPB and IPPE, SIBS, CAS. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by-nc/3.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com.
spellingShingle Research Article
Popova, Olga V.
Dinh, Huy Q.
Aufsatz, Werner
Jonak, Claudia
The RdDM Pathway Is Required for Basal Heat Tolerance in Arabidopsis
title The RdDM Pathway Is Required for Basal Heat Tolerance in Arabidopsis
title_full The RdDM Pathway Is Required for Basal Heat Tolerance in Arabidopsis
title_fullStr The RdDM Pathway Is Required for Basal Heat Tolerance in Arabidopsis
title_full_unstemmed The RdDM Pathway Is Required for Basal Heat Tolerance in Arabidopsis
title_short The RdDM Pathway Is Required for Basal Heat Tolerance in Arabidopsis
title_sort rddm pathway is required for basal heat tolerance in arabidopsis
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3603006/
https://www.ncbi.nlm.nih.gov/pubmed/23376771
http://dx.doi.org/10.1093/mp/sst023
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