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Apoptosis through Bcl-2/Bax and Cleaved Caspase Up-Regulation in Melanoma Treated by Boron Neutron Capture Therapy

Boron neutron capture therapy (BNCT) is a binary treatment involving selective accumulation of boron carriers in a tumor followed by irradiation with a thermal or epithermal neutron beam. The neutron capture reaction with a boron-10 nucleus yields high linear energy transfer (LET) particles, alpha a...

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Autores principales: Faião-Flores, Fernanda, Coelho, Paulo Rogério Pinto, Toledo Arruda-Neto, João Dias, Maria-Engler, Silvya Stuchi, Tiago, Manoela, Capelozzi, Vera Luiza, Giorgi, Ricardo Rodrigues, Maria, Durvanei Augusto
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3603877/
https://www.ncbi.nlm.nih.gov/pubmed/23527236
http://dx.doi.org/10.1371/journal.pone.0059639
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author Faião-Flores, Fernanda
Coelho, Paulo Rogério Pinto
Toledo Arruda-Neto, João Dias
Maria-Engler, Silvya Stuchi
Tiago, Manoela
Capelozzi, Vera Luiza
Giorgi, Ricardo Rodrigues
Maria, Durvanei Augusto
author_facet Faião-Flores, Fernanda
Coelho, Paulo Rogério Pinto
Toledo Arruda-Neto, João Dias
Maria-Engler, Silvya Stuchi
Tiago, Manoela
Capelozzi, Vera Luiza
Giorgi, Ricardo Rodrigues
Maria, Durvanei Augusto
author_sort Faião-Flores, Fernanda
collection PubMed
description Boron neutron capture therapy (BNCT) is a binary treatment involving selective accumulation of boron carriers in a tumor followed by irradiation with a thermal or epithermal neutron beam. The neutron capture reaction with a boron-10 nucleus yields high linear energy transfer (LET) particles, alpha and (7)Li, with a range of 5 to 9 µm. These particles can only travel very short distances and release their damaging energy directly into the cells containing the boron compound. We aimed to evaluate proliferation, apoptosis and extracellular matrix (ECM) modifications of B16F10 melanoma and normal human melanocytes after BNCT. The amounts of soluble collagen and Hsp47, indicating collagen synthesis in the ECM, as well as the cellular markers of apoptosis, were investigated. BNCT decreased proliferation, altered the ECM by decreasing collagen synthesis and induced apoptosis by regulating Bcl-2/Bax in melanoma. Additionally, BNCT also increased the levels of TNF receptor and the cleaved caspases 3, 7, 8 and 9 in melanoma. These results suggest that multiple pathways related to cell death and cell cycle arrest are involved in the treatment of melanoma by BNCT.
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spelling pubmed-36038772013-03-22 Apoptosis through Bcl-2/Bax and Cleaved Caspase Up-Regulation in Melanoma Treated by Boron Neutron Capture Therapy Faião-Flores, Fernanda Coelho, Paulo Rogério Pinto Toledo Arruda-Neto, João Dias Maria-Engler, Silvya Stuchi Tiago, Manoela Capelozzi, Vera Luiza Giorgi, Ricardo Rodrigues Maria, Durvanei Augusto PLoS One Research Article Boron neutron capture therapy (BNCT) is a binary treatment involving selective accumulation of boron carriers in a tumor followed by irradiation with a thermal or epithermal neutron beam. The neutron capture reaction with a boron-10 nucleus yields high linear energy transfer (LET) particles, alpha and (7)Li, with a range of 5 to 9 µm. These particles can only travel very short distances and release their damaging energy directly into the cells containing the boron compound. We aimed to evaluate proliferation, apoptosis and extracellular matrix (ECM) modifications of B16F10 melanoma and normal human melanocytes after BNCT. The amounts of soluble collagen and Hsp47, indicating collagen synthesis in the ECM, as well as the cellular markers of apoptosis, were investigated. BNCT decreased proliferation, altered the ECM by decreasing collagen synthesis and induced apoptosis by regulating Bcl-2/Bax in melanoma. Additionally, BNCT also increased the levels of TNF receptor and the cleaved caspases 3, 7, 8 and 9 in melanoma. These results suggest that multiple pathways related to cell death and cell cycle arrest are involved in the treatment of melanoma by BNCT. Public Library of Science 2013-03-20 /pmc/articles/PMC3603877/ /pubmed/23527236 http://dx.doi.org/10.1371/journal.pone.0059639 Text en © 2013 Faião-Flores et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Faião-Flores, Fernanda
Coelho, Paulo Rogério Pinto
Toledo Arruda-Neto, João Dias
Maria-Engler, Silvya Stuchi
Tiago, Manoela
Capelozzi, Vera Luiza
Giorgi, Ricardo Rodrigues
Maria, Durvanei Augusto
Apoptosis through Bcl-2/Bax and Cleaved Caspase Up-Regulation in Melanoma Treated by Boron Neutron Capture Therapy
title Apoptosis through Bcl-2/Bax and Cleaved Caspase Up-Regulation in Melanoma Treated by Boron Neutron Capture Therapy
title_full Apoptosis through Bcl-2/Bax and Cleaved Caspase Up-Regulation in Melanoma Treated by Boron Neutron Capture Therapy
title_fullStr Apoptosis through Bcl-2/Bax and Cleaved Caspase Up-Regulation in Melanoma Treated by Boron Neutron Capture Therapy
title_full_unstemmed Apoptosis through Bcl-2/Bax and Cleaved Caspase Up-Regulation in Melanoma Treated by Boron Neutron Capture Therapy
title_short Apoptosis through Bcl-2/Bax and Cleaved Caspase Up-Regulation in Melanoma Treated by Boron Neutron Capture Therapy
title_sort apoptosis through bcl-2/bax and cleaved caspase up-regulation in melanoma treated by boron neutron capture therapy
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3603877/
https://www.ncbi.nlm.nih.gov/pubmed/23527236
http://dx.doi.org/10.1371/journal.pone.0059639
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