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Oral Ingestion of Transgenic RIDL Ae. aegypti Larvae Has No Negative Effect on Two Predator Toxorhynchites Species

Dengue is the most important mosquito-borne viral disease. No specific treatment or vaccine is currently available; traditional vector control methods can rarely achieve adequate control. Recently, the RIDL (Release of Insect carrying Dominant Lethality) approach has been developed, based on the ste...

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Autores principales: Nordin, Oreenaiza, Donald, Wesley, Ming, Wong Hong, Ney, Teoh Guat, Mohamed, Khairul Asuad, Halim, Nor Azlina Abdul, Winskill, Peter, Hadi, Azahari Abdul, Muhammad, Zulkamal Safi'in, Lacroix, Renaud, Scaife, Sarah, McKemey, Andrew Robert, Beech, Camilla, Shahnaz, Murad, Alphey, Luke, Nimmo, Derric David, Nazni, Wasi Ahmed, Lee, Han Lim
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3604150/
https://www.ncbi.nlm.nih.gov/pubmed/23527029
http://dx.doi.org/10.1371/journal.pone.0058805
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author Nordin, Oreenaiza
Donald, Wesley
Ming, Wong Hong
Ney, Teoh Guat
Mohamed, Khairul Asuad
Halim, Nor Azlina Abdul
Winskill, Peter
Hadi, Azahari Abdul
Muhammad, Zulkamal Safi'in
Lacroix, Renaud
Scaife, Sarah
McKemey, Andrew Robert
Beech, Camilla
Shahnaz, Murad
Alphey, Luke
Nimmo, Derric David
Nazni, Wasi Ahmed
Lee, Han Lim
author_facet Nordin, Oreenaiza
Donald, Wesley
Ming, Wong Hong
Ney, Teoh Guat
Mohamed, Khairul Asuad
Halim, Nor Azlina Abdul
Winskill, Peter
Hadi, Azahari Abdul
Muhammad, Zulkamal Safi'in
Lacroix, Renaud
Scaife, Sarah
McKemey, Andrew Robert
Beech, Camilla
Shahnaz, Murad
Alphey, Luke
Nimmo, Derric David
Nazni, Wasi Ahmed
Lee, Han Lim
author_sort Nordin, Oreenaiza
collection PubMed
description Dengue is the most important mosquito-borne viral disease. No specific treatment or vaccine is currently available; traditional vector control methods can rarely achieve adequate control. Recently, the RIDL (Release of Insect carrying Dominant Lethality) approach has been developed, based on the sterile insect technique, in which genetically engineered ‘sterile’ homozygous RIDL male insects are released to mate wild females; the offspring inherit a copy of the RIDL construct and die. A RIDL strain of the dengue mosquito, Aedes aegypti, OX513A, expresses a fluorescent marker gene for identification (DsRed2) and a protein (tTAV) that causes the offspring to die. We examined whether these proteins could adversely affect predators that may feed on the insect. Aedes aegypti is a peri-domestic mosquito that typically breeds in small, rain-water-filled containers and has no specific predators. Toxorhynchites larvae feed on small aquatic organisms and are easily reared in the laboratory where they can be fed exclusively on mosquito larvae. To evaluate the effect of a predator feeding on a diet of RIDL insects, OX513A Ae. aegypti larvae were fed to two different species of Toxorhynchites (Tx. splendens and Tx. amboinensis) and effects on life table parameters of all life stages were compared to being fed on wild type larvae. No significant negative effect was observed on any life table parameter studied; this outcome and the benign nature of the expressed proteins (tTAV and DsRed2) indicate that Ae. aegypti OX513A RIDL strain is unlikely to have any adverse effects on predators in the environment.
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spelling pubmed-36041502013-03-22 Oral Ingestion of Transgenic RIDL Ae. aegypti Larvae Has No Negative Effect on Two Predator Toxorhynchites Species Nordin, Oreenaiza Donald, Wesley Ming, Wong Hong Ney, Teoh Guat Mohamed, Khairul Asuad Halim, Nor Azlina Abdul Winskill, Peter Hadi, Azahari Abdul Muhammad, Zulkamal Safi'in Lacroix, Renaud Scaife, Sarah McKemey, Andrew Robert Beech, Camilla Shahnaz, Murad Alphey, Luke Nimmo, Derric David Nazni, Wasi Ahmed Lee, Han Lim PLoS One Research Article Dengue is the most important mosquito-borne viral disease. No specific treatment or vaccine is currently available; traditional vector control methods can rarely achieve adequate control. Recently, the RIDL (Release of Insect carrying Dominant Lethality) approach has been developed, based on the sterile insect technique, in which genetically engineered ‘sterile’ homozygous RIDL male insects are released to mate wild females; the offspring inherit a copy of the RIDL construct and die. A RIDL strain of the dengue mosquito, Aedes aegypti, OX513A, expresses a fluorescent marker gene for identification (DsRed2) and a protein (tTAV) that causes the offspring to die. We examined whether these proteins could adversely affect predators that may feed on the insect. Aedes aegypti is a peri-domestic mosquito that typically breeds in small, rain-water-filled containers and has no specific predators. Toxorhynchites larvae feed on small aquatic organisms and are easily reared in the laboratory where they can be fed exclusively on mosquito larvae. To evaluate the effect of a predator feeding on a diet of RIDL insects, OX513A Ae. aegypti larvae were fed to two different species of Toxorhynchites (Tx. splendens and Tx. amboinensis) and effects on life table parameters of all life stages were compared to being fed on wild type larvae. No significant negative effect was observed on any life table parameter studied; this outcome and the benign nature of the expressed proteins (tTAV and DsRed2) indicate that Ae. aegypti OX513A RIDL strain is unlikely to have any adverse effects on predators in the environment. Public Library of Science 2013-03-20 /pmc/articles/PMC3604150/ /pubmed/23527029 http://dx.doi.org/10.1371/journal.pone.0058805 Text en © 2013 Nordin et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Nordin, Oreenaiza
Donald, Wesley
Ming, Wong Hong
Ney, Teoh Guat
Mohamed, Khairul Asuad
Halim, Nor Azlina Abdul
Winskill, Peter
Hadi, Azahari Abdul
Muhammad, Zulkamal Safi'in
Lacroix, Renaud
Scaife, Sarah
McKemey, Andrew Robert
Beech, Camilla
Shahnaz, Murad
Alphey, Luke
Nimmo, Derric David
Nazni, Wasi Ahmed
Lee, Han Lim
Oral Ingestion of Transgenic RIDL Ae. aegypti Larvae Has No Negative Effect on Two Predator Toxorhynchites Species
title Oral Ingestion of Transgenic RIDL Ae. aegypti Larvae Has No Negative Effect on Two Predator Toxorhynchites Species
title_full Oral Ingestion of Transgenic RIDL Ae. aegypti Larvae Has No Negative Effect on Two Predator Toxorhynchites Species
title_fullStr Oral Ingestion of Transgenic RIDL Ae. aegypti Larvae Has No Negative Effect on Two Predator Toxorhynchites Species
title_full_unstemmed Oral Ingestion of Transgenic RIDL Ae. aegypti Larvae Has No Negative Effect on Two Predator Toxorhynchites Species
title_short Oral Ingestion of Transgenic RIDL Ae. aegypti Larvae Has No Negative Effect on Two Predator Toxorhynchites Species
title_sort oral ingestion of transgenic ridl ae. aegypti larvae has no negative effect on two predator toxorhynchites species
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3604150/
https://www.ncbi.nlm.nih.gov/pubmed/23527029
http://dx.doi.org/10.1371/journal.pone.0058805
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