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Direct-write, highly aligned chitosan-poly(ethylene oxide) nanofiber patterns for cell morphology and spreading control

Near-field electrospinning has been demonstrated to be able to achieve direct-write and highly aligned chitosan nanofibers (CNF) with prescribed positioning density. Cell spreading in preferential direction could be observed on parallel-aligned nanofibers, and the CNF patterns were capable of guidin...

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Detalles Bibliográficos
Autores principales: Fuh, Yiin Kuen, Chen, Sheng Zhan, He, Zhe Yu
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3605135/
https://www.ncbi.nlm.nih.gov/pubmed/23433121
http://dx.doi.org/10.1186/1556-276X-8-97
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author Fuh, Yiin Kuen
Chen, Sheng Zhan
He, Zhe Yu
author_facet Fuh, Yiin Kuen
Chen, Sheng Zhan
He, Zhe Yu
author_sort Fuh, Yiin Kuen
collection PubMed
description Near-field electrospinning has been demonstrated to be able to achieve direct-write and highly aligned chitosan nanofibers (CNF) with prescribed positioning density. Cell spreading in preferential direction could be observed on parallel-aligned nanofibers, and the CNF patterns were capable of guiding cell extension when the distances between them are 20 and 100 μm, respectively. Alignment of the cells was characterized according to their elongation and orientation using the fast Fourier transform data and binary image analysis. Parallel CNF indicates that the alignment values sequentially increased as a function of positioning density such that incrementally more aligned cells were closely related to the increasing CNF positioning density. These maskless, low-cost, and direct-write patterns can be facily fabricated and will be a promising tool to study cell-based research such as cell adhesion, spreading, and tissue architecture.
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spelling pubmed-36051352013-03-25 Direct-write, highly aligned chitosan-poly(ethylene oxide) nanofiber patterns for cell morphology and spreading control Fuh, Yiin Kuen Chen, Sheng Zhan He, Zhe Yu Nanoscale Res Lett Nano Express Near-field electrospinning has been demonstrated to be able to achieve direct-write and highly aligned chitosan nanofibers (CNF) with prescribed positioning density. Cell spreading in preferential direction could be observed on parallel-aligned nanofibers, and the CNF patterns were capable of guiding cell extension when the distances between them are 20 and 100 μm, respectively. Alignment of the cells was characterized according to their elongation and orientation using the fast Fourier transform data and binary image analysis. Parallel CNF indicates that the alignment values sequentially increased as a function of positioning density such that incrementally more aligned cells were closely related to the increasing CNF positioning density. These maskless, low-cost, and direct-write patterns can be facily fabricated and will be a promising tool to study cell-based research such as cell adhesion, spreading, and tissue architecture. Springer 2013-02-22 /pmc/articles/PMC3605135/ /pubmed/23433121 http://dx.doi.org/10.1186/1556-276X-8-97 Text en Copyright ©2013 Fuh et al; licensee Springer. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Nano Express
Fuh, Yiin Kuen
Chen, Sheng Zhan
He, Zhe Yu
Direct-write, highly aligned chitosan-poly(ethylene oxide) nanofiber patterns for cell morphology and spreading control
title Direct-write, highly aligned chitosan-poly(ethylene oxide) nanofiber patterns for cell morphology and spreading control
title_full Direct-write, highly aligned chitosan-poly(ethylene oxide) nanofiber patterns for cell morphology and spreading control
title_fullStr Direct-write, highly aligned chitosan-poly(ethylene oxide) nanofiber patterns for cell morphology and spreading control
title_full_unstemmed Direct-write, highly aligned chitosan-poly(ethylene oxide) nanofiber patterns for cell morphology and spreading control
title_short Direct-write, highly aligned chitosan-poly(ethylene oxide) nanofiber patterns for cell morphology and spreading control
title_sort direct-write, highly aligned chitosan-poly(ethylene oxide) nanofiber patterns for cell morphology and spreading control
topic Nano Express
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3605135/
https://www.ncbi.nlm.nih.gov/pubmed/23433121
http://dx.doi.org/10.1186/1556-276X-8-97
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