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MicroRNA-613 represses lipogenesis in HepG2 cells by downregulating LXRα

BACKGROUND: MicroRNAs (miRNAs) emerge as new important regulators of lipid homeostasis by regulating corresponding genes. MiR-613 is a newly discovered microRNA, of which the biological function is unknown. A recent report has shown that miR-613 downregulates liver X receptor α (LXRα), a ligand-acti...

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Autores principales: Zhong, Dan, Zhang, Yan, Zeng, Yi-jun, Gao, Min, Wu, Geng-ze, Hu, Chang-jiang, Huang, Gang, He, Feng-tian
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3605323/
https://www.ncbi.nlm.nih.gov/pubmed/23496987
http://dx.doi.org/10.1186/1476-511X-12-32
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author Zhong, Dan
Zhang, Yan
Zeng, Yi-jun
Gao, Min
Wu, Geng-ze
Hu, Chang-jiang
Huang, Gang
He, Feng-tian
author_facet Zhong, Dan
Zhang, Yan
Zeng, Yi-jun
Gao, Min
Wu, Geng-ze
Hu, Chang-jiang
Huang, Gang
He, Feng-tian
author_sort Zhong, Dan
collection PubMed
description BACKGROUND: MicroRNAs (miRNAs) emerge as new important regulators of lipid homeostasis by regulating corresponding genes. MiR-613 is a newly discovered microRNA, of which the biological function is unknown. A recent report has shown that miR-613 downregulates liver X receptor α (LXRα), a ligand-activated nuclear receptor playing an important role in the regulation of lipid metabolism. The purpose of this study is to explore the effect and the molecular basis of miR-613 on lipogenesis in HepG2 cells. METHODS: HepG2 cells were transiently transfected with miR-613 mimic or control microRNA. Real time PCR, Western blot, Luciferase reporter assay and Oil Red O staining were employed to examine the expression of LXRα and its target genes involved in lipogenesis, binding site for miR-613 in 3(′)-untranslated region (3(′)-UTR) of LXRα mRNA and lipid droplet accumulation in the cells. RESULTS: MiR-613 dramatically suppressed the expression of LXRα and its target genes including sterol-regulatory element binding protein 1c (SREBP-1c), fatty acid synthase (FAS), carbohydrate responsive element-binding protein (ChREBP) and acetyl-CoA carboxylase (ACC). Reporter assay showed that miR-613 directly bound to 3(′)-UTR of LXRα mRNA. Moreover, miR-613 significantly repressed LXRα-induced lipid droplet accumulation in HepG2 cells. Ectopic expression of LXRα without 3(′)-UTR markedly attenuated the miR-613-mediated downregulation of LXRα’s target genes and LXRα-induced lipid droplet accumulation. CONCLUSIONS: MiR-613 suppresses lipogenesis by directly targeting LXRα in HepG2 cells, suggesting that miR-613 may serve as a novel target for regulating lipid homeostasis.
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spelling pubmed-36053232013-03-23 MicroRNA-613 represses lipogenesis in HepG2 cells by downregulating LXRα Zhong, Dan Zhang, Yan Zeng, Yi-jun Gao, Min Wu, Geng-ze Hu, Chang-jiang Huang, Gang He, Feng-tian Lipids Health Dis Research BACKGROUND: MicroRNAs (miRNAs) emerge as new important regulators of lipid homeostasis by regulating corresponding genes. MiR-613 is a newly discovered microRNA, of which the biological function is unknown. A recent report has shown that miR-613 downregulates liver X receptor α (LXRα), a ligand-activated nuclear receptor playing an important role in the regulation of lipid metabolism. The purpose of this study is to explore the effect and the molecular basis of miR-613 on lipogenesis in HepG2 cells. METHODS: HepG2 cells were transiently transfected with miR-613 mimic or control microRNA. Real time PCR, Western blot, Luciferase reporter assay and Oil Red O staining were employed to examine the expression of LXRα and its target genes involved in lipogenesis, binding site for miR-613 in 3(′)-untranslated region (3(′)-UTR) of LXRα mRNA and lipid droplet accumulation in the cells. RESULTS: MiR-613 dramatically suppressed the expression of LXRα and its target genes including sterol-regulatory element binding protein 1c (SREBP-1c), fatty acid synthase (FAS), carbohydrate responsive element-binding protein (ChREBP) and acetyl-CoA carboxylase (ACC). Reporter assay showed that miR-613 directly bound to 3(′)-UTR of LXRα mRNA. Moreover, miR-613 significantly repressed LXRα-induced lipid droplet accumulation in HepG2 cells. Ectopic expression of LXRα without 3(′)-UTR markedly attenuated the miR-613-mediated downregulation of LXRα’s target genes and LXRα-induced lipid droplet accumulation. CONCLUSIONS: MiR-613 suppresses lipogenesis by directly targeting LXRα in HepG2 cells, suggesting that miR-613 may serve as a novel target for regulating lipid homeostasis. BioMed Central 2013-03-08 /pmc/articles/PMC3605323/ /pubmed/23496987 http://dx.doi.org/10.1186/1476-511X-12-32 Text en Copyright ©2013 Zhong et al; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research
Zhong, Dan
Zhang, Yan
Zeng, Yi-jun
Gao, Min
Wu, Geng-ze
Hu, Chang-jiang
Huang, Gang
He, Feng-tian
MicroRNA-613 represses lipogenesis in HepG2 cells by downregulating LXRα
title MicroRNA-613 represses lipogenesis in HepG2 cells by downregulating LXRα
title_full MicroRNA-613 represses lipogenesis in HepG2 cells by downregulating LXRα
title_fullStr MicroRNA-613 represses lipogenesis in HepG2 cells by downregulating LXRα
title_full_unstemmed MicroRNA-613 represses lipogenesis in HepG2 cells by downregulating LXRα
title_short MicroRNA-613 represses lipogenesis in HepG2 cells by downregulating LXRα
title_sort microrna-613 represses lipogenesis in hepg2 cells by downregulating lxrα
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3605323/
https://www.ncbi.nlm.nih.gov/pubmed/23496987
http://dx.doi.org/10.1186/1476-511X-12-32
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