Cargando…

Improved assay to detect Plasmodium falciparum using an uninterrupted, semi-nested PCR and quantitative lateral flow analysis

BACKGROUND: A rapid, non-invasive, and inexpensive point-of-care (POC) diagnostic for malaria followed by therapeutic intervention would improve the ability to control infection in endemic areas. METHODS: A semi-nested PCR amplification protocol is described for quantitative detection of Plasmodium...

Descripción completa

Detalles Bibliográficos
Autores principales:	Ongagna-Yhombi, Serge Y, Corstjens, Paul, Geva, Eran, Abrams, William R, Barber, Cheryl A, Malamud, Daniel, Mharakurwa, Sungano
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	BioMed Central 2013
Materias:	Methodology
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3605351/ https://www.ncbi.nlm.nih.gov/pubmed/23433252 http://dx.doi.org/10.1186/1475-2875-12-74

Ejemplares similares

Rapid Point-of-Care Isothermal Amplification Assay for the Detection of Malaria without Nucleic Acid Purification
por: Modak, Sayli S., et al.
Publicado: (2016)

PCR detection of Plasmodium falciparum in human urine and saliva samples
por: Mharakurwa, Sungano, et al.
Publicado: (2006)

Pre-amplification methods for tracking low-grade Plasmodium falciparum populations during scaled-up interventions in Southern Zambia
por: Mharakurwa, Sungano, et al.
Publicado: (2014)

Simultaneous detection of Plasmodium vivax and Plasmodium falciparum gametocytes in clinical isolates by multiplex-nested RT-PCR
por: Kuamsab, Napaporn, et al.
Publicado: (2012)

A nested real-time PCR assay for the quantification of Plasmodium falciparum DNA extracted from dried blood spots
por: Tran, Tuan M, et al.
Publicado: (2014)

A Rapid, Self-confirming Assay for HIV: Simultaneous Detection of Anti-HIV Antibodies and Viral RNA
por: Chen, Zongyuan, et al.
Publicado: (2016)

Semi-high-throughput detection of Plasmodium falciparum and Plasmodium vivax oocysts in mosquitoes using bead-beating followed by circumsporozoite ELISA and quantitative PCR
por: Graumans, Wouter, et al.
Publicado: (2017)

Selection for chloroquine-sensitive Plasmodium falciparum by wild Anopheles arabiensis in Southern Zambia
por: Mharakurwa, Sungano, et al.
Publicado: (2013)

Rural health centres, communities and malaria case detection in Zambia using mobile telephones: a means to detect potential reservoirs of infection in unstable transmission conditions
por: Kamanga, Aniset, et al.
Publicado: (2010)

Escalating Plasmodium falciparum antifolate drug resistance mutations in Macha, rural Zambia
por: Mkulama, Mtawa AP, et al.
Publicado: (2008)

Distinction of Plasmodium falciparum recrudescence and re-infection by MSP2 genotyping: A caution about unstandardized classification criteria
por: Rouse, Petrica, et al.
Publicado: (2008)

Streamlined, PCR-based testing for pfhrp2- and pfhrp3-negative Plasmodium falciparum
por: Parr, Jonathan B., et al.
Publicado: (2018)

High prevalence of dhfr and dhps molecular markers in Plasmodium falciparum in pregnant women of Nchelenge district, Northern Zambia
por: Siame, Mwiche NP, et al.
Publicado: (2015)

A highly sensitive, PCR-based method for the detection of Plasmodium falciparum clones in microtiter plates
por: Maher, Steven P, et al.
Publicado: (2008)

Measurement of gene amplifications related to drug resistance in Plasmodium falciparum using droplet digital PCR
por: Srisutham, Suttipat, et al.
Publicado: (2021)

A direct comparison of real time PCR on plasma and blood to detect Plasmodium falciparum infection in children
por: Lamikanra, Abigail A, et al.
Publicado: (2012)

A new method for detection of pfmdr1 mutations in Plasmodium falciparum DNA using real-time PCR
por: Purfield, Anne, et al.
Publicado: (2004)

In vivo transcriptional profiling of Plasmodium falciparum
por: Daily, Johanna P, et al.
Publicado: (2004)

Laser-induced inactivation of Plasmodium falciparum
por: LeBlanc, Danielle, et al.
Publicado: (2012)

New synchronization method for Plasmodium falciparum
por: Ranford-Cartwright, Lisa C, et al.
Publicado: (2010)

Comparison of Plasmodium falciparum transfection methods
por: Skinner-Adams, Tina S, et al.
Publicado: (2003)

Validation of oral fluid samples to monitor serological changes to Plasmodium falciparum: An observational study in southern Zambia
por: Chidi, Alexis P, et al.
Publicado: (2011)

Sulfadoxine-pyrimethamine parasitological efficacy against Plasmodium falciparum among pregnant women and molecular markers of resistance in Zambia: an observational cohort study
por: Chaponda, Enesia Banda, et al.
Publicado: (2021)

A nuclear targeting system in Plasmodium falciparum
por: Wittayacom, Kanjana, et al.
Publicado: (2010)

Automated counting for Plasmodium falciparum cytoadherence experiments
por: Paton, Douglas, et al.
Publicado: (2011)

Comparison of three molecular methods for the detection and speciation of Plasmodium vivax and Plasmodium falciparum
por: Boonma, Prapaporn, et al.
Publicado: (2007)

Evaluation of a real-time quantitative PCR to measure the wild Plasmodium falciparum infectivity rate in salivary glands of Anopheles gambiae
por: Marie, Alexandra, et al.
Publicado: (2013)

Comparison of molecular quantification of Plasmodium falciparum gametocytes by Pfs25 qRT-PCR and QT-NASBA in relation to mosquito infectivity
por: Pett, Helmi, et al.
Publicado: (2016)

A real-time, quantitative PCR method using hydrolysis probes for the monitoring of Plasmodium falciparum load in experimentally infected human volunteers
por: Rockett, Rebecca J, et al.
Publicado: (2011)

Rapid whole genome optical mapping of Plasmodium falciparum
por: Riley, Matthew C, et al.
Publicado: (2011)

Temporal and spatial patterns of serologic responses to Plasmodium falciparum antigens in a region of declining malaria transmission in southern Zambia
por: Kobayashi, Tamaki, et al.
Publicado: (2012)

Real-time PCR/MCA assay using fluorescence resonance energy transfer for the genotyping of resistance related DHPS-540 mutations in Plasmodium falciparum
por: Mens, Petra F, et al.
Publicado: (2008)

Real-time quantitative PCR with SYBR Green I detection for estimating copy numbers of nine drug resistance candidate genes in Plasmodium falciparum
por: Ferreira, Isabel D, et al.
Publicado: (2006)

The relationship between the haemoglobin concentration and the haematocrit in Plasmodium falciparum malaria
por: Lee, Sue J, et al.
Publicado: (2008)

Development of fluorescent Plasmodium falciparum for in vitro growth inhibition assays
por: Wilson, Danny W, et al.
Publicado: (2010)

An improved method for the in vitro differentiation of Plasmodium falciparum gametocytes into ookinetes
por: Ghosh, Anil K, et al.
Publicado: (2010)

Evaluation of Plasmodium falciparum gametocyte detection in different patient material
por: Kast, Katharina, et al.
Publicado: (2013)

Re-evaluation of microscopy confirmed Plasmodium falciparum and Plasmodium vivax malaria by nested PCR detection in southern Ethiopia
por: Mekonnen, Seleshi Kebede, et al.
Publicado: (2014)

A regulatable transgene expression system for cultured Plasmodium falciparum parasites
por: Epp, Christian, et al.
Publicado: (2008)

Measures of clinical malaria in field trials of interventions against Plasmodium falciparum
por: Smith, Thomas A
Publicado: (2007)

Cannot write session to /tmp/vufind_sessions/sess_24let47otrhf91mn6m7khkkvs8