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Functional effect of mir-27b on myostatin expression: a relationship in piedmontese cattle with double-muscled phenotype
BACKGROUND: In Piedmontese cattle the double-muscled phenotype is an inherited condition associated to a point mutation in the myostatin (MSTN) gene. The Piedmontese MSTN missense mutation G938A is translated to C313Y myostatin protein. This mutation alters MSTN function as a negative regulator of m...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2013
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3605361/ https://www.ncbi.nlm.nih.gov/pubmed/23510267 http://dx.doi.org/10.1186/1471-2164-14-194 |
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author | Miretti, Silvia Martignani, Eugenio Accornero, Paolo Baratta, Mario |
author_facet | Miretti, Silvia Martignani, Eugenio Accornero, Paolo Baratta, Mario |
author_sort | Miretti, Silvia |
collection | PubMed |
description | BACKGROUND: In Piedmontese cattle the double-muscled phenotype is an inherited condition associated to a point mutation in the myostatin (MSTN) gene. The Piedmontese MSTN missense mutation G938A is translated to C313Y myostatin protein. This mutation alters MSTN function as a negative regulator of muscle growth, thereby inducing muscle hypertrophy. MiRNAs could play a role in skeletal muscle hypertrophy modulation by down-regulating gene expression. RESULTS: After identifying a 3(′)-UTR consensus sequence of several negative and positive modulator genes involved in the skeletal muscle hypertrophy pathway, such as IGF1, IGF1R, PPP3CA, NFATc1, MEF2C, GSK3b, TEAD1 and MSTN, we screened miRNAs matching to it. This analysis led to the identification of miR-27b, miR-132, miR-186 and miR-199b-5p as possible candidates. We collected samples of longissimus thoracis from twenty Piedmontese and twenty Friesian male bovines. In Piedmontese group miR-27b was up-regulated 7.4-fold (p < 0.05). Further, we report that the level of MSTN mRNA was about 5-fold lower in Piedmontese cattle vs Friesian cattle (p < 0.0001) and that less mature MSTN protein was detected in the Piedmontese one (p < 0.0001). Cotransfection of miR-27b and psi-check2 vector with the luciferase reporter gene linked to the bovine wild-type 3(′)-UTR of MSTN strongly inhibited the luciferase activity (79%, p < 0.0001). CONCLUSIONS: These data demonstrate that bovine MSTN is a specific target of miR-27b and that miRNAs contribute to explain additive phenotypic hypertrophy in Piedmontese cattle selected for the MSTN gene mutation, possibly outlining a more precise genetic signature able to elucidate differences in muscle conformation. |
format | Online Article Text |
id | pubmed-3605361 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2013 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-36053612013-03-23 Functional effect of mir-27b on myostatin expression: a relationship in piedmontese cattle with double-muscled phenotype Miretti, Silvia Martignani, Eugenio Accornero, Paolo Baratta, Mario BMC Genomics Research Article BACKGROUND: In Piedmontese cattle the double-muscled phenotype is an inherited condition associated to a point mutation in the myostatin (MSTN) gene. The Piedmontese MSTN missense mutation G938A is translated to C313Y myostatin protein. This mutation alters MSTN function as a negative regulator of muscle growth, thereby inducing muscle hypertrophy. MiRNAs could play a role in skeletal muscle hypertrophy modulation by down-regulating gene expression. RESULTS: After identifying a 3(′)-UTR consensus sequence of several negative and positive modulator genes involved in the skeletal muscle hypertrophy pathway, such as IGF1, IGF1R, PPP3CA, NFATc1, MEF2C, GSK3b, TEAD1 and MSTN, we screened miRNAs matching to it. This analysis led to the identification of miR-27b, miR-132, miR-186 and miR-199b-5p as possible candidates. We collected samples of longissimus thoracis from twenty Piedmontese and twenty Friesian male bovines. In Piedmontese group miR-27b was up-regulated 7.4-fold (p < 0.05). Further, we report that the level of MSTN mRNA was about 5-fold lower in Piedmontese cattle vs Friesian cattle (p < 0.0001) and that less mature MSTN protein was detected in the Piedmontese one (p < 0.0001). Cotransfection of miR-27b and psi-check2 vector with the luciferase reporter gene linked to the bovine wild-type 3(′)-UTR of MSTN strongly inhibited the luciferase activity (79%, p < 0.0001). CONCLUSIONS: These data demonstrate that bovine MSTN is a specific target of miR-27b and that miRNAs contribute to explain additive phenotypic hypertrophy in Piedmontese cattle selected for the MSTN gene mutation, possibly outlining a more precise genetic signature able to elucidate differences in muscle conformation. BioMed Central 2013-03-19 /pmc/articles/PMC3605361/ /pubmed/23510267 http://dx.doi.org/10.1186/1471-2164-14-194 Text en Copyright ©2013 Miretti et al.; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Miretti, Silvia Martignani, Eugenio Accornero, Paolo Baratta, Mario Functional effect of mir-27b on myostatin expression: a relationship in piedmontese cattle with double-muscled phenotype |
title | Functional effect of mir-27b on myostatin expression: a relationship in piedmontese cattle with double-muscled phenotype |
title_full | Functional effect of mir-27b on myostatin expression: a relationship in piedmontese cattle with double-muscled phenotype |
title_fullStr | Functional effect of mir-27b on myostatin expression: a relationship in piedmontese cattle with double-muscled phenotype |
title_full_unstemmed | Functional effect of mir-27b on myostatin expression: a relationship in piedmontese cattle with double-muscled phenotype |
title_short | Functional effect of mir-27b on myostatin expression: a relationship in piedmontese cattle with double-muscled phenotype |
title_sort | functional effect of mir-27b on myostatin expression: a relationship in piedmontese cattle with double-muscled phenotype |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3605361/ https://www.ncbi.nlm.nih.gov/pubmed/23510267 http://dx.doi.org/10.1186/1471-2164-14-194 |
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