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Quantitative optical trapping on single cellular organelles in cell extract
We develop optical trapping methodology to precisely measure the force generated by motor-proteins on single organelles of unknown size in cell extract. Native motor-complexes can now be interrogated functionally, overcoming limitations of assays with purified motors coated on artificial beads. Forc...
| Autores principales: | , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
2012
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3605797/ https://www.ncbi.nlm.nih.gov/pubmed/23241632 http://dx.doi.org/10.1038/nmeth.2287 |