RNAi-mediated abrogation of trehalase expression does not affect trehalase activity in sugarcane

To engineer trehalose metabolism in sugarcane (Saccharum spp. hybrids) two transgenes were introduced to the genome: trehalose-6-phosphate synthase- phosphatase (TPSP), to increase trehalose biosynthesis and an RNAi transgene specific for trehalase, to abrogate trehalose catabolism. In RNAi-expressi...

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Detalles Bibliográficos
Autores principales: O’Neill, Brian P, Purnell, Matthew P, Nielsen, Lars K, Brumbley, Stevens M
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer International Publishing AG 2012
Materias:
Research
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3606522/
https://www.ncbi.nlm.nih.gov/pubmed/23539210
http://dx.doi.org/10.1186/2193-1801-1-74
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author O’Neill, Brian P
Purnell, Matthew P
Nielsen, Lars K
Brumbley, Stevens M
author_facet O’Neill, Brian P
Purnell, Matthew P
Nielsen, Lars K
Brumbley, Stevens M
author_sort O’Neill, Brian P
collection PubMed
description To engineer trehalose metabolism in sugarcane (Saccharum spp. hybrids) two transgenes were introduced to the genome: trehalose-6-phosphate synthase- phosphatase (TPSP), to increase trehalose biosynthesis and an RNAi transgene specific for trehalase, to abrogate trehalose catabolism. In RNAi-expressing lines trehalase expression was abrogated in many plants however no decrease in trehalase activity was observed. In TPSP lines trehalase activity was significantly higher. No events of co-integration of TPSP and RNAi transgenes were observed. We suggest trehalase activity is essential to mitigate embryonic lethal effects of trehalose metabolism and discuss the implications for engineering trehalose metabolism.
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spelling pubmed-36065222013-03-25 RNAi-mediated abrogation of trehalase expression does not affect trehalase activity in sugarcane O’Neill, Brian P Purnell, Matthew P Nielsen, Lars K Brumbley, Stevens M Springerplus Research To engineer trehalose metabolism in sugarcane (Saccharum spp. hybrids) two transgenes were introduced to the genome: trehalose-6-phosphate synthase- phosphatase (TPSP), to increase trehalose biosynthesis and an RNAi transgene specific for trehalase, to abrogate trehalose catabolism. In RNAi-expressing lines trehalase expression was abrogated in many plants however no decrease in trehalase activity was observed. In TPSP lines trehalase activity was significantly higher. No events of co-integration of TPSP and RNAi transgenes were observed. We suggest trehalase activity is essential to mitigate embryonic lethal effects of trehalose metabolism and discuss the implications for engineering trehalose metabolism. Springer International Publishing AG 2012-12-21 /pmc/articles/PMC3606522/ /pubmed/23539210 http://dx.doi.org/10.1186/2193-1801-1-74 Text en © O'Neill et al.; licensee Springer. 2012 This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research
O’Neill, Brian P
Purnell, Matthew P
Nielsen, Lars K
Brumbley, Stevens M
RNAi-mediated abrogation of trehalase expression does not affect trehalase activity in sugarcane
title RNAi-mediated abrogation of trehalase expression does not affect trehalase activity in sugarcane
title_full RNAi-mediated abrogation of trehalase expression does not affect trehalase activity in sugarcane
title_fullStr RNAi-mediated abrogation of trehalase expression does not affect trehalase activity in sugarcane
title_full_unstemmed RNAi-mediated abrogation of trehalase expression does not affect trehalase activity in sugarcane
title_short RNAi-mediated abrogation of trehalase expression does not affect trehalase activity in sugarcane
title_sort rnai-mediated abrogation of trehalase expression does not affect trehalase activity in sugarcane
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3606522/
https://www.ncbi.nlm.nih.gov/pubmed/23539210
http://dx.doi.org/10.1186/2193-1801-1-74
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