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Identification of differentially expressed genes from Trichoderma harzianum during growth on cell wall of Fusarium solani as a tool for biotechnological application

BACKGROUND: The species of T. harzianum are well known for their biocontrol activity against many plant pathogens. However, there is a lack of studies concerning its use as a biological control agent against F. solani, a pathogen involved in several crop diseases. In this study, we have used subtrac...

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Autores principales: Vieira, Pabline Marinho, Coelho, Alexandre Siqueira Guedes, Steindorff, Andrei Stecca, de Siqueira, Saulo José Linhares, Silva, Roberto do Nascimento, Ulhoa, Cirano José
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3606605/
https://www.ncbi.nlm.nih.gov/pubmed/23497274
http://dx.doi.org/10.1186/1471-2164-14-177
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author Vieira, Pabline Marinho
Coelho, Alexandre Siqueira Guedes
Steindorff, Andrei Stecca
de Siqueira, Saulo José Linhares
Silva, Roberto do Nascimento
Ulhoa, Cirano José
author_facet Vieira, Pabline Marinho
Coelho, Alexandre Siqueira Guedes
Steindorff, Andrei Stecca
de Siqueira, Saulo José Linhares
Silva, Roberto do Nascimento
Ulhoa, Cirano José
author_sort Vieira, Pabline Marinho
collection PubMed
description BACKGROUND: The species of T. harzianum are well known for their biocontrol activity against many plant pathogens. However, there is a lack of studies concerning its use as a biological control agent against F. solani, a pathogen involved in several crop diseases. In this study, we have used subtractive library hybridization (SSH) and quantitative real-time PCR (RT-qPCR) techniques in order to explore changes in T. harzianum genes expression during growth on cell wall of F. solani (FSCW) or glucose. RT-qPCR was also used to examine the regulation of 18 genes, potentially involved in biocontrol, during confrontation between T. harzianum and F. solani. RESULTS: Data obtained from two subtractive libraries were compared after annotation using the Blast2GO suite. A total of 417 and 78 readable EST sequence were annotated in the FSCW and glucose libraries, respectively. Functional annotation of these genes identified diverse biological processes and molecular functions required during T. harzianum growth on FSCW or glucose. We identified various genes of biotechnological value encoding to proteins which function such as transporters, hydrolytic activity, adherence, appressorium development and pathogenesis. Fifteen genes were up-regulated and sixteen were down-regulated at least at one-time point during growth of T. harzianum in FSCW. During the confrontation assay most of the genes were up-regulated, mainly after contact, when the interaction has been established. CONCLUSIONS: This study demonstrates that T. harzianum expressed different genes when grown on FSCW compared to glucose. It provides insights into the mechanisms of gene expression involved in mycoparasitism of T. harzianum against F. solani. The identification and evaluation of these genes may contribute to the development of an efficient biological control agent.
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spelling pubmed-36066052013-03-24 Identification of differentially expressed genes from Trichoderma harzianum during growth on cell wall of Fusarium solani as a tool for biotechnological application Vieira, Pabline Marinho Coelho, Alexandre Siqueira Guedes Steindorff, Andrei Stecca de Siqueira, Saulo José Linhares Silva, Roberto do Nascimento Ulhoa, Cirano José BMC Genomics Research Article BACKGROUND: The species of T. harzianum are well known for their biocontrol activity against many plant pathogens. However, there is a lack of studies concerning its use as a biological control agent against F. solani, a pathogen involved in several crop diseases. In this study, we have used subtractive library hybridization (SSH) and quantitative real-time PCR (RT-qPCR) techniques in order to explore changes in T. harzianum genes expression during growth on cell wall of F. solani (FSCW) or glucose. RT-qPCR was also used to examine the regulation of 18 genes, potentially involved in biocontrol, during confrontation between T. harzianum and F. solani. RESULTS: Data obtained from two subtractive libraries were compared after annotation using the Blast2GO suite. A total of 417 and 78 readable EST sequence were annotated in the FSCW and glucose libraries, respectively. Functional annotation of these genes identified diverse biological processes and molecular functions required during T. harzianum growth on FSCW or glucose. We identified various genes of biotechnological value encoding to proteins which function such as transporters, hydrolytic activity, adherence, appressorium development and pathogenesis. Fifteen genes were up-regulated and sixteen were down-regulated at least at one-time point during growth of T. harzianum in FSCW. During the confrontation assay most of the genes were up-regulated, mainly after contact, when the interaction has been established. CONCLUSIONS: This study demonstrates that T. harzianum expressed different genes when grown on FSCW compared to glucose. It provides insights into the mechanisms of gene expression involved in mycoparasitism of T. harzianum against F. solani. The identification and evaluation of these genes may contribute to the development of an efficient biological control agent. BioMed Central 2013-03-15 /pmc/articles/PMC3606605/ /pubmed/23497274 http://dx.doi.org/10.1186/1471-2164-14-177 Text en Copyright ©2013 Vieira et al.; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Vieira, Pabline Marinho
Coelho, Alexandre Siqueira Guedes
Steindorff, Andrei Stecca
de Siqueira, Saulo José Linhares
Silva, Roberto do Nascimento
Ulhoa, Cirano José
Identification of differentially expressed genes from Trichoderma harzianum during growth on cell wall of Fusarium solani as a tool for biotechnological application
title Identification of differentially expressed genes from Trichoderma harzianum during growth on cell wall of Fusarium solani as a tool for biotechnological application
title_full Identification of differentially expressed genes from Trichoderma harzianum during growth on cell wall of Fusarium solani as a tool for biotechnological application
title_fullStr Identification of differentially expressed genes from Trichoderma harzianum during growth on cell wall of Fusarium solani as a tool for biotechnological application
title_full_unstemmed Identification of differentially expressed genes from Trichoderma harzianum during growth on cell wall of Fusarium solani as a tool for biotechnological application
title_short Identification of differentially expressed genes from Trichoderma harzianum during growth on cell wall of Fusarium solani as a tool for biotechnological application
title_sort identification of differentially expressed genes from trichoderma harzianum during growth on cell wall of fusarium solani as a tool for biotechnological application
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3606605/
https://www.ncbi.nlm.nih.gov/pubmed/23497274
http://dx.doi.org/10.1186/1471-2164-14-177
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