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Inhibitory Effects of Traditional Herbal Formula Pyungwi-San on Inflammatory Response In Vitro and In Vivo

Pyungwi-san (PWS) is a traditional basic herbal formula. We investigated the effects of PWS on induction of cyclooxygenase-2 (COX-2), inducible nitric oxide synthase (iNOS), pro-inflammatory cytokines (interleukin-6 (IL-6) and tumor necrosis factor-α (TNF-α)) and nuclear factor-kappa B (NF-κB) as we...

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Autores principales: Cha, Ji Young, Jung, Ji Yun, Jung, Jae Yup, Lee, Jong Rok, Cho, Il Je, Ku, Sae Kwang, Byun, Sung Hui, Ahn, Yong-Tae, Lee, Chul Won, Kim, Sang Chan, An, Won G.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi Publishing Corporation 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3606716/
https://www.ncbi.nlm.nih.gov/pubmed/23533508
http://dx.doi.org/10.1155/2013/630198
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author Cha, Ji Young
Jung, Ji Yun
Jung, Jae Yup
Lee, Jong Rok
Cho, Il Je
Ku, Sae Kwang
Byun, Sung Hui
Ahn, Yong-Tae
Lee, Chul Won
Kim, Sang Chan
An, Won G.
author_facet Cha, Ji Young
Jung, Ji Yun
Jung, Jae Yup
Lee, Jong Rok
Cho, Il Je
Ku, Sae Kwang
Byun, Sung Hui
Ahn, Yong-Tae
Lee, Chul Won
Kim, Sang Chan
An, Won G.
author_sort Cha, Ji Young
collection PubMed
description Pyungwi-san (PWS) is a traditional basic herbal formula. We investigated the effects of PWS on induction of cyclooxygenase-2 (COX-2), inducible nitric oxide synthase (iNOS), pro-inflammatory cytokines (interleukin-6 (IL-6) and tumor necrosis factor-α (TNF-α)) and nuclear factor-kappa B (NF-κB) as well as mitogen-activated protein kinases (MAPKs) in lipopolysaccharide-(LPS-) induced Raw 264.7 cells and on paw edema in rats. Treatment with PWS (0.5, 0.75, and 1 mg/mL) resulted in inhibited levels of expression of LPS-induced COX-2, iNOS, NF-κB, and MAPKs as well as production of prostaglandin E(2) (PGE(2)), nitric oxide (NO), IL-6, and TNF-α induced by LPS. Our results demonstrate that PWS possesses anti-inflammatory activities via decreasing production of pro-inflammatory mediators through suppression of the signaling pathways of NF-κB and MAPKs in LPS-induced macrophage cells. More importantly, results of the carrageenan-(CA-) induced paw edema demonstrate an anti-edema effect of PWS. In addition, it is considered that PWS also inhibits the acute edematous inflammations through suppression of mast cell degranulations and inflammatory mediators, including COX-2, iNOS and TNF-α. Thus, our findings may provide scientific evidence to explain the anti-inflammatory properties of PWS in vitro and in vivo.
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spelling pubmed-36067162013-03-26 Inhibitory Effects of Traditional Herbal Formula Pyungwi-San on Inflammatory Response In Vitro and In Vivo Cha, Ji Young Jung, Ji Yun Jung, Jae Yup Lee, Jong Rok Cho, Il Je Ku, Sae Kwang Byun, Sung Hui Ahn, Yong-Tae Lee, Chul Won Kim, Sang Chan An, Won G. Evid Based Complement Alternat Med Research Article Pyungwi-san (PWS) is a traditional basic herbal formula. We investigated the effects of PWS on induction of cyclooxygenase-2 (COX-2), inducible nitric oxide synthase (iNOS), pro-inflammatory cytokines (interleukin-6 (IL-6) and tumor necrosis factor-α (TNF-α)) and nuclear factor-kappa B (NF-κB) as well as mitogen-activated protein kinases (MAPKs) in lipopolysaccharide-(LPS-) induced Raw 264.7 cells and on paw edema in rats. Treatment with PWS (0.5, 0.75, and 1 mg/mL) resulted in inhibited levels of expression of LPS-induced COX-2, iNOS, NF-κB, and MAPKs as well as production of prostaglandin E(2) (PGE(2)), nitric oxide (NO), IL-6, and TNF-α induced by LPS. Our results demonstrate that PWS possesses anti-inflammatory activities via decreasing production of pro-inflammatory mediators through suppression of the signaling pathways of NF-κB and MAPKs in LPS-induced macrophage cells. More importantly, results of the carrageenan-(CA-) induced paw edema demonstrate an anti-edema effect of PWS. In addition, it is considered that PWS also inhibits the acute edematous inflammations through suppression of mast cell degranulations and inflammatory mediators, including COX-2, iNOS and TNF-α. Thus, our findings may provide scientific evidence to explain the anti-inflammatory properties of PWS in vitro and in vivo. Hindawi Publishing Corporation 2013 2013-03-06 /pmc/articles/PMC3606716/ /pubmed/23533508 http://dx.doi.org/10.1155/2013/630198 Text en Copyright © 2013 Ji Young Cha et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Cha, Ji Young
Jung, Ji Yun
Jung, Jae Yup
Lee, Jong Rok
Cho, Il Je
Ku, Sae Kwang
Byun, Sung Hui
Ahn, Yong-Tae
Lee, Chul Won
Kim, Sang Chan
An, Won G.
Inhibitory Effects of Traditional Herbal Formula Pyungwi-San on Inflammatory Response In Vitro and In Vivo
title Inhibitory Effects of Traditional Herbal Formula Pyungwi-San on Inflammatory Response In Vitro and In Vivo
title_full Inhibitory Effects of Traditional Herbal Formula Pyungwi-San on Inflammatory Response In Vitro and In Vivo
title_fullStr Inhibitory Effects of Traditional Herbal Formula Pyungwi-San on Inflammatory Response In Vitro and In Vivo
title_full_unstemmed Inhibitory Effects of Traditional Herbal Formula Pyungwi-San on Inflammatory Response In Vitro and In Vivo
title_short Inhibitory Effects of Traditional Herbal Formula Pyungwi-San on Inflammatory Response In Vitro and In Vivo
title_sort inhibitory effects of traditional herbal formula pyungwi-san on inflammatory response in vitro and in vivo
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3606716/
https://www.ncbi.nlm.nih.gov/pubmed/23533508
http://dx.doi.org/10.1155/2013/630198
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