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Oxidized Extracellular DNA as a Stress Signal in Human Cells

The term “cell-free DNA” (cfDNA) was recently coined for DNA fragments from plasma/serum, while DNA present in in vitro cell culture media is known as extracellular DNA (ecDNA). Under oxidative stress conditions, the levels of oxidative modification of cellular DNA and the rate of cell death increas...

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Autores principales: Ermakov, Aleksei V., Konkova, Marina S., Kostyuk, Svetlana V., Izevskaya, Vera L., Baranova, Ancha, Veiko, Natalya N.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi Publishing Corporation 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3606786/
https://www.ncbi.nlm.nih.gov/pubmed/23533696
http://dx.doi.org/10.1155/2013/649747
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author Ermakov, Aleksei V.
Konkova, Marina S.
Kostyuk, Svetlana V.
Izevskaya, Vera L.
Baranova, Ancha
Veiko, Natalya N.
author_facet Ermakov, Aleksei V.
Konkova, Marina S.
Kostyuk, Svetlana V.
Izevskaya, Vera L.
Baranova, Ancha
Veiko, Natalya N.
author_sort Ermakov, Aleksei V.
collection PubMed
description The term “cell-free DNA” (cfDNA) was recently coined for DNA fragments from plasma/serum, while DNA present in in vitro cell culture media is known as extracellular DNA (ecDNA). Under oxidative stress conditions, the levels of oxidative modification of cellular DNA and the rate of cell death increase. Dying cells release their damaged DNA, thus, contributing oxidized DNA fragments to the pool of cfDNA/ecDNA. Oxidized cell-free DNA could serve as a stress signal that promotes irradiation-induced bystander effect. Evidence points to TLR9 as a possible candidate for oxidized DNA sensor. An exposure to oxidized ecDNA stimulates a synthesis of reactive oxygen species (ROS) that evokes an adaptive response that includes transposition of the homologous loci within the nucleus, polymerization and the formation of the stress fibers of the actin, as well as activation of the ribosomal gene expression, and nuclear translocation of NF-E2 related factor-2 (NRF2) that, in turn, mediates induction of phase II detoxifying and antioxidant enzymes. In conclusion, the oxidized DNA is a stress signal released in response to oxidative stress in the cultured cells and, possibly, in the human body; in particular, it might contribute to systemic abscopal effects of localized irradiation treatments.
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spelling pubmed-36067862013-03-26 Oxidized Extracellular DNA as a Stress Signal in Human Cells Ermakov, Aleksei V. Konkova, Marina S. Kostyuk, Svetlana V. Izevskaya, Vera L. Baranova, Ancha Veiko, Natalya N. Oxid Med Cell Longev Review Article The term “cell-free DNA” (cfDNA) was recently coined for DNA fragments from plasma/serum, while DNA present in in vitro cell culture media is known as extracellular DNA (ecDNA). Under oxidative stress conditions, the levels of oxidative modification of cellular DNA and the rate of cell death increase. Dying cells release their damaged DNA, thus, contributing oxidized DNA fragments to the pool of cfDNA/ecDNA. Oxidized cell-free DNA could serve as a stress signal that promotes irradiation-induced bystander effect. Evidence points to TLR9 as a possible candidate for oxidized DNA sensor. An exposure to oxidized ecDNA stimulates a synthesis of reactive oxygen species (ROS) that evokes an adaptive response that includes transposition of the homologous loci within the nucleus, polymerization and the formation of the stress fibers of the actin, as well as activation of the ribosomal gene expression, and nuclear translocation of NF-E2 related factor-2 (NRF2) that, in turn, mediates induction of phase II detoxifying and antioxidant enzymes. In conclusion, the oxidized DNA is a stress signal released in response to oxidative stress in the cultured cells and, possibly, in the human body; in particular, it might contribute to systemic abscopal effects of localized irradiation treatments. Hindawi Publishing Corporation 2013 2013-03-06 /pmc/articles/PMC3606786/ /pubmed/23533696 http://dx.doi.org/10.1155/2013/649747 Text en Copyright © 2013 Aleksei V. Ermakov et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Review Article
Ermakov, Aleksei V.
Konkova, Marina S.
Kostyuk, Svetlana V.
Izevskaya, Vera L.
Baranova, Ancha
Veiko, Natalya N.
Oxidized Extracellular DNA as a Stress Signal in Human Cells
title Oxidized Extracellular DNA as a Stress Signal in Human Cells
title_full Oxidized Extracellular DNA as a Stress Signal in Human Cells
title_fullStr Oxidized Extracellular DNA as a Stress Signal in Human Cells
title_full_unstemmed Oxidized Extracellular DNA as a Stress Signal in Human Cells
title_short Oxidized Extracellular DNA as a Stress Signal in Human Cells
title_sort oxidized extracellular dna as a stress signal in human cells
topic Review Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3606786/
https://www.ncbi.nlm.nih.gov/pubmed/23533696
http://dx.doi.org/10.1155/2013/649747
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