Pituitary Adenoma Nitroproteomics: Current Status and Perspectives

Oxidative stress is extensively associated with tumorigenesis. A series of studies on stable tyrosine nitration as a marker of oxidative damage were performed in human pituitary and adenoma. This paper reviews published research on the mass spectrometry characteristics of nitropeptides and nitroprot...

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Autores principales: Zhan, Xianquan, Wang, Xiaowei, Desiderio, Dominic M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi Publishing Corporation 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3606787/
https://www.ncbi.nlm.nih.gov/pubmed/23533694
http://dx.doi.org/10.1155/2013/580710
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author Zhan, Xianquan
Wang, Xiaowei
Desiderio, Dominic M.
author_facet Zhan, Xianquan
Wang, Xiaowei
Desiderio, Dominic M.
author_sort Zhan, Xianquan
collection PubMed
description Oxidative stress is extensively associated with tumorigenesis. A series of studies on stable tyrosine nitration as a marker of oxidative damage were performed in human pituitary and adenoma. This paper reviews published research on the mass spectrometry characteristics of nitropeptides and nitroproteomics of pituitary controls and adenomas. The methodology used for nitroproteomics, the current status of human pituitary nitroproteomics studies, and the future perspectives are reviewed. Enrichment of those low-abundance endogenous nitroproteins from human tissues or body fluid samples is the first important step for nitroproteomics studies. Mass spectrometry is the essential approach to determine the amino acid sequence and locate the nitrotyrosine sites. Bioinformatics analyses, including protein domain and motif analyses, are needed to locate the nitrotyrosine site within the corresponding protein domains/motifs. Systems biology techniques, including pathway analysis, are necessary to discover signaling pathway networks involving nitroproteins from the systematically global point of view. Future quantitative nitroproteomics will discover pituitary adenoma-specific nitroprotein(s). Structural biology techniques such as X-ray crystallography analysis will solidly clarify the effects of tyrosine nitration on structure and functions of a protein. Those studies will eventually address the mechanisms and biological functions of tyrosine nitration in pituitary tumorigenesis and will discover nitroprotein biomarkers for pituitary adenomas and targets for drug design for pituitary adenoma therapy.
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spelling pubmed-36067872013-03-26 Pituitary Adenoma Nitroproteomics: Current Status and Perspectives Zhan, Xianquan Wang, Xiaowei Desiderio, Dominic M. Oxid Med Cell Longev Review Article Oxidative stress is extensively associated with tumorigenesis. A series of studies on stable tyrosine nitration as a marker of oxidative damage were performed in human pituitary and adenoma. This paper reviews published research on the mass spectrometry characteristics of nitropeptides and nitroproteomics of pituitary controls and adenomas. The methodology used for nitroproteomics, the current status of human pituitary nitroproteomics studies, and the future perspectives are reviewed. Enrichment of those low-abundance endogenous nitroproteins from human tissues or body fluid samples is the first important step for nitroproteomics studies. Mass spectrometry is the essential approach to determine the amino acid sequence and locate the nitrotyrosine sites. Bioinformatics analyses, including protein domain and motif analyses, are needed to locate the nitrotyrosine site within the corresponding protein domains/motifs. Systems biology techniques, including pathway analysis, are necessary to discover signaling pathway networks involving nitroproteins from the systematically global point of view. Future quantitative nitroproteomics will discover pituitary adenoma-specific nitroprotein(s). Structural biology techniques such as X-ray crystallography analysis will solidly clarify the effects of tyrosine nitration on structure and functions of a protein. Those studies will eventually address the mechanisms and biological functions of tyrosine nitration in pituitary tumorigenesis and will discover nitroprotein biomarkers for pituitary adenomas and targets for drug design for pituitary adenoma therapy. Hindawi Publishing Corporation 2013 2013-03-07 /pmc/articles/PMC3606787/ /pubmed/23533694 http://dx.doi.org/10.1155/2013/580710 Text en Copyright © 2013 Xianquan Zhan et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Review Article
Zhan, Xianquan
Wang, Xiaowei
Desiderio, Dominic M.
Pituitary Adenoma Nitroproteomics: Current Status and Perspectives
title Pituitary Adenoma Nitroproteomics: Current Status and Perspectives
title_full Pituitary Adenoma Nitroproteomics: Current Status and Perspectives
title_fullStr Pituitary Adenoma Nitroproteomics: Current Status and Perspectives
title_full_unstemmed Pituitary Adenoma Nitroproteomics: Current Status and Perspectives
title_short Pituitary Adenoma Nitroproteomics: Current Status and Perspectives
title_sort pituitary adenoma nitroproteomics: current status and perspectives
topic Review Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3606787/
https://www.ncbi.nlm.nih.gov/pubmed/23533694
http://dx.doi.org/10.1155/2013/580710
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