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Sustained and Transient Contributions to the Rat Dark-Adapted Electroretinogram b-Wave

The most dominant feature of the electroretinogram, the b-wave, is thought to reflect ON-bipolar cell responses. However, a number of studies suggest that the b-wave is made up of several components. We consider the composition of the rat b-wave by subtracting corneal negative components obtained us...

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Autores principales: Dang, Trung M., Vingrys, Algis J., Bui, Bang V.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi Publishing Corporation 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3606803/
https://www.ncbi.nlm.nih.gov/pubmed/23533706
http://dx.doi.org/10.1155/2013/352917
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author Dang, Trung M.
Vingrys, Algis J.
Bui, Bang V.
author_facet Dang, Trung M.
Vingrys, Algis J.
Bui, Bang V.
author_sort Dang, Trung M.
collection PubMed
description The most dominant feature of the electroretinogram, the b-wave, is thought to reflect ON-bipolar cell responses. However, a number of studies suggest that the b-wave is made up of several components. We consider the composition of the rat b-wave by subtracting corneal negative components obtained using intravitreal application of pharmacological agents to remove postreceptoral responses. By analyzing the intensity-response characteristic of the PII across a range of fixed times during and after a light step, we find that the rat isolated PII has 2 components. The first has fast rise and decay characteristics with a low sensitivity to light. GABAc-mediated inhibitory pathways enhance this transient-ON component to manifest increased and deceased sensitivity to light at shorter (<160 ms) and longer times, respectively. The second component has slower temporal characteristics but is more sensitive to light. GABAc-mediated inhibition enhances this sustained-ON component but has little effect on its sensitivity to light. After stimulus offset, both transient and sustained components return to baseline, and a long latency sustained positive component becomes apparent. The light sensitivities of transient-ON and sustained-OFF components are consistent with activity arising from cone ON- and OFF-bipolar cells, whereas the sustained-ON component is likely to arise from rod bipolar cells.
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spelling pubmed-36068032013-03-26 Sustained and Transient Contributions to the Rat Dark-Adapted Electroretinogram b-Wave Dang, Trung M. Vingrys, Algis J. Bui, Bang V. J Ophthalmol Research Article The most dominant feature of the electroretinogram, the b-wave, is thought to reflect ON-bipolar cell responses. However, a number of studies suggest that the b-wave is made up of several components. We consider the composition of the rat b-wave by subtracting corneal negative components obtained using intravitreal application of pharmacological agents to remove postreceptoral responses. By analyzing the intensity-response characteristic of the PII across a range of fixed times during and after a light step, we find that the rat isolated PII has 2 components. The first has fast rise and decay characteristics with a low sensitivity to light. GABAc-mediated inhibitory pathways enhance this transient-ON component to manifest increased and deceased sensitivity to light at shorter (<160 ms) and longer times, respectively. The second component has slower temporal characteristics but is more sensitive to light. GABAc-mediated inhibition enhances this sustained-ON component but has little effect on its sensitivity to light. After stimulus offset, both transient and sustained components return to baseline, and a long latency sustained positive component becomes apparent. The light sensitivities of transient-ON and sustained-OFF components are consistent with activity arising from cone ON- and OFF-bipolar cells, whereas the sustained-ON component is likely to arise from rod bipolar cells. Hindawi Publishing Corporation 2013 2013-03-06 /pmc/articles/PMC3606803/ /pubmed/23533706 http://dx.doi.org/10.1155/2013/352917 Text en Copyright © 2013 Trung M. Dang et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Dang, Trung M.
Vingrys, Algis J.
Bui, Bang V.
Sustained and Transient Contributions to the Rat Dark-Adapted Electroretinogram b-Wave
title Sustained and Transient Contributions to the Rat Dark-Adapted Electroretinogram b-Wave
title_full Sustained and Transient Contributions to the Rat Dark-Adapted Electroretinogram b-Wave
title_fullStr Sustained and Transient Contributions to the Rat Dark-Adapted Electroretinogram b-Wave
title_full_unstemmed Sustained and Transient Contributions to the Rat Dark-Adapted Electroretinogram b-Wave
title_short Sustained and Transient Contributions to the Rat Dark-Adapted Electroretinogram b-Wave
title_sort sustained and transient contributions to the rat dark-adapted electroretinogram b-wave
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3606803/
https://www.ncbi.nlm.nih.gov/pubmed/23533706
http://dx.doi.org/10.1155/2013/352917
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