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Sustained and Transient Contributions to the Rat Dark-Adapted Electroretinogram b-Wave
The most dominant feature of the electroretinogram, the b-wave, is thought to reflect ON-bipolar cell responses. However, a number of studies suggest that the b-wave is made up of several components. We consider the composition of the rat b-wave by subtracting corneal negative components obtained us...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Hindawi Publishing Corporation
2013
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3606803/ https://www.ncbi.nlm.nih.gov/pubmed/23533706 http://dx.doi.org/10.1155/2013/352917 |
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author | Dang, Trung M. Vingrys, Algis J. Bui, Bang V. |
author_facet | Dang, Trung M. Vingrys, Algis J. Bui, Bang V. |
author_sort | Dang, Trung M. |
collection | PubMed |
description | The most dominant feature of the electroretinogram, the b-wave, is thought to reflect ON-bipolar cell responses. However, a number of studies suggest that the b-wave is made up of several components. We consider the composition of the rat b-wave by subtracting corneal negative components obtained using intravitreal application of pharmacological agents to remove postreceptoral responses. By analyzing the intensity-response characteristic of the PII across a range of fixed times during and after a light step, we find that the rat isolated PII has 2 components. The first has fast rise and decay characteristics with a low sensitivity to light. GABAc-mediated inhibitory pathways enhance this transient-ON component to manifest increased and deceased sensitivity to light at shorter (<160 ms) and longer times, respectively. The second component has slower temporal characteristics but is more sensitive to light. GABAc-mediated inhibition enhances this sustained-ON component but has little effect on its sensitivity to light. After stimulus offset, both transient and sustained components return to baseline, and a long latency sustained positive component becomes apparent. The light sensitivities of transient-ON and sustained-OFF components are consistent with activity arising from cone ON- and OFF-bipolar cells, whereas the sustained-ON component is likely to arise from rod bipolar cells. |
format | Online Article Text |
id | pubmed-3606803 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2013 |
publisher | Hindawi Publishing Corporation |
record_format | MEDLINE/PubMed |
spelling | pubmed-36068032013-03-26 Sustained and Transient Contributions to the Rat Dark-Adapted Electroretinogram b-Wave Dang, Trung M. Vingrys, Algis J. Bui, Bang V. J Ophthalmol Research Article The most dominant feature of the electroretinogram, the b-wave, is thought to reflect ON-bipolar cell responses. However, a number of studies suggest that the b-wave is made up of several components. We consider the composition of the rat b-wave by subtracting corneal negative components obtained using intravitreal application of pharmacological agents to remove postreceptoral responses. By analyzing the intensity-response characteristic of the PII across a range of fixed times during and after a light step, we find that the rat isolated PII has 2 components. The first has fast rise and decay characteristics with a low sensitivity to light. GABAc-mediated inhibitory pathways enhance this transient-ON component to manifest increased and deceased sensitivity to light at shorter (<160 ms) and longer times, respectively. The second component has slower temporal characteristics but is more sensitive to light. GABAc-mediated inhibition enhances this sustained-ON component but has little effect on its sensitivity to light. After stimulus offset, both transient and sustained components return to baseline, and a long latency sustained positive component becomes apparent. The light sensitivities of transient-ON and sustained-OFF components are consistent with activity arising from cone ON- and OFF-bipolar cells, whereas the sustained-ON component is likely to arise from rod bipolar cells. Hindawi Publishing Corporation 2013 2013-03-06 /pmc/articles/PMC3606803/ /pubmed/23533706 http://dx.doi.org/10.1155/2013/352917 Text en Copyright © 2013 Trung M. Dang et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Dang, Trung M. Vingrys, Algis J. Bui, Bang V. Sustained and Transient Contributions to the Rat Dark-Adapted Electroretinogram b-Wave |
title | Sustained and Transient Contributions to the Rat Dark-Adapted Electroretinogram b-Wave |
title_full | Sustained and Transient Contributions to the Rat Dark-Adapted Electroretinogram b-Wave |
title_fullStr | Sustained and Transient Contributions to the Rat Dark-Adapted Electroretinogram b-Wave |
title_full_unstemmed | Sustained and Transient Contributions to the Rat Dark-Adapted Electroretinogram b-Wave |
title_short | Sustained and Transient Contributions to the Rat Dark-Adapted Electroretinogram b-Wave |
title_sort | sustained and transient contributions to the rat dark-adapted electroretinogram b-wave |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3606803/ https://www.ncbi.nlm.nih.gov/pubmed/23533706 http://dx.doi.org/10.1155/2013/352917 |
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