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Novel Nanosomes for Gene Delivery to Plasmodium falciparum-infected Red Blood Cells

Malaria threatens millions of people annually and is a burden to human health and economic development. Unfortunately in terms of disease control, no effective vaccines are available and the efficacy of treatment is limited by drug resistance. Genetic manipulation in Plasmodium falciparum is hampere...

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Autores principales: Gopalakrishnan, Anusha M., Kundu, Anup K., Mandal, Tarun K., Kumar, Nirbhay
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3607119/
https://www.ncbi.nlm.nih.gov/pubmed/23525038
http://dx.doi.org/10.1038/srep01534
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author Gopalakrishnan, Anusha M.
Kundu, Anup K.
Mandal, Tarun K.
Kumar, Nirbhay
author_facet Gopalakrishnan, Anusha M.
Kundu, Anup K.
Mandal, Tarun K.
Kumar, Nirbhay
author_sort Gopalakrishnan, Anusha M.
collection PubMed
description Malaria threatens millions of people annually and is a burden to human health and economic development. Unfortunately in terms of disease control, no effective vaccines are available and the efficacy of treatment is limited by drug resistance. Genetic manipulation in Plasmodium falciparum is hampered due to the absence of robust methods for genetic analyses. Electroporation-based transfection methods have allowed the study of gene function in P. falciparum, with low efficiency. A lipid nanoparticle was developed that allowed nuclear targeting of pDNA with increased efficiency in reporter assay, compared to traditional electroporation method. This method has for the first time, facilitated transfection using both circular and linear DNA in P. falciparum thereby serving as an alternative to electroporation with an increase in transfection efficiency. Availability of a robust method for functional genomic studies in these organisms may be a catalyst for discovery of novel targets for developing drugs and vaccines.
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spelling pubmed-36071192013-03-25 Novel Nanosomes for Gene Delivery to Plasmodium falciparum-infected Red Blood Cells Gopalakrishnan, Anusha M. Kundu, Anup K. Mandal, Tarun K. Kumar, Nirbhay Sci Rep Article Malaria threatens millions of people annually and is a burden to human health and economic development. Unfortunately in terms of disease control, no effective vaccines are available and the efficacy of treatment is limited by drug resistance. Genetic manipulation in Plasmodium falciparum is hampered due to the absence of robust methods for genetic analyses. Electroporation-based transfection methods have allowed the study of gene function in P. falciparum, with low efficiency. A lipid nanoparticle was developed that allowed nuclear targeting of pDNA with increased efficiency in reporter assay, compared to traditional electroporation method. This method has for the first time, facilitated transfection using both circular and linear DNA in P. falciparum thereby serving as an alternative to electroporation with an increase in transfection efficiency. Availability of a robust method for functional genomic studies in these organisms may be a catalyst for discovery of novel targets for developing drugs and vaccines. Nature Publishing Group 2013-03-25 /pmc/articles/PMC3607119/ /pubmed/23525038 http://dx.doi.org/10.1038/srep01534 Text en Copyright © 2013, Macmillan Publishers Limited. All rights reserved http://creativecommons.org/licenses/by-nc-nd/3.0/ This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivs 3.0 Unported License. To view a copy of this license, visit http://creativecommons.org/licenses/by-nc-nd/3.0/
spellingShingle Article
Gopalakrishnan, Anusha M.
Kundu, Anup K.
Mandal, Tarun K.
Kumar, Nirbhay
Novel Nanosomes for Gene Delivery to Plasmodium falciparum-infected Red Blood Cells
title Novel Nanosomes for Gene Delivery to Plasmodium falciparum-infected Red Blood Cells
title_full Novel Nanosomes for Gene Delivery to Plasmodium falciparum-infected Red Blood Cells
title_fullStr Novel Nanosomes for Gene Delivery to Plasmodium falciparum-infected Red Blood Cells
title_full_unstemmed Novel Nanosomes for Gene Delivery to Plasmodium falciparum-infected Red Blood Cells
title_short Novel Nanosomes for Gene Delivery to Plasmodium falciparum-infected Red Blood Cells
title_sort novel nanosomes for gene delivery to plasmodium falciparum-infected red blood cells
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3607119/
https://www.ncbi.nlm.nih.gov/pubmed/23525038
http://dx.doi.org/10.1038/srep01534
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