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Ubiquinone-quantum dot bioconjugates for in vitro and intracellular complex I sensing

Quantum dots (QDs) have attracted increasing interest in bioimaging and sensing. Here, we report a biosensor of complex I using ubiquinone-terminated disulphides with different alkyl spacers (Q(n)NS, n = 2, 5 and 10) as surface-capping ligands to functionalise CdSe/ZnS QDs. The enhancement or quench...

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Detalles Bibliográficos
Autores principales: Ma, Wei, Qin, Li-Xia, Liu, Feng-Tao, Gu, Zhen, Wang, Jian, Pan, Zhi Gang, James, Tony D., Long, Yi-Tao
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3607194/
https://www.ncbi.nlm.nih.gov/pubmed/23524384
http://dx.doi.org/10.1038/srep01537
Descripción
Sumario:Quantum dots (QDs) have attracted increasing interest in bioimaging and sensing. Here, we report a biosensor of complex I using ubiquinone-terminated disulphides with different alkyl spacers (Q(n)NS, n = 2, 5 and 10) as surface-capping ligands to functionalise CdSe/ZnS QDs. The enhancement or quenching of the QD bioconjugates fluorescence changes as a function of the redox state of Q(n)NS, since QDs are highly sensitive to the electron-transfer processes. The bioconjugated Q(n)NS-QDs emission could be modulated by complex I in the presence of NADH, which simulates an electron-transfer system part of the mitochondrial respiratory chain, providing an in vitro and intracellular complex I sensor. Epidemiological studies suggest that Parkinson's patients have the impaired activity of complex I in the electron-transfer chain of mitochondria. We have demonstrated that the Q(n)NS-QDs system could aid in early stage Parkinson's disease diagnosis and progression monitoring by following different complex I levels in SH-SY5Y cells.